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SHP2 Antibody (710867) in WB
Western blot analysis was performed on whole cell extracts (30 µg lysate) of HeLa (Lane 1), Jurkat (Lane 2), K562 (Lane 3), U-87 MG (Lane 4), MCF7 (Lane 5), PC-3 (Lane 6). The blots were probed with Anti-SHP2 Recombinant Rabbit Superclonal™ Antibody (Product # 710867, 1-2 µg/mL) and detected by chemiluminescence using Goat anti-Rabbit IgG (Heavy Chain) Superclonal™ Secondary Antibody, HRP conjugate (Product # A27036, 0.4 µg/mL, 1:2500 dilution). A 65 kDa band corresponding to SHP2 was observed. Known quantity of protein samples were electr... View More
Product Details
710867
Product Specifications
Species Reactivity
Human
Host/Isotype
Rabbit
/ IgG
Expression System
Expi293
Class
Recombinant Superclonal
Type
Antibody
Clone
16HCLC
Immunogen
Protein corresponding to Human PTPN11 (aa 2-597)
Conjugate
Form
Liquid
Concentration
0.5 mg/mL
Purification
Protein A
Storage buffer
PBS, pH 7.2
Contains
0.09% sodium azide
Storage conditions
Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.
Shipping conditions
Wet ice
RRID
AB_2608102
Product Specific Information
This antibody is predicted to react with Monkey, Mouse and Rat.
Recombinant rabbit Superclonal™ antibodies are unique offerings from Thermo Fisher Scientific. They are comprised of a selection of multiple different recombinant monoclonal antibodies, providing the best of both worlds - the sensitivity of polyclonal antibodies with the specificity of monoclonal antibodies - all delivered with the consistency only found in a recombinant antibody. While functionally the same as a polyclonal antibody - recognizing multiple epitope sites on the target and producing higher detection sensitivity for low abundance targets - a recombinant rabbit Superclonal™ antibody has a known mixture of light and heavy chains. The exact population can be produced in every lot, circumventing the biological variability typically associated with polyclonal antibody production. Note: Formerly called “Recombinant polyclonal antibody”, this product is now rebranded as “Recombinant Superclonal™ antibody”. The physical product and the performance remain unchanged.
Target Information
The protein encoded by this gene is a member of the protein tyrosine phosphatase (PTP) family. PTPs are known to be signaling molecules that regulate a variety of cellular processes including cell growth, differentiation, mitotic cycle, and oncogenic transformation. This PTP contains two tandem Src homology-2 domains, which function as phospho-tyrosine binding domains and mediate the interaction of this PTP with its substrates. This PTP is widely expressed in most tissues and plays a regulatory role in various cell signaling events that are important for a diversity of cell functions, such as mitogenic activation, metabolic control, transcription regulation, and cell migration. Mutations in this gene are a cause of Noonan syndrome as well as acute myeloid leukemia.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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Bioinformatics
Protein Aliases: HCP; HGNC:9644; MGC14433; Noonan syndrome 1; OTTHUMP00000166108; Protein-tyrosine phosphatase 1D; Protein-tyrosine phosphatase 2C; PTP-1D; PTP-2C; PTP1C; SH-PTP2; SH-PTP3; SHP-2; Tyrosine-protein phosphatase non-receptor type 11
Gene Aliases: BPTP3; CFC; JMML; METCDS; NS1; PTP-1D; PTP2C; PTPN11; SH-PTP2; SH-PTP3; SHP2; SHPTP2
UniProt ID: (Human) Q06124
Entrez Gene ID: (Human) 5781
phosphoprotein phosphatase activity
protein tyrosine phosphatase activity
non-membrane spanning protein tyrosine phosphatase activity
SH3/SH2 adaptor activity
insulin receptor binding
protein binding
1-phosphatidylinositol-3-kinase activity
protein domain specific binding
receptor tyrosine kinase binding
D1 dopamine receptor binding
phospholipase binding
insulin receptor substrate binding
phosphatidylinositol-4,5-bisphosphate 3-kinase activity
peptide hormone receptor binding
DNA damage checkpoint
activation of MAPK activity
triglyceride metabolic process
epidermal growth factor receptor signaling pathway
integrin-mediated signaling pathway
axonogenesis
brain development
heart development
fibroblast growth factor receptor signaling pathway
hormone-mediated signaling pathway
regulation of phosphatidylinositol 3-kinase signaling
cerebellar cortex formation
platelet activation
platelet formation
T cell costimulation
microvillus organization
abortive mitotic cell cycle
regulation of cell adhesion mediated by integrin
negative regulation of cell adhesion mediated by integrin
multicellular organism growth
organ growth
peptidyl-tyrosine dephosphorylation
megakaryocyte development
phosphatidylinositol-3-phosphate biosynthetic process
atrioventricular canal development
ERBB signaling pathway
regulation of multicellular organism growth
hormone metabolic process
glucose homeostasis
regulation of protein complex assembly
positive regulation of mitotic cell cycle
negative regulation of insulin secretion
regulation of protein export from nucleus
phosphatidylinositol phosphorylation
positive regulation of hormone secretion
platelet-derived growth factor receptor signaling pathway
neurotrophin TRK receptor signaling pathway
ephrin receptor signaling pathway
phosphatidylinositol-mediated signaling
multicellular organismal reproductive process
genitalia development
inner ear development
homeostasis of number of cells within a tissue
leukocyte migration
negative regulation of cortisol secretion
Bergmann glial cell differentiation
negative regulation of growth hormone secretion
face morphogenesis
regulation of type I interferon-mediated signaling pathway
intestinal epithelial cell migration
positive regulation of ERK1 and ERK2 cascade
positive regulation of glucose import in response to insulin stimulus
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If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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