Proteintech

SFPQ Polyclonal Antibody

SFPQ Antibody in Immunocytochemistry (ICC/IF)

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SFPQ Antibody (15585-1-AP) in ICC/IF

Immunofluorescent analysis of ( 10% Formaldehyde ) fixed HeLa cells using 15585-1-AP (SFPQ antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IGG (H+L).

SFPQ Antibody in Immunohistochemistry (Paraffin) (IHC (P))

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Product Details

15585-1-AP

Applications

Tested Dilution

Publications

Western Blot (WB)

1:500-1:3,000

View 13 publications 13 publications

Immunohistochemistry (IHC)

View 1 publication 1 publication

Immunohistochemistry (Paraffin) (IHC (P))

1:20-1:200

Immunocytochemistry (ICC/IF)

1:50-1:500

View 5 publications 5 publications

Immunoprecipitation (IP)

View 1 publication 1 publication

RNA Immunoprecipitation (RIP)

View 3 publications 3 publications

Not applicable (N/A)

View 1 publication 1 publication

Product Specifications

Species Reactivity

Human, Mouse, Rat

Published species

Human, Mouse

Host/Isotype

Rabbit / IgG

Class

Polyclonal

Type

Antibody

Immunogen

SFPQ Fusion Protein Ag7181 (1-300 aa encoded by BC051192)

View immunogen

Conjugate

Unconjugated Unconjugated Unconjugated

Form

Liquid

Concentration

0.19 mg/mL

Purification

Antigen affinity chromatography

Storage buffer

PBS, pH 7.3, with 50% glycerol

Contains

0.02% sodium azide

Storage conditions

-20°C

Shipping conditions

Wet ice (domestic); Dry ice (international)

Product Specific Information

Immunogen sequence: MSRDRFRSR GGGGGGFHRR GGGGGRGGLH DFRSPPPGMG LNQNRGPMGP GPGQSGPKPP IPPPPPHQQQ QQPPPQQPPP QQPPPHQPPP HPQPHQQQQP PPPPQDSSKP VVAQGPGPAP GVGSTPPASS SAPPATPPTS GAPPGSGPGP TPTPPPAVTS APPGAPPPTP PSSGVPTTPP QAGGPPPPPA AVPGPGPGPK QGPGPGGPKG GKMPGGPKPG GGPGLSTPGG HPKPPRRGGG EPRGGRQHHP PYHQQHHQGP PPGGPGGRSE EKISDSEGFK ANLSLLRRPG EKTYTQRCRL F (1-300 aa encoded by BC051192)

Target Information

SFPQ, also named PSF, encodes a nuclear factor implicated in the splicing and regulation of gene expression. SFPQ probably forms a heteromer with NONO and participates in DNA pairing and DNA break repair program. Very recently SFPQ was identified as a downstream target of tau, complete nuclear depletion and cytoplasmic accumulation of SFPQ were shown in the neurons and astrocytes of brains with Alzheimer's disease (AD), more strikingly, reduced SFPQ levels may progress together with tau pathology, these observation strongly suggests the important role of SFPQ pathology in neurodegenerative diseases including AD. SFPQ protein may appears as 47 kDa, 52 kDa or 100 kDa molecules in various cell types.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

Product References

K235 acetylation couples with PSPC1 to regulate the m⁶A demethylation activity of ALKBH5 and tumorigenesis.

Nature communications

Zhang XL,Chen XH,Xu B,Chen M,Zhu S,Meng N,Wang JZ,Zhu H,Chen D,Liu JB,Yan GR

Tue Jun 27 00:00:00 UTC 2023

Decreased LONP1 expression contributes to DNA damage and meiotic defects in oocytes.

Molecular reproduction and development

Liu C,Xu M,Guan Y,Li L,Liu W,Guo B,Sheng X,Zhang Y,Zhou J,Zhen X,Yan G,Sun H,Ding L

Thu Jun 01 00:00:00 UTC 2023

Decreased LONP1 expression contributes to DNA damage and meiotic defects in oocytes.

