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FIGURE: 1 / 1
SIAH1/2 Antibody (BS-3634R) in WB
Lane 1: mouse spleen lysates Lane 2: mouse brain lysates probed with Anti SIAH2 Polyclonal Antibody, Unconjugated (bs-3634R) at 1:200 in 4°C. Followed by conjugation to secondary antibody (bs-0295G-HRP) at 1:3000 90 min in 37°C. Predicted band 36 kD. Observed band size: 36 kD.
Product Details
BS-3634R
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Human,
Mouse,
Rat
Host/Isotype
Rabbit
/ IgG
Class
Polyclonal
Type
Antibody
Immunogen
KLH conjugated synthetic peptide derived from human SIAH2.
Conjugate
Form
Liquid
Concentration
1 mg/mL
Purification
Protein A
Storage buffer
0.01M TBS, pH 7.4, with 50% glycerol, 1% BSA
Contains
0.02% ProClin 300
Storage conditions
-20°C
Shipping conditions
Ambient (domestic); Wet ice (international)
Target Information
SIAH, the human homologue of the Drosophila seven in absentia (sina) gene, is a tumor suppressor protein that is expressed in intestinal epithelium and activated during apoptosis. Human SIAH proteins are produced as two distinct gene products, SIAH-1, and a slightly larger protein SIAH-2, which share a highly conserved C-terminal sequence and differ in their N-terminal regions. SIAH-1 is a 32 kDa protein that contains an N-terminal RING-finger domain, which is required for proteolysis, and a cystein-rich C-terminal domain, which regulates oligomerization and SIAH binding to target proteins. As a tumor suppressor, SIAH-1 binds DCC (deleted in colorectal cancer) and regulates DCC degradation via the ubiquitin-proteasome pathway. SIAH-1 also binds a Bcl-2 related protein, Bag-1, thereby inhibiting cell growth. The majority of SIAH-1 is localized to the nucleus, however a small percentage is detected in the cytoplasm. This nuclear localization suggests that SIAH proteins may interact with other nuclear matrix proteins and DNA.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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Bioinformatics
Protein Aliases: E3 ubiquitin-protein ligase SIAH1; E3 ubiquitin-protein ligase SIAH2; EC 6.3.2; FLJ08065; hSIAH1; mSiah2; RING-type E3 ubiquitin transferase SIAH1; RING-type E3 ubiquitin transferase SIAH2; seven in absentia 1A; seven in absentia 2; Seven in absentia homolog 1; Seven in absentia homolog 2; Siah-1; Siah-1a; Siah-2
Gene Aliases: AA415433; hSiah2; HUMSIAH; SIAH; SIAH1; SIAH1A; SIAH2; Sinh2
UniProt ID: (Human) Q8IUQ4, (Human) O43255, (Rat) Q920M9, (Mouse) Q06986, (Rat) Q8R4T2
Entrez Gene ID: (Human) 6477, (Human) 6478, (Rat) 140941, (Mouse) 20439, (Rat) 140593
ubiquitin-protein transferase activity
protein binding
protein C-terminus binding
zinc ion binding
ligase activity
identical protein binding
ubiquitin protein ligase activity
transcription corepressor activity
ubiquitin conjugating enzyme binding
protein homodimerization activity
UDP-N-acetylmuramoylalanyl-D-glutamyl-2,6-diaminopimelate-D-alanyl-D-alanine ligase activity
ribosomal S6-glutamic acid ligase activity
coenzyme F420-0 gamma-glutamyl ligase activity
coenzyme F420-2 alpha-glutamyl ligase activity
metal ion binding
protein-glycine ligase activity
protein-glycine ligase activity, initiating
protein-glycine ligase activity, elongating
tubulin-glycine ligase activity
protein-glutamic acid ligase activity
tubulin-glutamic acid ligase activity
protein polyubiquitination
ubiquitin-dependent protein catabolic process
apoptotic process
cell cycle
small GTPase mediated signal transduction
axon guidance
regulation of protein ubiquitination
protein ubiquitination involved in ubiquitin-dependent protein catabolic process
negative regulation of apoptotic process
negative regulation of cysteine-type endopeptidase activity involved in apoptotic process
proteasome-mediated ubiquitin-dependent protein catabolic process
cellular protein catabolic process
cellular protein metabolic process
negative regulation of canonical Wnt signaling pathway
negative regulation of nucleic acid-templated transcription
negative regulation of extrinsic apoptotic signaling pathway
spermatogenesis
nervous system development
anatomical structure morphogenesis
protein catabolic process
protein destabilization
positive regulation of apoptotic process
neuron apoptotic process
positive regulation of intrinsic apoptotic signaling pathway
multicellular organism development
protein ubiquitination
male meiosis I
post-embryonic development
cell differentiation
regulation of multicellular organism growth
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