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          Clicking the images or links will redirect you to a website hosted by BenchSci that provides third-party scientific content. Neither the content nor the BenchSci technology and processes for selection have been evaluated by us; we are providing them as-is and without warranty of any kind, including for use or application of the Thermo Fisher Scientific products presented.

          • Primary Antibodies ›
          • SHH Antibodies

          Invitrogen

          SHH Recombinant Rabbit Monoclonal Antibody (SC05-28)

          View all (26) SHH antibodies

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          Datasheet
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          Cite SHH Recombinant Rabbit Monoclonal Antibody (SC05-28)

          • Antibody Testing Data (9)
          SHH Antibody in Immunocytochemistry (ICC/IF)
          Group 53 Created with Sketch.
          SHH Antibody in Immunocytochemistry (ICC/IF)
          Group 53 Created with Sketch.

          FIGURE: 1 / 9

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          SHH Antibody (MA5-41159) in ICC/IF

          Immunocytochemistry/Immunofluorescence analysis of SHH in A549 cells (green) using SHH Recombinant Monoclonal Antibody (Product # MA5-41159). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS. The nuclear counter stain is DAPI (blue). {{ $ctrl.currentElement.advancedVerification.fullName }} validation info. View more
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          SHH Antibody in Immunocytochemistry (ICC/IF)
          SHH Antibody in Immunocytochemistry (ICC/IF)
          SHH Antibody in Immunocytochemistry (ICC/IF)
          SHH Antibody in Immunohistochemistry (Paraffin) (IHC (P))
          SHH Antibody in Immunohistochemistry (Paraffin) (IHC (P))
          SHH Antibody in Immunohistochemistry (Paraffin) (IHC (P))
          SHH Antibody in Immunohistochemistry (Paraffin) (IHC (P))
          SHH Antibody in Western Blot (WB)
          SHH Antibody in Flow Cytometry (Flow)

          Product Details

          MA5-41159

          Applications
          Tested Dilution
          Publications

          Western Blot (WB)

          1:1,000-1:2,000
          -

          Immunohistochemistry (Paraffin) (IHC (P))

          1:200-1:500
          -

          Immunocytochemistry (ICC/IF)

          1:50-1:200
          -

          Flow Cytometry (Flow)

          1:50-1:100
          -
          Product Specifications

          Species Reactivity

          Human

          Host/Isotype

          Rabbit / IgG

          Expression System

          HEK293 cells

          Class

          Recombinant Monoclonal

          Type

          Antibody

          Clone

          SC05-28

          Immunogen

          recombinant protein

          Conjugate

          Unconjugated Unconjugated Unconjugated

          Form

          Liquid

          Concentration

          1 mg/mL

          Purification

          Protein A

          Storage buffer

          TBS, pH 7.4, with 40% glycerol, 0.05% BSA

          Contains

          0.05% sodium azide

          Storage conditions

          Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.

          Shipping conditions

          Ambient (domestic); Wet ice (international)

          RRID

          AB_2898913

          Target Information

          Sonic Hedgehog (SHH), which is expressed only during embryogenesis, is instrumental in patterning the early embryo. It has been implicated as the key inductive signal in patterning of the ventral neural tube, the anterior-posterior limb axis, and the ventral somites. Of three human proteins showing sequence and functional similarity to the Sonic Hedgehog protein of Drosophila, this protein is the most similar. The protein is made as a precursor that is autocatalytically cleaved, the N-terminal portion is soluble and contains the signalling activity while the C-terminal portion is involved in precursor processing. More importantly, the C-terminal product covalently attaches a cholesterol moiety to the N-terminal product, restricting the N-terminal product to the cell surface and preventing it from freely diffusing throughout the developing embryo. Defects in this protein or in its signalling pathway are a cause of holoprosencephaly (HPE), a disorder in which the developing forebrain fails to correctly separate into right and left hemispheres. HPE is manifested by facial deformities. In addition, it is thought that mutations in this gene or in its signalling pathway may be responsible for VACTERL syndrome, which is characterized by vertebral defects, anal atresia, tracheoesophageal fistula with esophageal atresia, radial and renal dysplasia, cardiac anomalies, and limb abnormalities.

