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          Clicking the images or links will redirect you to a website hosted by BenchSci that provides third-party scientific content. Neither the content nor the BenchSci technology and processes for selection have been evaluated by us; we are providing them as-is and without warranty of any kind, including for use or application of the Thermo Fisher Scientific products presented.

          • Primary Antibodies ›
          • SHH Antibodies

          Proteintech

          SHH Polyclonal Antibody

          70 References
          View all (26) SHH antibodies
          Datasheet
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          Datasheet
          Protocols
          Questions & Answers

          Cite SHH Polyclonal Antibody

          • Antibody Testing Data (20)
          SHH Antibody in Immunocytochemistry (ICC/IF)
          Group 53 Created with Sketch.
          SHH Antibody in Immunocytochemistry (ICC/IF)
          Group 53 Created with Sketch.

          FIGURE: 1 / 20

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          SHH Antibody (20697-1-AP) in ICC/IF

          Frozen tissue section of adult mouse kidney was stained for acetylated ɑ-tubulin (magenta, Cat. No CL488-66200), CD31/PECAM-1 (white), and Shh (green, Cat. No Product # 20697-1-AP) with DAPI as a counterstain for visualizing the nucleus (blue). acetylated ɑ-tubulin stains primary cilia and was conjugated to Coralite-488 fluorescent dye and psedocolored to magenta. CD31 stains endocardial/endothelial cells and was visualized with an Alexa Fluor 647 secondary antibody and psedocolored to white. Shh stains Sonic Hedgehog and was visualized wi... View More {{ $ctrl.currentElement.advancedVerification.fullName }} validation info. View more
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          SHH Antibody in Immunocytochemistry (ICC/IF)
          SHH Antibody in Immunocytochemistry (ICC/IF)
          SHH Antibody in Immunohistochemistry (Paraffin) (IHC (P))
          SHH Antibody in Immunohistochemistry (Paraffin) (IHC (P))
          SHH Antibody in Immunohistochemistry (Paraffin) (IHC (P))
          SHH Antibody in Immunohistochemistry (Paraffin) (IHC (P))
          SHH Antibody in Immunohistochemistry (Paraffin) (IHC (P))
          SHH Antibody in Immunohistochemistry (Paraffin) (IHC (P))
          SHH Antibody in Immunohistochemistry (Paraffin) (IHC (P))
          SHH Antibody in Immunohistochemistry (Paraffin) (IHC (P))
          SHH Antibody in Immunohistochemistry (Paraffin) (IHC (P))
          SHH Antibody in Immunohistochemistry (Paraffin) (IHC (P))
          SHH Antibody in Immunohistochemistry (Paraffin) (IHC (P))
          SHH Antibody in Immunohistochemistry (Paraffin) (IHC (P))
          SHH Antibody in Western Blot (WB)
          SHH Antibody in Western Blot (WB)
          SHH Antibody in Western Blot (WB)
          SHH Antibody in Western Blot (WB)
          SHH Antibody in Flow Cytometry (Flow)
          SHH Antibody in Immunoprecipitation (IP)

          Product Details

          20697-1-AP

          Applications
          Tested Dilution
          Publications

          Western Blot (WB)

          1:500-1:1,000
          View 53 publications 53 publications

          Immunohistochemistry (IHC)

          -
          View 9 publications 9 publications

          Immunohistochemistry (Paraffin) (IHC (P))

          1:20-1:200
          -

          Immunocytochemistry (ICC/IF)

          1:10-1:100
          View 6 publications 6 publications

          Flow Cytometry (Flow)

          0.2 µg/1x10^6 cells
          -

          Immunoprecipitation (IP)

          0.5-4.0 µg
          -

          Miscellaneous PubMed (MISC)

          -
          View 1 publication 1 publication

          Not applicable (N/A)

          -
          View 1 publication 1 publication
          Product Specifications

          Species Reactivity

          Human, Mouse, Rat

          Published species

          Human, Mink, Mouse, Rat

          Host/Isotype

          Rabbit / IgG

          Class

          Polyclonal

          Type

          Antibody

          Immunogen

          Peptide

          Conjugate

          Unconjugated Unconjugated Unconjugated

          Form

          Liquid

          Concentration

          0.4 mg/mL

          Purification

          Antigen affinity chromatography

          Storage buffer

          PBS, pH 7.3, with 50% glycerol

          Contains

          0.02% sodium azide

          Storage conditions

          -20°C

          Shipping conditions

          Wet ice (domestic); Dry ice (international)

