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Invitrogen
SHC Polyclonal Antibody
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Product Details
PA5-79996
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Human,
Mouse,
Rat
Host/Isotype
Rabbit
/ IgG
Class
Polyclonal
Type
Antibody
Immunogen
A synthetic peptide corresponding to a sequence at the C-terminus of human SHC (536-564aa VDPEGVVRTKDHRFESVSHLISYHMDNHL).
Conjugate
Form
Lyophilized
Concentration
500 µg/mL
Purification
Antigen affinity chromatography
Storage buffer
PBS with 5mg BSA
Contains
0.05mg sodium azide
Storage conditions
-20°C
Shipping conditions
Ambient (domestic); Wet ice (international)
RRID
AB_2747111
Product Specific Information
Reconstitute with 0.2 mL of distilled water to yield a concentration of 500 µg/mL.
Positive Control - WB: human A431 whole cell, human Hela whole cell, human HepG2 whole cell, human Jurkat whole cell, human K562 whole cell, human THP-1 whole cell, rat C6 whole cell, mouse thymus tissue, mouse RAW2467 whole cell, mouse NIH3T3 whole cell. IHC: Mouse Brain tissue, Rat Brain tissue, Human Lung Cancer tissue IHC-F: Human Placenta tissue.
Target Information
Skp2 (S-phase Kinase-associated Protein 2) belongs to the family of F-box proteins that interact with the Cyclin A-Cdk2 complex. Skp2 is essential for the G1-S transition in both transformed cells and diploid fibroblasts. Biochemical and genetic experiments have demonstrated that Skp2 is required for the ubiquitination and consequent degradation of p27 in cultured mammalian cells and in vitro reconstitution assays. In normal tissues, Skp2 is expressed in tonsil and placenta. In tumor tissues, Skp2 is expressed widely, in some colon, prostate, pancreas, and skin cancers, with high expression in lung, breast, ovarian, and endometrial cancers. Research suggests that Skp2 plays a role in oncogenesis, particularly in the pathogenesis of lymphomas and breast cancers.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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Bioinformatics
Protein Aliases: FLJ26504; OTTHUMP00000035472; RP11-307C12.1; SH2 domain protein C1; SHC (Src homology 2 domain containing) transforming protein 1; SHC (Src homology 2 domain-containing) transforming protein 1; SHC-transforming protein 1; SHC-transforming protein 3; SHC-transforming protein A; src homology 2 domain-containing transforming protein C1; Src homology 2 domain-containing-transforming protein C1
Gene Aliases: p66; P66shc; SHC; SHC1; SHCA
UniProt ID: (Human) P29353, (Rat) Q5M824, (Mouse) P98083
Entrez Gene ID: (Human) 6464, (Rat) 85385, (Mouse) 20416
phosphotyrosine binding
transmembrane receptor protein tyrosine kinase adaptor activity
Ras guanyl-nucleotide exchange factor activity
epidermal growth factor receptor binding
insulin receptor binding
insulin-like growth factor receptor binding
neurotrophin TRKA receptor binding
protein binding
phospholipid binding
ephrin receptor binding
protein phosphatase 2A binding
protein kinase binding
receptor tyrosine kinase binding
protein complex binding
phosphoprotein binding
MAPK cascade
activation of MAPK activity
angiogenesis
response to hypoxia
epidermal growth factor receptor signaling pathway
heart development
aging
insulin receptor signaling pathway
response to toxic substance
response to organic cyclic compound
single organismal cell-cell adhesion
actin cytoskeleton organization
neuron differentiation
organ regeneration
neuron projection development
actin cytoskeleton reorganization
response to insulin
cellular response to insulin stimulus
response to nicotine
intracellular signal transduction
regulation of growth
regulation of cell proliferation
response to hydrogen peroxide
positive regulation of DNA replication
positive regulation of vasoconstriction
positive regulation of smooth muscle cell proliferation
response to glucocorticoid
regulation of epidermal growth factor-activated receptor activity
Ras protein signal transduction
positive regulation of cell proliferation
viral process
IRE1-mediated unfolded protein response
Fc-epsilon receptor signaling pathway
ERBB2 signaling pathway
positive regulation of GTPase activity
leukocyte migration
cellular response to growth factor stimulus
signal transduction
Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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