Proteintech

SIAH1 Polyclonal Antibody

SIAH1 Antibody in Immunohistochemistry (Paraffin) (IHC (P))

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SIAH1 Antibody (13886-1-AP) in IHC (P)

Immunohistochemistry of paraffin-embedded mouse testis tissue slide using 13886-1-AP ( SIAH1 antibody) at dilution of 1:100 (under 40x lens) heat mediated antigen retrieved with Tris-EDTA buffer (pH 9).

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Product Details

13886-1-AP

Applications

Tested Dilution

Publications

Western Blot (WB)

1:500-1:1,000

View 3 publications 3 publications

Immunohistochemistry (Paraffin) (IHC (P))

1:50-1:500

Product Specifications

Species Reactivity

Human, Mouse

Host/Isotype

Rabbit / IgG

Class

Polyclonal

Type

Antibody

Immunogen

SIAH1 Fusion Protein Ag4336 (1-282 aa encoded by BC035562)

Conjugate

Unconjugated Unconjugated Unconjugated

Form

Liquid

Concentration

0.2 mg/mL

Purification

Antigen affinity chromatography

Storage buffer

PBS, pH 7.3, with 50% glycerol

Contains

0.02% sodium azide

Storage conditions

-20°C

Shipping conditions

Wet ice (domestic); Dry ice (international)

Product Specific Information

Immunogen sequence: MSRQTATAL PTGTSKCPPS QRVPALTGTT ASNNDLASLF ECPVCFDYVL PPILQCQSGH LVCSNCRPKL TCCPTCRGPL GSIRNLAMEK VANSVLFPCK YASSGCEITL PHTEKADHEE LCEFRPYSCP CPGASCKWQG SLDAVMPHLM HQHKSITTLQ GEDIVFLATD INLPGAVDWV MMQSCFGFHF MLVLEKQEKY DGHQQFFAIV QLIGTRKQAE NFAYRLELNG HRRRLTWEAT PRSIHEGIAT AIMNSDCLVF DTSIAQLFAE NGNLGINVTI SMC (1-282 aa encoded by BC035562)

Target Information

This gene encodes a protein that is a member of the seven in absentia homolog family. The protein is an E3 ligase and is involved in ubiquitination and proteasome-mediated degradation of specific proteins. The activity of this ubiquitin ligase has been implicated in the development of certain forms of Parkinson's disease, the regulation of the cellular response to hypoxia and induction of apoptosis. Alternative splicing results in several additional transcript variants, some encoding different isoforms and others that have not been fully characterized.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

Product References

Cathepsin K promotes the proliferation of hepatocellular carcinoma cells through induction of SIAH1 ubiquitination and degradation.

iScience

Zhang C,Liu Z,Wang X,Zhang B,Cui L,Hu Q,Hu B,Cao K,Shan W,Shi H,Wang R

Fri Jun 16 00:00:00 UTC 2023

Siah1 promotes the proliferation of NSCLC cells through ubiquitinating and stabilizing Notch1.

Experimental cell research

Liu Y,Li Q,Geng L,Zhou X,Zhou X,Dong Y,Zhang Y

Sat Oct 01 00:00:00 UTC 2022

Regulation of EZH2 by SMYD2-Mediated Lysine Methylation Is Implicated in Tumorigenesis.

Cell reports

Zeng Y,Qiu R,Yang Y,Gao T,Zheng Y,Huang W,Gao J,Zhang K,Liu R,Wang S,Hou Y,Yu W,Leng S,Feng D,Liu W,Zhang X,Wang Y

Tue Nov 05 00:00:00 UTC 2019

Bioinformatics

Protein Aliases: E3 ubiquitin-protein ligase SIAH1; E3 ubiquitin-protein ligase SIAH1A; E3 ubiquitin-protein ligase SIAH1B; FLJ08065; h HUMSIAH; hSIAH1; mSiah-1a; RING-type E3 ubiquitin transferase SIAH1; RING-type E3 ubiquitin transferase SIAH1A; RING-type E3 ubiquitin transferase SIAH1B; Seven in absentia homolog 1; Seven in absentia homolog 1a; Seven in absentia homolog 1b; Siah-1; Siah-1a; Siah-1b

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Gene Aliases: AA960570; AA982064; AI853500; HUMSIAH; siah-1b; SIAH1; SIAH1A; Siah1b; Sinh1a; Sinh1b

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UniProt ID: (Human) Q8IUQ4, (Mouse) P61092, (Mouse) Q06985

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Entrez Gene ID: (Human) 6477, (Mouse) 20437, (Mouse) 20438

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Function(s)

ubiquitin-protein transferase activity protein binding protein C-terminus binding zinc ion binding ligase activity identical protein binding ubiquitin protein ligase activity UDP-N-acetylmuramoylalanyl-D-glutamyl-2,6-diaminopimelate-D-alanyl-D-alanine ligase activity ribosomal S6-glutamic acid ligase activity protein homodimerization activity coenzyme F420-0 gamma-glutamyl ligase activity coenzyme F420-2 alpha-glutamyl ligase activity metal ion binding protein-glycine ligase activity protein-glycine ligase activity, initiating protein-glycine ligase activity, elongating tubulin-glycine ligase activity protein-glutamic acid ligase activity tubulin-glutamic acid ligase activity

Process(es)

protein polyubiquitination ubiquitin-dependent protein catabolic process apoptotic process cell cycle spermatogenesis nervous system development axon guidance anatomical structure morphogenesis protein catabolic process protein destabilization protein ubiquitination involved in ubiquitin-dependent protein catabolic process positive regulation of apoptotic process proteasome-mediated ubiquitin-dependent protein catabolic process cellular protein metabolic process neuron apoptotic process positive regulation of intrinsic apoptotic signaling pathway male meiosis I multicellular organism development post-embryonic development protein ubiquitination cell differentiation regulation of multicellular organism growth

Performance Guarantee

If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*

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SIAH1 Polyclonal Antibody

SIAH1 Antibody (13886-1-AP) in IHC (P)

Product Details

13886-1-AP

Western Blot (WB)

Immunohistochemistry (Paraffin) (IHC (P))

Species Reactivity

Host/Isotype

Class

Type

Immunogen

Conjugate

Form

Concentration

Purification

Storage buffer

Contains

Storage conditions

Shipping conditions

Product Specific Information

Target Information

Bioinformatics

Performance Guarantee

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