Molecular reproduction and development

Liu C,Xu M,Guan Y,Li L,Liu W,Guo B,Sheng X,Zhang Y,Zhou J,Zhen X,Yan G,Sun H,Ding L

Thu Jun 01 00:00:00 UTC 2023

Targeting the splicing factor NONO inhibits GBM progression through GPX1 intron retention.

Theranostics

Wang X,Han M,Wang S,Sun Y,Zhao W,Xue Z,Liang X,Huang B,Li G,Chen A,Li X,Wang J

Tue Aug 02 00:00:00 UTC 2022

MALAT1 modulates alternative splicing by cooperating with the splicing factors PTBP1 and PSF.

Science advances

Miao H,Wu F,Li Y,Qin C,Zhao Y,Xie M,Dai H,Yao H,Cai H,Wang Q,Song X,Li L

Fri Dec 23 00:00:00 UTC 2022

MALAT1 modulates alternative splicing by cooperating with the splicing factors PTBP1 and PSF.

Science advances

Miao H,Wu F,Li Y,Qin C,Zhao Y,Xie M,Dai H,Yao H,Cai H,Wang Q,Song X,Li L

Fri Dec 23 00:00:00 UTC 2022

Comparison of viral RNA-host protein interactomes across pathogenic RNA viruses informs rapid antiviral drug discovery for SARS-CoV-2.

Cell research

Zhang S,Huang W,Ren L,Ju X,Gong M,Rao J,Sun L,Li P,Ding Q,Wang J,Zhang QC

Sat Jan 01 00:00:00 UTC 2022

LncRNA NEAT1 Potentiates SREBP2 Activity to Promote Inflammatory Macrophage Activation and Limit Hantaan Virus Propagation.

Frontiers in microbiology

Yang Y,Li M,Ma Y,Ye W,Si Y,Zheng X,Liu H,Cheng L,Zhang L,Zhang H,Zhang X,Lei Y,Shen L,Zhang F,Ma H

Sat Jul 16 00:00:00 UTC 2022

RNA binding protein NKAP protects glioblastoma cells from ferroptosis by promoting SLC7A11 mRNA splicing in an m⁶A-dependent manner.

Cell death & disease

Sun S,Gao T,Pang B,Su X,Guo C,Zhang R,Pang Q

Fri Jan 21 00:00:00 UTC 2022

RNA binding protein NKAP protects glioblastoma cells from ferroptosis by promoting SLC7A11 mRNA splicing in an m⁶A-dependent manner.

Cell death & disease

Sun S,Gao T,Pang B,Su X,Guo C,Zhang R,Pang Q

Fri Jan 21 00:00:00 UTC 2022

Genome-wide analysis of pseudogenes reveals HBBP1's human-specific essentiality in erythropoiesis and implication in β-thalassemia.

Developmental cell

Ma Y,Liu S,Gao J,Chen C,Zhang X,Yuan H,Chen Z,Yin X,Sun C,Mao Y,Zhou F,Shao Y,Liu Q,Xu J,Cheng L,Yu D,Li P,Yi P,He J,Geng G,Guo Q,Si Y,Zhao H,Li H,Banes GL,Liu H,Nakamura Y,Kurita R,Huang Y,Wang X,Wang F,Fang G,Engel JD,Shi L,Zhang YE,Yu J

Mon Feb 22 00:00:00 UTC 2021

LncRNA nuclear-enriched abundant transcript 1 shuttled by prostate cancer cells-secreted exosomes initiates osteoblastic phenotypes in the bone metastatic microenvironment via miR-205-5p/runt-related transcription factor 2/splicing factor proline- and glutamine-rich/polypyrimidine tract-binding protein 2 axis.

Clinical and translational medicine

Mo C,Huang B,Zhuang J,Jiang S,Guo S,Mao X

Sun Aug 01 00:00:00 UTC 2021

The tumor-suppressive effects of alpha-ketoglutarate-dependent dioxygenase FTO via N6-methyladenosine RNA methylation on bladder cancer patients.

