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          Clicking the images or links will redirect you to a website hosted by BenchSci that provides third-party scientific content. Neither the content nor the BenchSci technology and processes for selection have been evaluated by us; we are providing them as-is and without warranty of any kind, including for use or application of the Thermo Fisher Scientific products presented.

          • Primary Antibodies ›
          • SERCA2 ATPase Antibodies

          Invitrogen

          SERCA2 ATPase Monoclonal Antibody (IID8)

          Advanced Verification
          29 Published Figures
          146 References
          View all (21) SERCA2 ATPase antibodies
          Datasheet
          Protocols
          Questions & Answers
          Datasheet
          Protocols
          Questions & Answers

          Cite SERCA2 ATPase Monoclonal Antibody (IID8)

          • Antibody Testing Data (9)
          • Published Figures (29)
          • Advanced Verification (1)
          SERCA2 ATPase Antibody in Immunocytochemistry (ICC/IF)
          Group 53 Created with Sketch.
          SERCA2 ATPase Antibody in Immunocytochemistry (ICC/IF)
          Group 53 Created with Sketch.

          FIGURE: 1 / 39

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          SERCA2 ATPase Antibody (MA3-910) in ICC/IF

          Immunofluorescence analysis of SERCA2 was performed using 70% confluent log phase MDCK cells. The cells were fixed with 4% paraformaldehyde for 10 minutes, permeabilized with 0.1% Triton™ X-100 for 15 minutes, and blocked with 2% BSA for 1 hour at room temperature. The cells were labeled with SERCA2 ATPase Monoclonal Antibody (Product # MA3-910) at 5 µg/mL in 0.1% BSA, incubated at 4 degree celsius overnigh... View More {{ $ctrl.currentElement.advancedVerification.fullName }} validation info. View more
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          View Product

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          SERCA2 ATPase Antibody in Immunocytochemistry (ICC/IF)
          SERCA2 ATPase Antibody in Immunocytochemistry (ICC/IF)
          SERCA2 ATPase Antibody in Immunocytochemistry (ICC/IF)
          SERCA2 ATPase Antibody in Immunocytochemistry (ICC/IF)
          SERCA2 ATPase Antibody in Immunohistochemistry (Paraffin) (IHC (P))
          SERCA2 ATPase Antibody in Immunohistochemistry (Paraffin) (IHC (P))
          SERCA2 ATPase Antibody in Immunohistochemistry (Paraffin) (IHC (P))
          SERCA2 ATPase Antibody in Western Blot (WB)
          SERCA2 ATPase Antibody in Western Blot (WB)
          SERCA2 ATPase Antibody in Immunocytochemistry (ICC/IF)
          SERCA2 ATPase Antibody in Immunocytochemistry (ICC/IF)
          SERCA2 ATPase Antibody in Western Blot, Immunocytochemistry, Immunoprecipitation (WB, ICC/IF, IP)
          SERCA2 ATPase Antibody in Immunocytochemistry (ICC/IF)
          SERCA2 ATPase Antibody in Immunocytochemistry (ICC/IF)
          SERCA2 ATPase Antibody in Immunocytochemistry (ICC/IF)
          SERCA2 ATPase Antibody in Immunohistochemistry (IHC)
          SERCA2 ATPase Antibody in Western Blot, Immunoprecipitation (WB, IP)
          SERCA2 ATPase Antibody in Western Blot (WB)
          SERCA2 ATPase Antibody in Western Blot (WB)
          SERCA2 ATPase Antibody in Western Blot (WB)
          SERCA2 ATPase Antibody in Western Blot (WB)
          SERCA2 ATPase Antibody in Western Blot (WB)
          SERCA2 ATPase Antibody in Western Blot (WB)
          SERCA2 ATPase Antibody in Western Blot (WB)
          SERCA2 ATPase Antibody in Western Blot (WB)
          SERCA2 ATPase Antibody in Western Blot (WB)
          SERCA2 ATPase Antibody in Western Blot, Immunoprecipitation (WB, IP)
          SERCA2 ATPase Antibody in Western Blot (WB)
          SERCA2 ATPase Antibody in Western Blot (WB)
          SERCA2 ATPase Antibody in Western Blot (WB)
          SERCA2 ATPase Antibody in Western Blot (WB)
          SERCA2 ATPase Antibody in Western Blot (WB)
          SERCA2 ATPase Antibody in Western Blot (WB)
          SERCA2 ATPase Antibody in Western Blot (WB)
          SERCA2 ATPase Antibody in Western Blot (WB)
          SERCA2 ATPase Antibody in Western Blot (WB)
          SERCA2 ATPase Antibody in Western Blot (WB)
          SERCA2 ATPase Antibody in Mass Spectrometry (MS)
          SERCA2 ATPase Antibody

