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          • Primary Antibodies ›
          • SFRP1 Antibodies

          Invitrogen

          SFRP1 Recombinant Rabbit Monoclonal Antibody (JA11-68)

          Advanced Verification
          View all (18) SFRP1 antibodies

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          Datasheet
          Protocols
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          Datasheet
          Protocols
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          Cite SFRP1 Recombinant Rabbit Monoclonal Antibody (JA11-68)

          • Antibody Testing Data (6)
          • Advanced Verification (4)
          SFRP1 Antibody in Immunocytochemistry (ICC/IF)
          Group 53 Created with Sketch.
          SFRP1 Antibody in Immunocytochemistry (ICC/IF)
          Group 53 Created with Sketch.

          FIGURE: 1 / 10

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          SFRP1 Antibody (MA5-32675) in ICC/IF

          Immunofluorescence analysis of SFRP1 was performed using 70% confluent log phase MCF 10A and MCF7 cells untreated as well as PTI treated. The cells were fixed with 4% paraformaldehyde for 10 minutes, permeabilized with 0.1% Triton™ X-100 for 15 minutes, and blocked with 2% BSA for 45 minutes at room temperature. The cells were labeled with SFRP1 Recombinant Rabbit Monoclonal Antibody (JA11-68) (Product # MA5-32675) at 1:100 dilution in 0.1% BSA, incubated at 4 degree Celsius overnight and then labeled with Donkey anti-Rabbit IgG (H+L) High... View More {{ $ctrl.currentElement.advancedVerification.fullName }} validation info. View more
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          View Product

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          SFRP1 Antibody in Immunocytochemistry (ICC/IF)
          SFRP1 Antibody in Western Blot (WB)
          SFRP1 Antibody in Western Blot (WB)
          SFRP1 Antibody in Western Blot (WB)
          SFRP1 Antibody in Western Blot (WB)
          SFRP1 Antibody in Immunoprecipitation (IP)
          SFRP1 Antibody
          SFRP1 Antibody
          SFRP1 Antibody
          SFRP1 Antibody

          Product Details

          MA5-32675

          Applications
          Tested Dilution
          Publications

          Western Blot (WB)

          1:500-1:2,000
          -

          Immunohistochemistry (Paraffin) (IHC (P))

          1:1,000
          -

          Immunocytochemistry (ICC/IF)

          1:100
          -

          Immunoprecipitation (IP)

          Assay-dependent
          -
          Product Specifications

          Species Reactivity

          Human, Mouse

          Host/Isotype

          Rabbit / IgG

          Expression System

          HEK293 cells

          Class

          Recombinant Monoclonal

          Type

          Antibody

          Clone

          JA11-68

          Immunogen

          Synthetic peptide within Human SFRP1 aa 31-80
          View immunogen

          Conjugate

          Unconjugated Unconjugated Unconjugated

          Form

          Liquid

          Concentration

          1 mg/mL

          Purification

          Protein A

          Storage buffer

          TBS, pH 7.4, with 40% Glycerol, 0.05% BSA

          Contains

          0.05% sodium azide

          Storage conditions

          Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.

          Shipping conditions

          Ambient (domestic); Wet ice (international)

          RRID

          AB_2809952

          Product Specific Information

          Recombinant rabbit monoclonal antibodies are produced using in vitro expression systems. The expression systems are developed by cloning in the specific antibody DNA sequences from immunoreactive rabbits. Then, individual clones are screened to select the best candidates for production. The advantages of using recombinant rabbit monoclonal antibodies include: better specificity and sensitivity, lot-to-lot consistency, animal origin-free formulations, and broader immunoreactivity to diverse targets due to larger rabbit immune repertoire.

          Target Information

          Secreted frizzled-related protein 1 (SFRP1) is a member of the SFRP family that contains a cysteine-rich domain homologous to the putative Wnt-binding site of Frizzled proteins. SFRPs act as soluble modulators of Wnt signaling. SFRP1 and SFRP5 may be involved in determining the polarity of photoreceptor cells in the retina. SFRP1 is expressed in several human tissues, with the highest levels in heart.

