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Invitrogen
SERPINB2 Polyclonal Antibody
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FIGURE: 1 / 6
SERPINB2 Antibody (PA5-27857) in ICC/IF
SERPINB2 Polyclonal Antibody detects PAI2 protein at Golgi apparatus by immunofluorescent analysis. Sample: HeLa cells were fixed in 4% paraformaldehyde at RT for 15 min. Green: PAI2 protein stained by SERPINB2 Polyclonal Antibody (Product # PA5-27857) diluted at 1:500. Blue: Hoechst 33342 staining. Scale bar = 10 µm.
Product Details
PA5-27857
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Human,
Mouse
Host/Isotype
Rabbit
/ IgG
Class
Polyclonal
Type
Antibody
Immunogen
Recombinant protein encompassing a sequence within the center region of human PAI2. The exact sequence is proprietary.
Conjugate
Form
Liquid
Concentration
0.39 mg/mL
Purification
Antigen affinity chromatography
Storage buffer
PBS, pH 7, with 20% glycerol
Contains
0.025% ProClin 300
Storage conditions
Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.
Shipping conditions
Ambient (domestic); Wet ice (international)
RRID
AB_2545333
Product Specific Information
Recommended positive controls: A431, JC, U87-MG.
Predicted reactivity: Mouse (80%), Rabbit (86%), Sheep (81%), Bovine (82%).
Store product as a concentrated solution. Centrifuge briefly prior to opening the vial.
Target Information
PAI-2 is an inhibitory serpin expressed mainly in keratinocytes, activated monocytes, and placental trophoblasts. It exists predominantly as a 47 kDa, nonglycosylated, intracellular protein, which can be induced to be secreted as 60 kDa glycoprotein. The glycosylated and unglycosylated forms of PAI-2 are equally effective as inhibitors of urokinase-type plasminogen activator (uPA), the only established physiological target of this serpin. PAI-2 has a unique ability to form dormant polymers spontaneously and reversibly under physiological conditions. The physiological relevance of this property, which is neither a consequence of any mutation in the PAI-2 gene nor associated with any known disorder, is still unclear. However, it appears that the formation of intracellular, dormant polymers may be important for the controlled release of the inhibitor from PAI-2 producing cells. Plasma levels of PAI-2 are usually low or undetectable, except during pregnancy and in some forms of monocytic leukemia. Secretion of PAI-2 from the placenta normally occurs during the third trimester of pregnancy, and accounts for the dramatic increase in PAI-2 levels (up to 250 ng/ml), which are maintained at these levels until postpartum, and then rapidly decline. In addition to its vital role in protecting the placenta from degradation by uPA and/or uPA-activated proteases, PAI-2 has been shown to be essential for the prevention of metastatic spread of neck, lung and breast cancers. The beneficial effect of PAI-2 seen in these studies is presumed to stem from its ability to inhibit uPA-dependent cell dissemination. PAI-2 has also been reported to inhibit keratinocyte proliferation, and to participate in the innate immune response during viral infection.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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Bioinformatics
Protein Aliases: Monocyte Arg-serpin; PAI-2; Placental plasminogen activator inhibitor; Plasminogen activator inhibitor 2; Plasminogen activator inhibitor 2, macrophage; plasminogen activator inhibitor, type II; plasminogen activator inhibitor, type II (arginine-serpin); serine (or cysteine) proteinase inhibitor, clade B (ovalbumin), member 2; serine (or cysteine) proteinase inhibitor, clade B, member 2; Serpin B2; serpin peptidase inhibitor, clade B (ovalbumin), member 2; Urokinase inhibitor
Gene Aliases: HsT1201; ovalbumin; PAI; PAI-2; PAI2; PLANH2; SERPINB2
UniProt ID: (Human) P05120, (Mouse) P12388
Entrez Gene ID: (Human) 5055, (Mouse) 18788
Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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