          Product Details

          MA3-910

          Applications
          Tested Dilution
          Publications

          Western Blot (WB)

          1:500-1:5,000
          View 85 publications 85 publications

          Immunohistochemistry (IHC)

          -
          View 19 publications 19 publications

          Immunohistochemistry (Paraffin) (IHC (P))

          1 µg/mL
          -

          Immunohistochemistry (Frozen) (IHC (F))

          1:500
          View 1 publication 1 publication

          Immunocytochemistry (ICC/IF)

          5 µg/mL
          View 31 publications 31 publications

          Flow Cytometry (Flow)

          1 µg / 10^6 cells
          -

          ELISA (ELISA)

          -
          View 1 publication 1 publication

          Immunoprecipitation (IP)

          -
          View 4 publications 4 publications

          ChIP assay (ChIP)

          -
          View 1 publication 1 publication

          in situ PLA (PLA)

          -
          View 1 publication 1 publication

          Immunomicroscopy (IM)

          -
          View 1 publication 1 publication

          Miscellaneous PubMed (MISC)

          -
          View 1 publication 1 publication

          Mass Spectrometry (MS)

          -
          View 1 publication 1 publication
          Product Specifications

          Species Reactivity

          Bovine, Dog, Human, Mouse, Pig, Rabbit, Rat

          Published species

          Amphibian, Dog, Guinea pig, Hamster, Human, Mouse, Non-human primate, Pig, Rabbit, Rat, Sheep, Virus, Zebrafish

          Host/Isotype

          Mouse / IgG1

          Class

          Monoclonal

          Type

          Antibody

          Clone

          IID8

          Immunogen

          Purified canine cardiac sarcoplasmic reticulum.
          View immunogen

          Conjugate

          Unconjugated Unconjugated Unconjugated

          Form

          Liquid

          Concentration

          1 mg/mL

          Purification

          Affinity chromatography

          Storage buffer

          PBS

          Contains

          0.05% sodium azide

          Storage conditions

          -20°C, Avoid Freeze/Thaw Cycles

          Shipping conditions

          Ambient (domestic); Wet ice (international)

          RRID

          AB_2227681

          Product Specific Information

          MA3-910 detects sarcoplasmic or endoplasmic reticulum calcium 2 (SERCA2) ATPase from bovine, canine, human, pig, rabbit and rat tissues. This antibody does not detect frog SERCA2 ATPase (J. Biol. Chem., Feb 2001; 276: 3911 - 3919). MA3-910 detects SERCA2 ATPase and does not discriminate between the two isoforms.

          MA3-910 has been successfully used in Western blot, immunofluorescence, immunocytochemistry, and immunohistochemistry. By Western blot, this antibody detects an ~110 kDa protein representing SERCA2 ATPase from canine skeletal muscle triad preparations. Immunofluorescence staining of SECRA2 ATPase in rabbit skeletal muscle with MA3-910 results in strong labeling of the entire type I (slow) myofiber consistent with sarcoplasmic reticulum localization.

          MA3-910 is not recommended for Western blot detection of rat SERCA2.

