Invitrogen

SFTPB Polyclonal Antibody

2 Published Figures

5 References

View all (17) SFTPB antibodies

Datasheet

Protocols

Questions & Answers

Datasheet

Protocols

Questions & Answers

Antibody Testing Data (2)
Published Figures (2)

SFTPB Antibody in Immunohistochemistry (Paraffin) (IHC (P))

FIGURE: 1 / 4

SFTPB Antibody (PA5-42000) in IHC (P)

Immunohistochemistry (paraffin-embedded) analysis of human lung tissue using an anti-Surfactant Protein B polyclonal antibody (Product # PA5-42000).; Secondary Antibody: Donkey anti-Rabbit-Cy3.

SFTPB Antibody in Immunohistochemistry (IHC)

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Product Details

PA5-42000

Applications

Tested Dilution

Publications

Western Blot (WB)

0.2-1 µg/mL

View 1 publication 1 publication

Immunohistochemistry (IHC)

View 2 publications 2 publications

Immunohistochemistry (Paraffin) (IHC (P))

1:200

View 2 publications 2 publications

Product Specifications

Species Reactivity

Human, Mouse, Rat

Published species

Human, Mouse, Rhesus monkey

Host/Isotype

Rabbit / IgG

Class

Polyclonal

Type

Antibody

Immunogen

Synthetic peptide directed towards the middle region of human SFTPB

Conjugate

Unconjugated Unconjugated Unconjugated

Form

Liquid

Concentration

0.5 mg/mL

Purification

Affinity Chromatography

Storage buffer

PBS with 2% sucrose

Contains

0.09% sodium azide

Storage conditions

-20°C, Avoid Freeze/Thaw Cycles

Shipping conditions

Ambient (domestic); Wet ice (international)

AB_2609628

Product Specific Information

Peptide sequence: PGALQARPGP HTQDLSEQQF PIPLPYCWLC RALIKRIQAM IPKGALAVAV

Sequence homology: Cow: 86%; Dog: 100%; Guinea Pig: 100%; Human: 100%; Mouse: 92%; Rabbit: 85%; Rat: 100%; Sheep: 79%

Target Information

Pulmonary surfactant is a complex mixture of phospholipids and proteins that is secreted from type II cells in alveoli and reduces the surface tension at the alveolar air-liquid interface, providing alveolar stability necessary for normal ventilation. Four distinct proteins isolated from pulmonary surfactant are termed surfactant proteins A, B, C, and D. SP-A (28-36kDa) and SP-D (43kDa) are collagenous carbohydrate-binding proteins, whereas SP-B (8-9kDa) and SP-C (4kDa) are non-collagenous hydrophobic proteins. SP-B is expressed in pulmonary adenocarcinomas with acinar, papillary, bronchioloalveolar, and solid growth patterns. Squamous cell and large cell carcinomas of the lung and nonpulmonary adenocarcinomas do not express SP-B. ProSP-B is glycosylated in the Golgi apparatus and undergoes carboxy- and amino-terminal proteolysis by a cathepsin D-like protease.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

Product References

Sigmar1 ablation leads to lung pathological changes associated with pulmonary fibrosis, inflammation, and altered surfactant proteins levels.

Frontiers in physiology

Remex NS,Abdullah CS,Aishwarya R,Nitu SS,Traylor J,Hartman B,King J,Bhuiyan MAN,Kevil CG,Orr AW,Bhuiyan MS

PA5-42000 was used in Western Blotting to determine the expression of Sigmar1 in the lungs.

Thu May 16 00:00:00 UTC 2024

Human distal lung maps and lineage hierarchies reveal a bipotent progenitor.

Nature

Kadur Lakshminarasimha Murthy P,Sontake V,Tata A,Kobayashi Y,Macadlo L,Okuda K,Conchola AS,Nakano S,Gregory S,Miller LA,Spence JR,Engelhardt JF,Boucher RC,Rock JR,Randell SH,Tata PR

PA5-42000 was used in Immunohistochemistry (Paraffin), Western Blot to revise human lung cell maps and lineage trajectories, and implicate an epithelial transitional state in primate lung regeneration and disease.