Bioengineered

Yi W,Yu Y,Li Y,Yang J,Gao S,Xu L

Wed Dec 01 00:00:00 UTC 2021

AR-induced long non-coding RNA LINC01503 facilitates proliferation and metastasis via the SFPQ-FOSL1 axis in nasopharyngeal carcinoma.

Oncogene

He SW,Xu C,Li YQ,Li YQ,Zhao Y,Zhang PP,Lei Y,Liang YL,Li JY,Li Q,Chen Y,Huang SY,Ma J,Liu N

Sat Aug 01 00:00:00 UTC 2020

AR-induced long non-coding RNA LINC01503 facilitates proliferation and metastasis via the SFPQ-FOSL1 axis in nasopharyngeal carcinoma.

Oncogene

He SW,Xu C,Li YQ,Li YQ,Zhao Y,Zhang PP,Lei Y,Liang YL,Li JY,Li Q,Chen Y,Huang SY,Ma J,Liu N

Sat Aug 01 00:00:00 UTC 2020

AR-induced long non-coding RNA LINC01503 facilitates proliferation and metastasis via the SFPQ-FOSL1 axis in nasopharyngeal carcinoma.

Oncogene

He SW,Xu C,Li YQ,Li YQ,Zhao Y,Zhang PP,Lei Y,Liang YL,Li JY,Li Q,Chen Y,Huang SY,Ma J,Liu N

Sat Aug 01 00:00:00 UTC 2020

Splicing Regulator p54^nrb /Non-POU Domain-Containing Octamer-Binding Protein Enhances Carcinogenesis Through Oncogenic Isoform Switch of MYC Box-Dependent Interacting Protein 1 in Hepatocellular Carcinoma.

Hepatology (Baltimore, Md.)

Hu Z,Dong L,Li S,Li Z,Qiao Y,Li Y,Ding J,Chen Z,Wu Y,Wang Z,Huang S,Gao Q,Zhao Y,He X

Sat Aug 01 00:00:00 UTC 2020

Characterization of telomeric repeat-containing RNA (TERRA) localization and protein interactions in primordial germ cells of the mouse†.

Biology of reproduction

Brieño-Enríquez MA,Moak SL,Abud-Flores A,Cohen PE

Mon Apr 01 00:00:00 UTC 2019

Genome-wide CRISPR screen identifies ELP5 as a determinant of gemcitabine sensitivity in gallbladder cancer.

Nature communications

Xu S,Zhan M,Jiang C,He M,Yang L,Shen H,Huang S,Huang X,Lin R,Shi Y,Liu Q,Chen W,Mohan M,Wang J

Mon Dec 02 00:00:00 UTC 2019

The lncRNA Neat1 promotes activation of inflammasomes in macrophages.

Nature communications

Zhang P,Cao L,Zhou R,Yang X,Wu M

Tue Apr 02 00:00:00 UTC 2019

The Long Noncoding RNA NEAT1 Exerts Antihantaviral Effects by Acting as Positive Feedback for RIG-I Signaling.

Journal of virology

Ma H,Han P,Ye W,Chen H,Zheng X,Cheng L,Zhang L,Yu L,Wu X,Xu Z,Lei Y,Zhang F

Mon May 01 00:00:00 UTC 2017

The Long Noncoding RNA NEAT1 Exerts Antihantaviral Effects by Acting as Positive Feedback for RIG-I Signaling.

Journal of virology

Ma H,Han P,Ye W,Chen H,Zheng X,Cheng L,Zhang L,Yu L,Wu X,Xu Z,Lei Y,Zhang F

Mon May 01 00:00:00 UTC 2017

Synergistic upregulation of NONO and PSPC1 regulates Sertoli cell response to MEHP via modulation of ALDH1A1 signaling.

FEBS letters

Dong BW,Jin XH,Yan CY,Yang T,Cai GQ,Lu J

Wed Mar 01 00:00:00 UTC 2017

Synergistic upregulation of NONO and PSPC1 regulates Sertoli cell response to MEHP via modulation of ALDH1A1 signaling.

FEBS letters

Dong BW,Jin XH,Yan CY,Yang T,Cai GQ,Lu J

Wed Mar 01 00:00:00 UTC 2017

Proteins implicated in the increase of adhesivity induced by suberoylanilide hydroxamic acid in leukemic cells.

