Invitrogen

SHANK1/SHANK3 Monoclonal Antibody (N367/51), PE

View all (5) SHANK1/SHANK3 antibodies

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Datasheet
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Datasheet
Protocols
Questions & Answers

Product Details

MA5-45676

Applications
Tested Dilution
Publications

Western Blot (WB)

1:1,000
-

Immunohistochemistry (IHC)

Assay-dependent
-

Immunocytochemistry (ICC/IF)

1:100
-
Product Specifications

Species Reactivity

Human, Mouse, Rat

Host/Isotype

Mouse / IgG2a

Class

Monoclonal

Type

Antibody

Clone

N367/51

Immunogen

Fusion protein amino acids 538-626 (SH3 domain) of rat SHANK3.
View immunogen

Conjugate

PE PE PE

Excitation/Emission Max

565/576 nm View spectra spectra

Form

Liquid

Concentration

1 mg/mL

Purification

Protein G

Storage buffer

95.64mM phosphate/2.48mM MES, pH 7.4, with 0.5M EDTA

Contains

no preservative

Storage conditions

4°C

Shipping conditions

Ambient (domestic); Wet ice (international)

RRID

AB_2932130

Product Specific Information

Mouse: 100% identity (89/89 amino acids identical). Human: 97% identity (87/89 amino acids identical). ~70% identity with SHANK1 and SHANK2.

1 µg/mL of MA5-45676 was sufficient for detection of SHANK1/SHANK3 in 20 µg of rat brain lysate by colorimetric immunoblot analysis using Goat anti-mouse IgG:HRP as the secondary antibody.|Detects approximately 190kDa. Cross-reacts with SHANK1. Does not cross-react with SHANK2.

This antibody was formerly sold as clone S367-51.

Target Information

Major scaffold postsynaptic density protein which interacts with multiple proteins and complexes to orchestrate the dendritic spine and synapse formation, maturation and maintenance. Interconnects receptors of the postsynaptic membrane including NMDA-type and metabotropic glutamate receptors via complexes with GKAP/PSD-95 and HOMER, respectively, and the actin-based cytoskeleton. Plays a role in the structural and functional organization of the dendritic spine and synaptic junction through the interaction with Arp2/3 and WAVE1 complex as well as the promotion of the F-actin clusters. By way of this control of actin dynamics, participates in the regulation of developing neurons growth cone motility and the NMDA receptor-signaling. Also modulates GRIA1 exocytosis and GRM5/MGLUR5 expression and signaling to control the AMPA and metabotropic glutamate receptor-mediated synaptic transmission and plasticity. May be required at an early stage of synapse formation and be inhibited by IGF1 to promote synapse maturation. {PubMed:21606927, PubMed:21795692, PubMed:23100419, PubMed:23897824, PubMed:24089484}.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

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Bioinformatics

Protein Aliases: GKAP/SAPAP interacting protein; GKAP/SAPAP-interacting protein; proline rich synapse associated protein 2; Proline-rich synapse-associated protein 2; ProSAP2; SAPAP-interacting protein synamon; SH3 and multiple ankyrin repeat domains 1; SH3 and multiple ankyrin repeat domains protein 1; SH3 and multiple ankyrin repeat domains protein 3; SH3 and multiple ankyrin repeat domains protein 3 variant c-3; SH3 and multiple ankyrin repeat domains protein 3 variant c-4; SH3 and multiple ankyrin repeat domains-like protein 3 variant 1; SH3 and multiple ankyrin repeat domains-like protein 3 variant 2; SH3 and multiple ankyrin repeat domains-like protein 3 variant 3; SH3 and multiple ankyrin repeat domains-like protein 3 variant c-1; SH3 and multiple ankyrin repeat domains-like protein 3 variant c-2; SH3 and multiple ankyrin repeat domains-like protein 3 variant d-1; SH3 and multiple ankyrin repeat domains-like protein 3 variant d-2; SH3 and multiple ankyrin repeat domains-like protein 3 variant d-3; SH3 and multiple ankyrin repeat domains-like protein 3 variant e-1; SH3 and multiple ankyrin repeat domains-like protein 3 variant e-2; SH3/ankyrin domain gene 1; SH3/ankyrin domain gene 3; shank postsynaptic density protein; Shank postsynaptic density protein 3a; Shank1; Shank3; shank3 splice variant II; shank3 splice variant III; shank3 splice variant IV; shank3e splice variant III; shank3e splice variant IV; Somatostatin receptor-interacting protein; SPANK-1; SPANK-2; SSTR-interacting protein; Synamon

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Gene Aliases: AI841104; DEL22q13.3; Kiaa1650; PROSAP2; PSAP2; SCZD15; SHANK1; Shank1a; Shank3; Shank3b; SHANK3c-3; SHANK3c-4; SPANK-1; SPANK-2; Spank1; SSTRIP; synamon

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UniProt ID: (Human) Q9Y566, (Rat) Q9WV48, (Mouse) D3YZU1, (Mouse) Q4ACU6, (Rat) Q9JLU4

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Entrez Gene ID: (Human) 50944, (Human) 85358, (Rat) 78957, (Mouse) 243961, (Mouse) 58234, (Rat) 59312

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Function(s)
protein binding protein C-terminus binding SH3 domain binding GKAP/Homer scaffold activity somatostatin receptor binding protein complex binding ionotropic glutamate receptor binding identical protein binding ankyrin repeat binding scaffold protein binding actin binding zinc ion binding receptor signaling complex scaffold activity protein self-association
Process(es)
protein complex assembly cytoskeletal anchoring at plasma membrane long-term memory associative learning adult behavior negative regulation of actin filament bundle assembly social behavior protein localization to synapse olfactory behavior habituation neuromuscular process controlling balance determination of affect righting reflex synapse maturation dendritic spine morphogenesis positive regulation of dendritic spine development vocalization behavior regulation of alpha-amino-3-hydroxy-5-methyl-4-isoxazole propionate selective glutamate receptor activity positive regulation of excitatory postsynaptic potential MAPK cascade embryonic epithelial tube formation synapse assembly learning memory striatal medium spiny neuron differentiation locomotory exploration behavior vocal learning negative regulation of cell volume positive regulation of long-term neuronal synaptic plasticity brain morphogenesis protein oligomerization positive regulation of synapse structural plasticity positive regulation of synaptic transmission, glutamatergic regulation of dendritic spine morphogenesis postsynaptic density assembly AMPA glutamate receptor clustering NMDA glutamate receptor clustering guanylate kinase-associated protein clustering regulation of long-term synaptic potentiation positive regulation of long-term synaptic potentiation positive regulation of glutamate receptor signaling pathway regulation of long term synaptic depression regulation of grooming behavior regulation of behavioral fear response positive regulation of alpha-amino-3-hydroxy-5-methyl-4-isoxazole propionate selective glutamate receptor activity nervous system development cell differentiation
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