          Target Information

          Sonic Hedgehog (SHH), which is expressed only during embryogenesis, is instrumental in patterning the early embryo. It has been implicated as the key inductive signal in patterning of the ventral neural tube, the anterior-posterior limb axis, and the ventral somites. Of three human proteins showing sequence and functional similarity to the Sonic Hedgehog protein of Drosophila, this protein is the most similar. The protein is made as a precursor that is autocatalytically cleaved, the N-terminal portion is soluble and contains the signalling activity while the C-terminal portion is involved in precursor processing. More importantly, the C-terminal product covalently attaches a cholesterol moiety to the N-terminal product, restricting the N-terminal product to the cell surface and preventing it from freely diffusing throughout the developing embryo. Defects in this protein or in its signalling pathway are a cause of holoprosencephaly (HPE), a disorder in which the developing forebrain fails to correctly separate into right and left hemispheres. HPE is manifested by facial deformities. In addition, it is thought that mutations in this gene or in its signalling pathway may be responsible for VACTERL syndrome, which is characterized by vertebral defects, anal atresia, tracheoesophageal fistula with esophageal atresia, radial and renal dysplasia, cardiac anomalies, and limb abnormalities.

          For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

          Bioinformatics

          Protein Aliases: hemimelic extra toes; HHG-1; SHH; Shh unprocessed N-terminal signaling and C-terminal autoprocessing domains; ShhNC; short digits; sonic hedgehog homolog; Sonic hedgehog protein

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          Gene Aliases: 9530036O11Rik; Dsh; HHG1; HLP3; HPE3; Hx; Hxl3; M100081; MCOPCB5; SHH; SMMCI; TPT; TPTPS; Vhh-1