          For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

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          Cite this product

          Bioinformatics

          Protein Aliases: HHG-1; SHH; Shh unprocessed N-terminal signaling and C-terminal autoprocessing domains; ShhNC; sonic hedgehog homolog; Sonic hedgehog protein

          View more View less

          Gene Aliases: HHG1; HLP3; HPE3; MCOPCB5; SHH; SMMCI; TPT; TPTPS

          View more View less

          UniProt ID: (Human) Q15465

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          Entrez Gene ID: (Human) 6469

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          Function(s)
          glycoprotein binding patched binding calcium ion binding protein binding glycosaminoglycan binding peptidase activity zinc ion binding morphogen activity laminin-1 binding
          Process(es)
          negative regulation of transcription from RNA polymerase II promoter patterning of blood vessels vasculogenesis metanephros development branching involved in ureteric bud morphogenesis cell fate specification neural crest cell migration heart looping positive regulation of neuroblast proliferation osteoblast development lymphoid progenitor cell differentiation determination of left/right asymmetry in lateral mesoderm endocytosis smoothened signaling pathway positive regulation of hh target transcription factor activity cell-cell signaling pattern specification process ectoderm development neuroblast proliferation axon guidance central nervous system development ventral midline development hindgut morphogenesis heart development blood coagulation androgen metabolic process positive regulation of cell proliferation embryo development embryonic pattern specification polarity specification of anterior/posterior axis dorsal/ventral pattern formation oligodendrocyte development striated muscle tissue development positive regulation of skeletal muscle cell proliferation myotube differentiation intein-mediated protein splicing spinal cord dorsal/ventral patterning spinal cord motor neuron differentiation thalamus development dorsal/ventral neural tube patterning cerebellar granule cell precursor proliferation smoothened signaling pathway involved in regulation of cerebellar granule cell precursor cell proliferation telencephalon regionalization establishment of cell polarity regulation of proteolysis positive regulation of Wnt signaling pathway lung development embryonic limb morphogenesis negative regulation of cell migration male genitalia development prostate gland development thyroid gland development forebrain development midbrain development hindbrain development pancreas development hair follicle morphogenesis negative regulation of proteasomal ubiquitin-dependent protein catabolic process T cell differentiation in thymus positive regulation of T cell differentiation in thymus positive regulation of immature T cell proliferation in thymus negative regulation of transcription elongation from RNA polymerase II promoter protein localization to nucleus embryonic forelimb morphogenesis embryonic hindlimb morphogenesis regulation of cell proliferation negative regulation of T cell proliferation positive regulation of protein import into nucleus odontogenesis of dentin-containing tooth regulation of odontogenesis embryonic digit morphogenesis camera-type eye development negative regulation of apoptotic process CD4-positive or CD8-positive, alpha-beta T cell lineage commitment positive thymic T cell selection negative thymic T cell selection intermediate filament organization myoblast differentiation negative regulation of cell differentiation positive regulation of smoothened signaling pathway positive regulation of transcription, DNA-templated positive regulation of transcription from RNA polymerase II promoter positive regulation of alpha-beta T cell differentiation negative regulation of alpha-beta T cell differentiation cell development thymus development embryonic digestive tract morphogenesis embryonic foregut morphogenesis positive regulation of skeletal muscle tissue development organ formation neuron fate commitment embryonic skeletal system development positive regulation of oligodendrocyte differentiation branching morphogenesis of an epithelial tube inner ear development formation of anatomical boundary stem cell development positive regulation of striated muscle cell differentiation positive regulation of cell division Bergmann glial cell differentiation palate development canonical Wnt signaling pathway limb bud formation lung epithelium development trachea morphogenesis branching involved in salivary gland morphogenesis bud outgrowth involved in lung branching right lung development left lung development lung lobe morphogenesis lung-associated mesenchyme development primary prostatic bud elongation prostate epithelial cord elongation salivary gland cavitation epithelial cell proliferation involved in salivary gland morphogenesis regulation of prostatic bud formation epithelial-mesenchymal signaling involved in prostate gland development positive regulation of epithelial cell proliferation involved in prostate gland development regulation of mesenchymal cell proliferation involved in prostate gland development mesenchymal smoothened signaling pathway involved in prostate gland development artery development mesenchymal cell proliferation involved in lung development somite development positive regulation of sclerotome development cellular response to lithium ion dopaminergic neuron differentiation renal system development metanephric mesenchymal cell proliferation involved in metanephros development multicellular structure septum development negative regulation of canonical Wnt signaling pathway negative regulation of cholesterol efflux apoptotic signaling pathway regulation of nodal signaling pathway involved in determination of lateral mesoderm left/right asymmetry regulation of protein localization to nucleus negative regulation of dopaminergic neuron differentiation negative regulation of ureter smooth muscle cell differentiation positive regulation of ureter smooth muscle cell differentiation negative regulation of kidney smooth muscle cell differentiation positive regulation of kidney smooth muscle cell differentiation positive regulation of mesenchymal cell proliferation involved in ureter development negative regulation of mesenchymal cell apoptotic process
          It has to be done as per old AB suggested Products section.
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