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Invitrogen
SERPINH1 Polyclonal Antibody
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FIGURE: 1 / 11
SERPINH1 Antibody (PA5-95231) in ICC/IF
Immunofluorescence analysis of Serpin H1 was performed using 70% confluent log phase Caco-2 cells. The cells were fixed with 4% paraformaldehyde for 5 minutes, permeabilized with 0.1% Triton™ X-100 for 10 minutes, and blocked with 2% BSA for 45 minutes at room temperature. The cells were labeled with SERPINH1 Polyclonal Antibody (Product # PA5-95231) at 5 µg/mL concentration in 0.1% BSA, incubated at 4 degree celsius overnight and then labeled with Donkey anti-Rabbit IgG (H+L) Highly Cross-Adsorbed Secondary Antibody, Alexa Fluor Plus 488 ... View More
Product Details
PA5-95231
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Human,
Mouse,
Rat
Host/Isotype
Rabbit
/ IgG
Class
Polyclonal
Type
Antibody
Immunogen
E.coli-derived human Hsp47 recombinant protein (Position: D247-L418).
Conjugate
Form
Lyophilized
Concentration
500 µg/mL
Purification
Affinity chromatography
Storage buffer
PBS with 5mg BSA
Contains
0.05mg sodium azide
Storage conditions
-20°C
Shipping conditions
Ambient (domestic); Wet ice (international)
RRID
AB_2807035
Product Specific Information
Human Hsp47 shares 97% amino acid (aa) sequence identity with both mouse and rat Hsp47.
Reconstitute with 0.2 mL of distilled water to yield a concentration of 500 µg/mL.
Positive Control - WB: human A431 whole cell, human SK-N-SH whole cell, human Hela whole cell, rat lung tissue, rat kidney tissue, rat L6 whole cell, mouse lung tissue, mouse NIH/3T3 whole cell. IHC: human breast cancer tissue, human endometrial adenocarcinoma tissue, human esophageal squamous carcinoma tissue, human liver cancer tissue, human spleen tissue, human lung adenocarcinoma tissue.|Store at -20°C for one year from date of receipt. After reconstitution, at 4°C for one month. It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freeze-thaw cycles.
Target Information
SERPINH1 is a member of the serpin superfamily of serine proteinase inhibitors, and its expression is induced by heat shock. The SERPINH1 protein localizes to the endoplasmic reticulum lumen and binds collagen; thus it is thought to be a molecular chaperone involved in the maturation of collagen molecules. Autoantibodies to SERPINH1 have been found in patients with rheumatoid arthritis.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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Bioinformatics
Protein Aliases: 47 kDa heat shock protein; Arsenic-transactivated protein 3; AsTP 3; AsTP3; beta enolase repressor factor 1; CBP 1; CBP 2; Cell proliferation-inducing gene 14 protein; collagen binding protein 1; collagen binding protein 2; Collagen-binding protein; Colligen; Colligin; Colligin 1; Colligin 2; colligin-1; colligin-2; GP46; HGNC 1547; Hsp 47; PIG 14; RA A47; rheumatoid arthritis antigen A-47; Rheumatoid arthritis-related antigen RA-A47; serine (or cysteine) peptidase inhibitor, clade H, member 1; serine (or cysteine) proteinase inhibitor, clade H (heat shock protein 47), member 1; serine (or cysteine) proteinase inhibitor, clade H (heat shock protein 47), member 1, (collagen binding protein 1); serine (or cysteine) proteinase inhibitor, clade H (heat shock protein 47), member 2; serine (or cysteine) proteinase inhibitor, clade H (heat shock protein 47), member 2, (collagen-binding protein 2); serine (or cysteine) proteinase inhibitor, clade H, member 1; Serine protease inhibitor J6; serine proteinase inhibitor, clade H (heat shock protein 47), member 1; Serpin H1; serpin peptidase inhibitor, clade H (heat shock protein 47), member 1, (collagen binding protein 1); serpin peptidase inhibitor, clade H, member 1
Gene Aliases: AsTP3; BERF-1; CBP1; CBP2; gp46; HSP47; J6; OI10; PIG14; PPROM; RA-A47; SERPINH1; SERPINH2
UniProt ID: (Human) P50454, (Rat) P29457, (Mouse) P19324
Entrez Gene ID: (Human) 871, (Rat) 29345, (Mouse) 12406
Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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