Fri Apr 01 00:00:00 UTC 2022

Defined conditions for long-term expansion of murine and human alveolar epithelial stem cells in three-dimensional cultures.

STAR protocols

Konishi S,Tata A,Tata PR

PA5-42000 was used in Immunohistochemistry to describe a detailed protocol for the isolation, purification, and culture of murine and human AT2s.

Fri Jun 17 00:00:00 UTC 2022

Human Lung Stem Cell-Based Alveolospheres Provide Insights into SARS-CoV-2-Mediated Interferon Responses and Pneumocyte Dysfunction.

Cell stem cell

Katsura H,Sontake V,Tata A,Kobayashi Y,Edwards CE,Heaton BE,Konkimalla A,Asakura T,Mikami Y,Fritch EJ,Lee PJ,Heaton NS,Boucher RC,Randell SH,Baric RS,Tata PR

PA5-42000 was used in Immunohistochemistry to report a feeder-free, scalable, chemically defined, and modular alveolosphere culture system for the propagation and differentiation of human alveolar type 2 cells/pneumocytes derived from primary lung tissue.

Thu Dec 03 00:00:00 UTC 2020

Human Lung Stem Cell-Based Alveolospheres Provide Insights into SARS-CoV-2-Mediated Interferon Responses and Pneumocyte Dysfunction.

Cell stem cell

Katsura H,Sontake V,Tata A,Kobayashi Y,Edwards CE,Heaton BE,Konkimalla A,Asakura T,Mikami Y,Fritch EJ,Lee PJ,Heaton NS,Boucher RC,Randell SH,Baric RS,Tata PR

Thu Dec 03 00:00:00 UTC 2020

Human Lung Stem Cell-Based Alveolospheres Provide Insights into SARS-CoV-2-Mediated Interferon Responses and Pneumocyte Dysfunction.

Cell stem cell

Katsura H,Sontake V,Tata A,Kobayashi Y,Edwards CE,Heaton BE,Konkimalla A,Asakura T,Mikami Y,Fritch EJ,Lee PJ,Heaton NS,Boucher RC,Randell SH,Baric RS,Tata PR

Thu Dec 03 00:00:00 UTC 2020

Bioinformatics

Protein Aliases: 18 kDa pulmonary-surfactant protein; 6 kDa protein; P SFTB3; Pulmonary surfactant-associated protein B; Pulmonary surfactant-associated proteolipid SPL(Phe); SFPB; SP-B; surfactant associated protein B; surfactant pulmonary-associated protein B; surfactant, pulmonary-associated protein B

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Gene Aliases: AI562151; PSP-B; SF-B; SFTB3; Sftp-3; SFTP3; SFTPB; SMDP1; SP-B

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UniProt ID: (Human) P07988, (Rat) P22355, (Mouse) P50405

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Entrez Gene ID: (Human) 6439, (Rat) 192155, (Mouse) 20388

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Function(s)

G-protein coupled receptor binding enzyme activator activity molecular_function

Process(es)

sphingolipid metabolic process respiratory gaseous exchange organ morphogenesis positive regulation of catalytic activity cellular protein metabolic process response to hypoxia circadian rhythm response to glucose response to lipopolysaccharide response to vitamin A regulation of liquid surface tension response to glucocorticoid response to hyperoxia response to interleukin-6 response to growth factor response to epidermal growth factor cellular response to mechanical stimulus cellular response to nitric oxide lipid metabolic process

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SFTPB Polyclonal Antibody

SFTPB Antibody (PA5-42000) in IHC (P)

Product Details

PA5-42000

Western Blot (WB)

Immunohistochemistry (IHC)

Immunohistochemistry (Paraffin) (IHC (P))

Species Reactivity

Published species

Host/Isotype

Class

Type

Immunogen

Conjugate

Form

Concentration

Purification

Storage buffer

Contains

Storage conditions

Shipping conditions

RRID

Product Specific Information

Target Information

Bioinformatics

Performance Guarantee

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