Proteintech

SF3B3 Polyclonal Antibody

SF3B3 Antibody in Immunocytochemistry (ICC/IF)

FIGURE: 1 / 16

SF3B3 Antibody (14577-1-AP) in ICC/IF

Immunofluorescent analysis of Hela cells, using SF3B3 antibody 14577-1-AP at 1:50 dilution and Rhodamine-labeled goat anti-rabbit IGG (red). Blue pseudocolor = DAPI (fluorescent DNA dye).

SF3B3 Antibody in Immunohistochemistry (Paraffin) (IHC (P))

SF3B3 Antibody in Immunoprecipitation (IP)

Product Image Gallery

Product Details

14577-1-AP

Applications

Tested Dilution

Publications

Western Blot (WB)

1:1,000-1:6,000

View 13 publications 13 publications

Immunohistochemistry (Paraffin) (IHC (P))

1:20-1:200

Immunocytochemistry (ICC/IF)

1:20-1:200

View 2 publications 2 publications

Immunoprecipitation (IP)

0.5-4.0 µg

View 1 publication 1 publication

RNA Immunoprecipitation (RIP)

View 1 publication 1 publication

Miscellaneous PubMed (MISC)

View 1 publication 1 publication

Not applicable (N/A)

View 1 publication 1 publication

Product Specifications

Species Reactivity

Human, Mouse, Rat

Published species

Human

Host/Isotype

Rabbit / IgG

Class

Polyclonal

Type

Antibody

Immunogen

SF3B3 Fusion Protein Ag5980 (250-399 aa encoded by BC003146)

View immunogen

Conjugate

Unconjugated Unconjugated Unconjugated

Form

Liquid

Concentration

0.32 mg/mL

Purification

Antigen affinity chromatography

Storage buffer

PBS, pH 7.3, with 50% glycerol

Contains

0.02% sodium azide

Storage conditions

-20°C

Shipping conditions

Wet ice (domestic); Dry ice (international)

Product Specific Information

Immunogen sequence: EDPTGNKAL WDRGLLNGAS QKAEVIMNYH VGETVLSLQK TTLIPGGSES LVYTTLSGGI GILVPFTSHE DHDFFQHVEM HLRSEHPPLC GRDHLSFRSY YFPVKNVIDG DLCEQFNSME PNKQKNVSEE LDRTPPEVSK KLEDIRTRYA F (250-399 aa encoded by BC003146)

Target Information

This gene encodes subunit 3 of the splicing factor 3b protein complex. Splicing factor 3b, together with splicing factor 3a and a 12S RNA unit, forms the U2 small nuclear ribonucleoproteins complex transcription coactivator-HAT complex, and the TFTC -containing complex). These complexes may function in chromatin modification, transcription, splicing, and DNA repair.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

Product References

Splicing factor SF3B3, a NS5-binding protein, restricts ZIKV infection by targeting GCH1.

Virologica Sinica

Chen T,Yang H,Liu P,Hamiti M,Zhang X,Xu Y,Quan W,Zhang Y,Yu W,Jiao L,Du T,Xi J,Yin B,Zhou W,Lu S,Peng X

Sat Apr 01 00:00:00 UTC 2023

Splicing factor SF3B3, a NS5-binding protein, restricts ZIKV infection by targeting GCH1.

Virologica Sinica

Chen T,Yang H,Liu P,Hamiti M,Zhang X,Xu Y,Quan W,Zhang Y,Yu W,Jiao L,Du T,Xi J,Yin B,Zhou W,Lu S,Peng X

Sat Apr 01 00:00:00 UTC 2023

Depleted HDAC3 attenuates hyperuricemia-induced renal interstitial fibrosis via miR-19b-3p/SF3B3 axis.

Cell cycle (Georgetown, Tex.)

Hu L,Yang K,Mai X,Wei J,Ma C

Tue Mar 01 00:00:00 UTC 2022

Key pituitary miRNAs mediate the expression of pig GHRHR splice variants by regulating splice factors.

International journal of biological macromolecules

Teng Z,Hao L,Yang R,Song J,Wang Z,Jiao Y,Fang J,Zheng S,Ma Z,Chen X,Liu S,Cheng Y

Tue May 31 00:00:00 UTC 2022

CircSCAP interacts with SF3A3 to inhibit the malignance of non-small cell lung cancer by activating p53 signaling.

