Invitrogen

SETDB1 Monoclonal Antibody (5H6A12)

3 Published Figures

4 References

View all (17) SETDB1 antibodies

Datasheet

Protocols

Questions & Answers

Datasheet

Protocols

Questions & Answers

Antibody Testing Data (4)
Published Figures (3)

SETDB1 Antibody in Immunocytochemistry (ICC/IF)

FIGURE: 1 / 7

SETDB1 Antibody (MA5-15722) in ICC/IF

Immunofluorescence analysis of LOVO cells using SETDB1 monoclonal antibody (Product # MA5-15722) (Green). Red: actin filaments have been labeled with phalloidin.

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Product Details

MA5-15722

Applications

Tested Dilution

Publications

Western Blot (WB)

1:500-1:2,000

View 3 publications 3 publications

Immunocytochemistry (ICC/IF)

1:200-1:1,000

ELISA (ELISA)

1:10,000

ChIP assay (ChIP)

View 1 publication 1 publication

Product Specifications

Species Reactivity

Human, Mouse, Non-human primate

Published species

Mouse

Host/Isotype

Mouse / IgG1

Class

Monoclonal

Type

Antibody

Clone

5H6A12

Immunogen

Purified recombinant fragment of human SETDB1 expressed in E. Coli.

Conjugate

Unconjugated Unconjugated Unconjugated

Form

Liquid

Concentration

Conc. not determined

Storage buffer

ascites

Contains

0.03% sodium azide

Storage conditions

Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.

Shipping conditions

Ambient (domestic); Wet ice (international)

AB_10988015

Product Specific Information

MA5-15722 targets SETDB1 in indirect ELISA, IF and WB applications and shows reactivity with Human, mouse, and Non-human primate samples.

The MA5-15722 immunogen is purified recombinant fragment of human SETDB1 expressed in E. Coli.

MA5-15722 detects SETDB1 which has a predicted molecular weight of approximately 180kDa.

Target Information

This gene encodes a histone methyltransferase. The encoded enzyme catalyzes the reaction of S-adenosyl-L-methionine and histone L-lysine to produce S-adenosyl-L-homocysteine and histone N(6)-methyl-L-lysine. The encoded protein likely functions in transcriptional repression. Alternatively spliced transcript variants have been described.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

Product References

Foxd3 controls heterochromatin-mediated repression of repeat elements and 2-cell state transcription.

EMBO reports

Puri D,Koschorz B,Engist B,Onishi-Seebacher M,Ryan D,Soujanya M,Montavon T

Published figure using SETDB1 monoclonal antibody (Product # MA5-15722) in Western Blot

Mon Dec 06 00:00:00 UTC 2021

Complete loss of H3K9 methylation dissolves mouse heterochromatin organization.

Nature communications

Montavon T,Shukeir N,Erikson G,Engist B,Onishi-Seebacher M,Ryan D,Musa Y,Mittler G,Meyer AG,Genoud C,Jenuwein T

MA5-15722 was used in Western Blot to reveal a definitive function for H3K9 methylation in protecting heterochromatin organization and genome integrity.

Fri Jul 16 00:00:00 UTC 2021

The methyltransferase SETDB1 regulates a large neuron-specific topological chromatin domain.

Nature genetics

Jiang Y,Loh YE,Rajarajan P,Hirayama T,Liao W,Kassim BS,Javidfar B,Hartley BJ,Kleofas L,Park RB,Labonte B,Ho SM,Chandrasekaran S,Do C,Ramirez BR,Peter CJ,C W JT,Safaie BM,Morishita H,Roussos P,Nestler EJ,Schaefer A,Tycko B,Brennand KJ,Yagi T,Shen L,Akbarian S

MA5-15722 was used in Chromatin immunoprecipitation to reveal the protective role of methyltransferase Setdb1 in a specific topologically associated domain (TAD): TADcPcdh.

Tue Aug 01 00:00:00 UTC 2017

TRIM28 is an Epigenetic Barrier to Induced Pluripotent Stem Cell Reprogramming.

Stem cells (Dayton, Ohio)

Miles DC,de Vries NA,Gisler S,Lieftink C,Akhtar W,Gogola E,Pawlitzky I,Hulsman D,Tanger E,Koppens M,Beijersbergen RL,van Lohuizen M

MA5-15722 was used in western blot to characterize TRIM28 as an epigenetic barrier to generate reprogramming of pluripotent stem cells

Sun Jan 01 00:00:00 UTC 2017

Bioinformatics

Protein Aliases: ERG-associated protein with a SET domain, ESET; ERG-associated protein with SET domain; ESET; H3-K9-HMTase 4; Histone H3-K9 methyltransferase 4; Histone-lysine N-methyltransferase SETDB1; histone-lysine N-methyltransferase, H3lysine-9 specific 4; Lysine N-methyltransferase 1E; SET domain bifurcated 1; SET domain ERG-associated histone methyltransferase; SET domain, bifurcated 1; tudor domain containing 21

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Gene Aliases: AU022152; ESET; H3-K9-HMTase4; KG1T; KIAA0067; KMT1E; mKIAA0067; SETDB1; TDRD21

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UniProt ID: (Human) Q15047

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Entrez Gene ID: (Human) 9869, (Mouse) 84505

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Function(s)

DNA binding chromatin binding protein binding zinc ion binding histone-lysine N-methyltransferase activity promoter-specific chromatin binding methyltransferase activity transferase activity metal ion binding

Process(es)

negative regulation of transcription from RNA polymerase II promoter inner cell mass cell proliferation transcription, DNA-templated regulation of transcription, DNA-templated chromatin modification methylation histone lysine methylation histone H3-K9 trimethylation bone development Ras protein signal transduction response to vitamin response to ethanol positive regulation of methylation-dependent chromatin silencing

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SETDB1 Monoclonal Antibody (5H6A12)

SETDB1 Antibody (MA5-15722) in ICC/IF

Product Details

MA5-15722

Western Blot (WB)

Immunocytochemistry (ICC/IF)

ELISA (ELISA)

ChIP assay (ChIP)

Species Reactivity

Published species

Host/Isotype

Class

Type

Clone

Immunogen

Conjugate

Form

Concentration

Storage buffer

Contains

Storage conditions

Shipping conditions

RRID

Product Specific Information

Target Information

Bioinformatics

Performance Guarantee

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