          The MA3-910 antigen is purified canine cardiac sarcoplasmic reticulum.

          Target Information

          This gene encodes one of the SERCA Ca(2+)-ATPases, which are intracellular pumps located in the sarcoplasmic or endoplasmic reticula of muscle cells. This enzyme catalyzes the hydrolysis of ATP coupled with the translocation of calcium from the cytosol into the sarcoplasmic reticulum lumen, and is involved in regulation of the contraction/relaxation cycle. Mutations in this gene cause Darier-White disease, also known as keratosis follicularis, an autosomal dominant skin disorder characterized by loss of adhesion between epidermal cells and abnormal keratinization. Alternative splicing results in multiple transcript variants encoding different isoforms.

          For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

          Bioinformatics

          Protein Aliases: Atp2a2; ATPase Ca++ transporting cardiac muscle slow twitch 2; ATPase, Ca++ dependent, slow-twitch, cardiac muscle-2; ATPase, Ca++ transporting, cardiac muscle, slow twitch 2; ATPase, Ca++ transporting, slow twitch 2; Ca(2+)-transport ATPase class 3; calcium ATPase; Calcium pump 2; Calcium-transporting ATPase sarcoplasmic reticulum type, slow twitch skeletal muscle isoform; cardiac Ca2+ ATPase; Endoplasmic reticulum class 1/2 Ca(2+) ATPase; sarco(endo)plasmic reticulum Ca(2+)-dependent ATPase 2; sarco/endoplasmic reticulum Ca2+-ATPase 2; sarcoplasmic reticulum Ca2+-transport ATPase isoform; Sarcoplasmic/endoplasmic reticulum calcium ATPase 2; sarcoplasmic/endoplasmic-reticulum Ca(2+) pump gene 2; SERCA; SERCA ATPase; SERCA2; SR Ca(2+)-ATPase 2

          View more View less

          Gene Aliases: 9530097L16Rik; ATP2; ATP2A2; ATP2B; BOS_16598; D5Wsu150e; DAR; DD; mKIAA4195; SERCA2; SERCA2A; SERCA2B; SercaII

          View more View less

          UniProt ID: (Dog) O46674, (Rabbit) P20647, (Human) P16615, (Pig) P11607, (Mouse) O55143, (Rat) P11507

          View more View less

          Entrez Gene ID: (Dog) 403878, (Bovine) 540568, (Rabbit) 100038308, (Human) 488, (Pig) 396875, (Mouse) 11938, (Rat) 29693

          View more View less

          Function(s)
          calcium ion binding ATP binding protein C-terminus binding enzyme binding S100 protein binding calcium-transporting ATPase activity involved in regulation of cardiac muscle cell membrane potential calcium-transporting ATPase activity protein binding lutropin-choriogonadotropic hormone receptor binding nucleotide binding hydrolase activity metal ion binding
          Process(es)
          regulation of the force of heart contraction cellular calcium ion homeostasis ER-nucleus signaling pathway organelle organization metabolic process regulation of cardiac muscle contraction by calcium ion signaling transition between fast and slow fiber positive regulation of endoplasmic reticulum calcium ion concentration T-tubule organization cellular response to oxidative stress negative regulation of heart contraction relaxation of cardiac muscle sarcoplasmic reticulum calcium ion transport calcium ion transmembrane transport regulation of cardiac muscle cell membrane potential regulation of calcium ion-dependent exocytosis of neurotransmitter calcium ion transport from cytosol to endoplasmic reticulum cell adhesion epidermis development positive regulation of heart rate endoplasmic reticulum calcium ion homeostasis ion transmembrane transport response to endoplasmic reticulum stress response to peptide hormone regulation of cardiac muscle cell action potential involved in regulation of contraction regulation of cardiac conduction calcium ion import into sarcoplasmic reticulum transport ion transport calcium ion transport regulation of muscle contraction
          It has to be done as per old AB suggested Products section.
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