Journal of experimental & clinical cancer research : CR

Chen D,Zhou H,Cai Z,Cai K,Liu J,Wang W,Miao H,Li H,Li R,Li X,Chen Y,Wang HY,Wen Z

Fri Apr 01 00:00:00 UTC 2022

Multi-omics approach reveals posttranscriptionally regulated genes are essential for human pluripotent stem cells.

iScience

Iwasaki M,Kawahara Y,Okubo C,Yamakawa T,Nakamura M,Tabata T,Nishi Y,Narita M,Ohta A,Saito H,Yamamoto T,Nakagawa M,Yamanaka S,Takahashi K

Fri May 20 00:00:00 UTC 2022

Nuclear speckle specific hnRNP D-like prevents age- and AD-related cognitive decline by modulating RNA splicing.

Molecular neurodegeneration

Zhang Q,Zhang J,Ye J,Li X,Liu H,Ma X,Wang C,He K,Zhang W,Yuan J,Zhao Y,Xu H,Liu Q

Wed Sep 22 00:00:00 UTC 2021

Corrigendum to article "Quantitative proteomics reveals that long non-coding RNA MALAT1 interacts with DBC1 to regulate p53 acetylation''.

Nucleic acids research

Chen R,Liu Y,Zhuang H,Yang B,Hei K,Xiao M,Hou C,Gao H,Zhang X,Jia C,Li L,Li Y,Zhang N

Mon Apr 19 00:00:00 UTC 2021

Interactome analysis illustrates diverse gene regulatory processes associated with LIN28A in human iPS cell-derived neural progenitor cells.

iScience

Yu NK,McClatchy DB,Diedrich JK,Romero S,Choi JH,Martínez-Bartolomé S,Delahunty CM,Muotri AR,Yates JR

Fri Nov 19 00:00:00 UTC 2021

Interactome analysis illustrates diverse gene regulatory processes associated with LIN28A in human iPS cell-derived neural progenitor cells.

iScience

Yu NK,McClatchy DB,Diedrich JK,Romero S,Choi JH,Martínez-Bartolomé S,Delahunty CM,Muotri AR,Yates JR

Fri Nov 19 00:00:00 UTC 2021

Enterotoxigenic Bacteroidesfragilis Promotes Intestinal Inflammation and Malignancy by Inhibiting Exosome-Packaged miR-149-3p.

Gastroenterology

Cao Y,Wang Z,Yan Y,Ji L,He J,Xuan B,Shen C,Ma Y,Jiang S,Ma D,Tong T,Zhang X,Gao Z,Zhu X,Fang JY,Chen H,Hong J

Mon Nov 01 00:00:00 UTC 2021

PHF5A Epigenetically Inhibits Apoptosis to Promote Breast Cancer Progression.

Cancer research

Zheng YZ,Xue MZ,Shen HJ,Li XG,Ma D,Gong Y,Liu YR,Qiao F,Xie HY,Lian B,Sun WL,Zhao HY,Yao L,Zuo WJ,Li DQ,Wang P,Hu X,Shao ZM

Fri Jun 15 00:00:00 UTC 2018

PHF5A Epigenetically Inhibits Apoptosis to Promote Breast Cancer Progression.

Cancer research

Zheng YZ,Xue MZ,Shen HJ,Li XG,Ma D,Gong Y,Liu YR,Qiao F,Xie HY,Lian B,Sun WL,Zhao HY,Yao L,Zuo WJ,Li DQ,Wang P,Hu X,Shao ZM

Fri Jun 15 00:00:00 UTC 2018

Quantitative proteomics reveals that long non-coding RNA MALAT1 interacts with DBC1 to regulate p53 acetylation.

Nucleic acids research

Chen R,Liu Y,Zhuang H,Yang B,Hei K,Xiao M,Hou C,Gao H,Zhang X,Jia C,Li L,Li Y,Zhang N

Fri Sep 29 00:00:00 UTC 2017

Quantitative proteomics reveals that long non-coding RNA MALAT1 interacts with DBC1 to regulate p53 acetylation.

Nucleic acids research

Chen R,Liu Y,Zhuang H,Yang B,Hei K,Xiao M,Hou C,Gao H,Zhang X,Jia C,Li L,Li Y,Zhang N

Fri Sep 29 00:00:00 UTC 2017

Splicing modulators act at the branch point adenosine binding pocket defined by the PHF5A-SF3b complex.