          For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

          References (0)

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          Cite this product

          Bioinformatics

          Protein Aliases: frizzled-related protein; FRP-1; SARP-2; Secreted apoptosis-related protein 2; Secreted frizzled-related protein 1; secreted frizzled-related sequence protein 1; sFRP-1

          View more View less

          Gene Aliases: 2210415K03Rik; AW011917; AW107218; AW742929; FRP; FRP-1; FRP1; FrzA; SARP2; sFRP-1; SFRP1

          View more View less

          UniProt ID: (Human) Q8N474, (Mouse) Q8C4U3

          View more View less

          Entrez Gene ID: (Human) 6422, (Mouse) 20377

          View more View less

          Function(s)
          cysteine-type endopeptidase activity G-protein coupled receptor activity frizzled binding protein binding drug binding heparin binding Wnt-protein binding identical protein binding Wnt-activated receptor activity
          Process(es)
          somitogenesis neural tube closure hematopoietic progenitor cell differentiation proteolysis multicellular organism development positive regulation of cell proliferation negative regulation of cell proliferation male gonad development female gonad development dorsal/ventral axis specification anterior/posterior pattern specification regulation of cell cycle process negative regulation of gene expression negative regulation of epithelial to mesenchymal transition neural crest cell fate commitment response to organic cyclic compound Wnt signaling pathway neural tube development actin cytoskeleton organization hemopoiesis cell differentiation positive regulation of Wnt signaling pathway negative regulation of Wnt signaling pathway regulation of ossification negative regulation of ossification positive regulation of cell growth negative regulation of cell growth negative regulation of cell migration negative regulation of BMP signaling pathway negative regulation of osteoblast proliferation substrate adhesion-dependent cell spreading somatic stem cell population maintenance response to drug positive regulation of apoptotic process negative regulation of apoptotic process negative regulation of JUN kinase activity cellular response to fibroblast growth factor stimulus negative regulation of B cell differentiation positive regulation of fat cell differentiation negative regulation of osteoblast differentiation negative regulation of osteoclast differentiation positive regulation of smoothened signaling pathway negative regulation of transcription, DNA-templated positive regulation of transcription, DNA-templated development of primary male sexual characteristics negative regulation of insulin secretion negative regulation of bone remodeling negative regulation of fibroblast proliferation digestive tract morphogenesis positive regulation of epithelial cell proliferation negative regulation of epithelial cell proliferation negative regulation of peptidyl-tyrosine phosphorylation canonical Wnt signaling pathway Wnt signaling pathway, planar cell polarity pathway hematopoietic stem cell differentiation bone trabecula formation prostate epithelial cord arborization involved in prostate glandular acinus morphogenesis regulation of branching involved in prostate gland morphogenesis negative regulation of androgen receptor signaling pathway cellular response to tumor necrosis factor cellular response to growth factor stimulus cellular response to estrogen stimulus cellular response to X-ray cellular response to heparin negative regulation of canonical Wnt signaling pathway regulation of establishment of planar polarity planar cell polarity pathway involved in neural tube closure Wnt signaling pathway involved in somitogenesis convergent extension involved in somitogenesis positive regulation of canonical Wnt signaling pathway positive regulation of extrinsic apoptotic signaling pathway via death domain receptors negative regulation of planar cell polarity pathway involved in axis elongation positive regulation of non-canonical Wnt signaling pathway negative regulation of Wnt signaling pathway involved in dorsal/ventral axis specification negative regulation of canonical Wnt signaling pathway involved in controlling type B pancreatic cell proliferation negative regulation of fibroblast apoptotic process positive regulation of fibroblast apoptotic process positive regulation of extrinsic apoptotic signaling pathway osteoblast differentiation ureteric bud development positive regulation of cell-matrix adhesion G-protein coupled receptor signaling pathway cellular response to starvation menstrual cycle phase positive regulation of GTPase activity stromal-epithelial cell signaling involved in prostate gland development regulation of angiogenesis positive regulation of stress fiber assembly positive regulation of focal adhesion assembly cellular response to vitamin D cellular response to interleukin-1 cellular response to prostaglandin E stimulus cellular response to estradiol stimulus cellular response to hypoxia dopaminergic neuron differentiation cellular response to transforming growth factor beta stimulus cellular response to BMP stimulus regulation of dopaminergic neuron differentiation midbrain morphogenesis
          It has to be done as per old AB suggested Products section.
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