Journal of proteomics

Grebeňová D,Röselová P,Pluskalová M,Halada P,Rösel D,Suttnar J,Brodská B,Otevřelová P,Kuželová K

Fri Dec 21 00:00:00 UTC 2012

Proteins implicated in the increase of adhesivity induced by suberoylanilide hydroxamic acid in leukemic cells.

Journal of proteomics

Grebeňová D,Röselová P,Pluskalová M,Halada P,Rösel D,Suttnar J,Brodská B,Otevřelová P,Kuželová K

Fri Dec 21 00:00:00 UTC 2012

New insights into the functions and localization of nuclear CCT protein complex in K562 leukemia cells.

Proteomics. Clinical applications

Huang R,Yu M,Li CY,Zhan YQ,Xu WX,Xu F,Ge CH,Li W,Yang XM

Mon Oct 01 00:00:00 UTC 2012

New insights into the functions and localization of nuclear CCT protein complex in K562 leukemia cells.

Proteomics. Clinical applications

Huang R,Yu M,Li CY,Zhan YQ,Xu WX,Xu F,Ge CH,Li W,Yang XM

Mon Oct 01 00:00:00 UTC 2012

Bioinformatics

Protein Aliases: 100 kDa DNA-pairing protein; DNA-binding p52/p100 complex, 100 kDa subunit; hPOMp100; NonO/p54nrb homolog; polypyrimidine tract binding protein associated; polypyrimidine tract-binding protein-associated splicing factor; Polypyrimidine tract-binding protein-associated-splicing factor; protein phosphatase 1, regulatory subunit 140; PSF; PTB-associated splicing factor; PTB-associated-splicing factor; splicing factor proline/glutamine rich (polypyrimidine tract binding protein associated); splicing factor proline/glutamine rich (polypyrimidine tract-binding protein-associated); splicing factor proline/glutamine-rich; Splicing factor, proline- and glutamine-rich

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Gene Aliases: 1110004P21Rik; 2810416M14Rik; 5730453G22Rik; 9030402K04Rik; AU021830; D4Ertd314e; Gm12940; OTTMUSG00000009329; POMP100; PPP1R140; PSF; REP1; SFPQ

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UniProt ID: (Human) P23246, (Mouse) Q8VIJ6

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Entrez Gene ID: (Human) 6421, (Mouse) 71514, (Rat) 252855

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Function(s)

nucleotide binding transcription regulatory region sequence-specific DNA binding RNA polymerase II distal enhancer sequence-specific DNA binding core promoter binding chromatin binding protein binding histone deacetylase binding transcription regulatory region DNA binding poly(A) RNA binding nucleic acid binding DNA binding RNA binding

Process(es)

negative regulation of transcription from RNA polymerase II promoter alternative mRNA splicing, via spliceosome double-strand break repair via homologous recombination DNA repair transcription, DNA-templated regulation of transcription, DNA-templated mRNA processing cellular response to DNA damage stimulus RNA splicing regulation of circadian rhythm negative regulation of circadian rhythm positive regulation of sister chromatid cohesion negative regulation of transcription, DNA-templated rhythmic process chromosome organization regulation of cell cycle histone H3 deacetylation positive regulation of oxidative stress-induced intrinsic apoptotic signaling pathway

Performance Guarantee

If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*

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SFPQ Polyclonal Antibody

SFPQ Antibody (15585-1-AP) in ICC/IF

Product Details

15585-1-AP

Western Blot (WB)

Immunohistochemistry (IHC)

Immunohistochemistry (Paraffin) (IHC (P))

Immunocytochemistry (ICC/IF)

Immunoprecipitation (IP)

RNA Immunoprecipitation (RIP)

Not applicable (N/A)

Species Reactivity

Published species

Host/Isotype

Class

Type

Immunogen

Conjugate

Form

Concentration

Purification

Storage buffer

Contains

Storage conditions

Shipping conditions

Product Specific Information

Target Information

Bioinformatics

Performance Guarantee

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