Nature communications

Teng T,Tsai JH,Puyang X,Seiler M,Peng S,Prajapati S,Aird D,Buonamici S,Caleb B,Chan B,Corson L,Feala J,Fekkes P,Gerard B,Karr C,Korpal M,Liu X,T Lowe J,Mizui Y,Palacino J,Park E,Smith PG,Subramanian V,Wu ZJ,Zou J,Yu L,Chicas A,Warmuth M,Larsen N,Zhu P

Thu May 25 00:00:00 UTC 2017

RNA structure replaces the need for U2AF2 in splicing.

Genome research

Lin CL,Taggart AJ,Lim KH,Cygan KJ,Ferraris L,Creton R,Huang YT,Fairbrother WG

Fri Jan 01 00:00:00 UTC 2016

RNA structure replaces the need for U2AF2 in splicing.

Genome research

Lin CL,Taggart AJ,Lim KH,Cygan KJ,Ferraris L,Creton R,Huang YT,Fairbrother WG

Fri Jan 01 00:00:00 UTC 2016

SF3B1 association with chromatin determines splicing outcomes.

Cell reports

Kfir N,Lev-Maor G,Glaich O,Alajem A,Datta A,Sze SK,Meshorer E,Ast G

Tue Apr 28 00:00:00 UTC 2015

SF3B1 association with chromatin determines splicing outcomes.

Cell reports

Kfir N,Lev-Maor G,Glaich O,Alajem A,Datta A,Sze SK,Meshorer E,Ast G

Tue Apr 28 00:00:00 UTC 2015

Rearrangements within human spliceosomes captured after exon ligation.

RNA (New York, N.Y.)

Ilagan JO,Chalkley RJ,Burlingame AL,Jurica MS

Fri Mar 01 00:00:00 UTC 2013

Rearrangements within human spliceosomes captured after exon ligation.

RNA (New York, N.Y.)

Ilagan JO,Chalkley RJ,Burlingame AL,Jurica MS

Fri Mar 01 00:00:00 UTC 2013

Breaking up the C complex spliceosome shows stable association of proteins with the lariat intron intermediate.

PloS one

Coltri P,Effenberger K,Chalkley RJ,Burlingame AL,Jurica MS

Tue Apr 19 00:00:00 UTC 2011

Breaking up the C complex spliceosome shows stable association of proteins with the lariat intron intermediate.

PloS one

Coltri P,Effenberger K,Chalkley RJ,Burlingame AL,Jurica MS

Tue Apr 19 00:00:00 UTC 2011

Bioinformatics

Protein Aliases: pre-mRNA splicing factor SF3b, 130 kDa subunit; Pre-mRNA-splicing factor SF3b 130 kDa subunit; SAP 130; SF3b130; Spliceosome-associated protein 130; Splicing factor 3B subunit 3; STAF130

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Gene Aliases: 1810061H24Rik; 5730409A01Rik; AA409318; D8Ertd633e; KIAA0017; mKIAA0017; RSE1; SAP130; SF3b130; SF3B3; STAF130

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UniProt ID: (Human) Q15393, (Mouse) Q921M3

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Entrez Gene ID: (Human) 23450, (Rat) 292019, (Mouse) 101943

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Function(s)

RNA binding protein binding molecular_function nucleic acid binding

Process(es)

RNA splicing, via transesterification reactions mRNA splicing, via spliceosome mRNA processing protein complex assembly RNA splicing negative regulation of protein catabolic process

Performance Guarantee

If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*

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SF3B3 Polyclonal Antibody

SF3B3 Antibody (14577-1-AP) in ICC/IF

Product Details

14577-1-AP

Western Blot (WB)

Immunohistochemistry (Paraffin) (IHC (P))

Immunocytochemistry (ICC/IF)

Immunoprecipitation (IP)

RNA Immunoprecipitation (RIP)

Miscellaneous PubMed (MISC)

Not applicable (N/A)

Species Reactivity

Published species

Host/Isotype

Class

Type

Immunogen

Conjugate

Form

Concentration

Purification

Storage buffer

Contains

Storage conditions

Shipping conditions

Product Specific Information

Target Information

Bioinformatics

Performance Guarantee

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