          View more View less

          UniProt ID: (Human) Q15465, (Mouse) Q62226, (Rat) Q63673

          View more View less

          Entrez Gene ID: (Human) 6469, (Mouse) 20423, (Rat) 29499

          View more View less

          Function(s)
          glycoprotein binding patched binding calcium ion binding protein binding glycosaminoglycan binding peptidase activity zinc ion binding morphogen activity laminin-1 binding hydrolase activity metal ion binding receptor binding
          Process(es)
          negative regulation of transcription from RNA polymerase II promoter angiogenesis patterning of blood vessels vasculogenesis metanephros development branching involved in ureteric bud morphogenesis cell fate specification neural crest cell migration kidney development neural tube formation hair follicle development vasculature development heart looping positive regulation of neuroblast proliferation positive regulation of mesenchymal cell proliferation osteoblast development lymphoid progenitor cell differentiation determination of left/right asymmetry in lateral mesoderm regulation of transcription, DNA-templated proteolysis endocytosis apoptotic process cell communication signal transduction smoothened signaling pathway positive regulation of hh target transcription factor activity cell-cell signaling multicellular organism development determination of left/right symmetry pattern specification process ectoderm development neuroblast proliferation axon guidance central nervous system development hindgut morphogenesis digestive tract mesoderm development heart development blood coagulation androgen metabolic process cell proliferation positive regulation of cell proliferation embryo development polarity specification of anterior/posterior axis anterior/posterior pattern specification dorsal/ventral pattern formation mesenchymal cell proliferation regulation of gene expression positive regulation of gene expression negative regulation of gene expression oligodendrocyte development striated muscle tissue development positive regulation of skeletal muscle cell proliferation myotube differentiation intein-mediated protein splicing spinal cord dorsal/ventral patterning ventral spinal cord interneuron specification spinal cord motor neuron differentiation thalamus development dorsal/ventral neural tube patterning cerebellar granule cell precursor proliferation smoothened signaling pathway involved in regulation of cerebellar granule cell precursor cell proliferation positive regulation of cerebellar granule cell precursor proliferation telencephalon regionalization establishment of cell polarity regulation of proteolysis positive regulation of Wnt signaling pathway negative regulation of Wnt signaling pathway respiratory tube development lung development embryonic limb morphogenesis negative regulation of cell migration male genitalia development prostate gland development thyroid gland development forebrain development midbrain development hindbrain development pancreas development hair follicle morphogenesis negative regulation of proteasomal ubiquitin-dependent protein catabolic process T cell differentiation in thymus positive regulation of T cell differentiation in thymus positive regulation of immature T cell proliferation in thymus negative regulation of transcription elongation from RNA polymerase II promoter protein localization to nucleus embryonic forelimb morphogenesis embryonic hindlimb morphogenesis regulation of cell proliferation negative regulation of T cell proliferation negative regulation of protein catabolic process positive regulation of protein import into nucleus odontogenesis of dentin-containing tooth odontogenesis regulation of odontogenesis embryonic digit morphogenesis camera-type eye development negative regulation of apoptotic process CD4-positive or CD8-positive, alpha-beta T cell lineage commitment tongue development tongue morphogenesis skin development positive thymic T cell selection negative thymic T cell selection intermediate filament organization cell fate commitment myoblast differentiation response to ethanol negative regulation of cell differentiation positive regulation of cell differentiation positive regulation of neuron differentiation positive regulation of smoothened signaling pathway positive regulation of transcription, DNA-templated positive regulation of transcription from RNA polymerase II promoter positive regulation of photoreceptor cell differentiation positive regulation of alpha-beta T cell differentiation negative regulation of alpha-beta T cell differentiation cell development thymus development digestive tract morphogenesis embryonic digestive tract morphogenesis embryonic organ development developmental growth embryonic morphogenesis embryonic foregut morphogenesis positive regulation of skeletal muscle tissue development organ formation anatomical structure formation involved in morphogenesis neuron fate commitment response to axon injury embryonic skeletal system development oligodendrocyte differentiation positive regulation of oligodendrocyte differentiation branching morphogenesis of an epithelial tube male genitalia morphogenesis inner ear development anatomical structure development formation of anatomical boundary stem cell development striated muscle cell differentiation positive regulation of striated muscle cell differentiation positive regulation of cell division Bergmann glial cell differentiation palate development canonical Wnt signaling pathway limb development limb bud formation positive regulation of penile erection lung morphogenesis lung epithelium development trachea development trachea morphogenesis epithelial tube branching involved in lung morphogenesis branching involved in prostate gland morphogenesis branching involved in salivary gland morphogenesis bud outgrowth involved in lung branching right lung development left lung development lung lobe morphogenesis lung-associated mesenchyme development prostatic bud formation primary prostatic bud elongation prostate epithelial cord elongation salivary gland cavitation epithelial cell proliferation involved in salivary gland morphogenesis epithelial-mesenchymal cell signaling regulation of prostatic bud formation epithelial-mesenchymal signaling involved in prostate gland development regulation of epithelial cell proliferation involved in prostate gland development positive regulation of epithelial cell proliferation involved in prostate gland development regulation of mesenchymal cell proliferation involved in prostate gland development mesenchymal smoothened signaling pathway involved in prostate gland development artery development mesenchymal cell proliferation involved in lung development vasculogenesis involved in coronary vascular morphogenesis somite development positive regulation of sclerotome development fungiform papilla development fungiform papilla morphogenesis fungiform papilla formation positive regulation of oligodendrocyte progenitor proliferation cellular response to lithium ion metanephric mesenchymal cell proliferation involved in metanephros development multicellular structure septum development negative regulation of canonical Wnt signaling pathway negative regulation of cholesterol efflux apoptotic signaling pathway regulation of protein localization to nucleus negative regulation of ureter smooth muscle cell differentiation positive regulation of ureter smooth muscle cell differentiation negative regulation of kidney smooth muscle cell differentiation positive regulation of kidney smooth muscle cell differentiation positive regulation of mesenchymal cell proliferation involved in ureter development negative regulation of mesenchymal cell apoptotic process ventral midline development embryonic pattern specification dopaminergic neuron differentiation renal system development regulation of nodal signaling pathway involved in determination of lateral mesoderm left/right asymmetry negative regulation of dopaminergic neuron differentiation response to hypoxia response to organonitrogen compound regulation of epithelial cell differentiation response to estradiol response to retinoic acid
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