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Invitrogen
SERPINH1 Monoclonal Antibody (1C4-1A6)
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FIGURE: 1 / 6
SERPINH1 Antibody (MA5-27711) in ICC/IF
Immunofluorescent analysis of SERPINH1 in human heat shocked HeLa cells. Sample was fixed using 2% formaldehyde (20 min at RT), incubated with SERPINH1 monoclonal antibody (Product # MA5-27711) using a dilution of 1:100 (12 hours at 4°C), and followed by APC Goat Anti-Mouse (red) at 1:200 for 2 hours at RT. Counterstain: DAPI (blue) nuclear stain at 1:40000 for 2 hours at RT. Localization: Endoplasmic reticulum lumen. Cytoplasm. Magnification: 20x. Images are as follows: (A) DAPI (blue) nuclear stain. (B) Anti-Hsp47 Antibody. (C) Composite... View More
Product Details
MA5-27711
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Human,
Mouse
Host/Isotype
Mouse
/ IgG1, kappa
Class
Monoclonal
Type
Antibody
Clone
1C4-1A6
Immunogen
Human HSP47, full length
Conjugate
Form
Liquid
Concentration
1 mg/mL
Purification
Protein G
Storage buffer
PBS, pH 7.4, with 50% glycerol
Contains
0.09% sodium azide
Storage conditions
-20°C
Shipping conditions
Ambient (domestic); Wet ice (international)
RRID
AB_2735358
Product Specific Information
1 µg/mL of MA5-27711 was sufficient for detection of HSP47 in 20 µg of heat shocked HeLa cell lysate by colorimetric immunoblot analysis using Goat anti-mouse IgG:HRP as the secondary antibody.|Detects 47kDa.
Target Information
SERPINH1 is a member of the serpin superfamily of serine proteinase inhibitors, and its expression is induced by heat shock. The SERPINH1 protein localizes to the endoplasmic reticulum lumen and binds collagen; thus it is thought to be a molecular chaperone involved in the maturation of collagen molecules. Autoantibodies to SERPINH1 have been found in patients with rheumatoid arthritis.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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Bioinformatics
Protein Aliases: 47 kDa heat shock protein; Arsenic-transactivated protein 3; AsTP 3; AsTP3; beta enolase repressor factor 1; CBP 1; CBP 2; Cell proliferation-inducing gene 14 protein; collagen binding protein 1; Collagen-binding protein; Colligen; Colligin; Colligin 1; Colligin 2; colligin-1; colligin-2; HGNC 1547; Hsp 47; PIG 14; RA A47; rheumatoid arthritis antigen A-47; Rheumatoid arthritis-related antigen RA-A47; serine (or cysteine) proteinase inhibitor, clade H (heat shock protein 47), member 1; serine (or cysteine) proteinase inhibitor, clade H (heat shock protein 47), member 1, (collagen binding protein 1); serine (or cysteine) proteinase inhibitor, clade H (heat shock protein 47), member 2; serine (or cysteine) proteinase inhibitor, clade H (heat shock protein 47), member 2, (collagen-binding protein 2); Serine protease inhibitor J6; Serpin H1; serpin peptidase inhibitor, clade H (heat shock protein 47), member 1, (collagen binding protein 1)
Gene Aliases: AsTP3; BERF-1; CBP1; CBP2; gp46; HSP47; J6; OI10; PIG14; PPROM; RA-A47; SERPINH1; SERPINH2
UniProt ID: (Human) P50454, (Mouse) P19324
Entrez Gene ID: (Human) 871, (Mouse) 12406
Performance Guarantee
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