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SETD8 Antibody (PA5-80762) in WB
Western Blot using SETD8 Polyclonal Antibody (Product # PA5-80762) at 1:500 dilution. Lane A: 293 Whole Cell Lysate. Lysates/proteins at 30 μg per lane. Secondary Goat Anti- RabbitIgG H&L (DyLight™ 800) at 1:10,000 dilution. Developed using the Odyssey technique. Performed under reducing conditions. Predicted band size: 43 kDa. Observed band size: 34 kDa. (We are unsure of the identity of these extra bands).
Product Details
PA5-80762
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Human
Host/Isotype
Rabbit
/ IgG
Class
Polyclonal
Type
Antibody
Immunogen
Recombinant Human SETD8/PR-Set7 Protein (Lys195-His352)
Conjugate
Form
Liquid
Purification
Protein A, Antigen affinity chromatography
Storage buffer
PBS
Contains
no preservative
Storage conditions
Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.
Shipping conditions
Ambient (domestic); Wet ice (international)
RRID
AB_2788044
Product Specific Information
This product is preservative free. It is recommended to add sodium azide to avoid contamination (final concentration 0.05%-0.1%).
This antibody has specificity for Human SETD8/PR-Set7.
Target Information
SETD8 is a histone methyltransferase that specifically monomethylates 'Lys-20' of histone H4. H4 'Lys-20' monomethylation is enriched during mitosis and represents a specific tag for epigenetic transcriptional repression. The protein mainly functions in euchromatin regions, thereby playing a central role in the silencing of euchromatic genes. It is required for cell proliferation, probably by contributing to the maintenance of proper higher order structure of DNA during mitosis. It is also involved in chromosome condensation and proper cytokinesis. Nucleosomes are preferred as substrate compared to free histones.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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Bioinformatics
Protein Aliases: H4-K20-HMTase; H4-K20-HMTase KMT5A; H4-K20-HMTase SETD8; H4-K20-specific histone methyltransferase; H4K20-specific histone methyltransferase splice variant Set8b; Histone-lysine N-methyltransferase KMT5A; histone-lysine N-methyltransferase SETD8; lysine (K)-specific methyltransferase 5A; Lysine N-methyltransferase 5A; Lysine-specific methylase 5A; N-lysine methyltransferase KMT5A; N-lysine methyltransferase SETD8; PR-Set7; PR/SET domain containing protein 8; PR/SET domain-containing protein 07; PR/SET07; SET domain containing (lysine methyltransferase) 8; SET domain-containing protein 8
Gene Aliases: KMT5A; PR-Set7; PRSET7; SET07; SET8; SETD8
UniProt ID: (Human) Q9NQR1
Entrez Gene ID: (Human) 387893
negative regulation of transcription from RNA polymerase II promoter
transcription, DNA-templated
mitotic nuclear division
peptidyl-lysine monomethylation
histone H4-K20 methylation
regulation of DNA damage response, signal transduction by p53 class mediator
negative regulation of transcription, DNA-templated
cell division
regulation of signal transduction by p53 class mediator
Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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