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Invitrogen
SIAH1/SIAH2 Monoclonal Antibody (8G7H12)
FIGURE: 1 / 2
SIAH1/SIAH2 Antibody (MA5-24781) in WB
Western blot analysis of SIAH1/SIAH2 in drosophila SINA and human SIAH. Samples were incubated in SIAH1/SIAH2 monoclonal antibody (Product # MA5-24781). There are two SINA-like E3 ligases, SINA and SINAH, in Drosophila. SINA monoclonal antibody 8G7H12 is highly specific.
Product Details
MA5-24781
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Fruit fly,
Human,
Pig,
Rat,
Zebrafish
Host/Isotype
Mouse
/ IgG1
Class
Monoclonal
Type
Antibody
Clone
8G7H12
Immunogen
Synthetic peptide made to a C-terminal portion of the drosophila SINA protein (within residues 280-331).
Conjugate
Form
Liquid
Concentration
Conc. Not Determined
Storage buffer
tissue culture supernatant
Contains
0.1% sodium azide
Storage conditions
4°C, do not freeze
Shipping conditions
Ambient (domestic); Wet ice (international)
RRID
AB_2717254
Product Specific Information
This is specific for human SIAH 1 and 2 and Drosophila SINA in proliferating cells and stem cells. This does not detect Drosophila SINAH or normal, nondividing cells.
Target Information
SIAH, the human homologue of the Drosophila seven in absentia (sina) gene, is a tumor suppressor protein that is expressed in intestinal epithelium and activated during apoptosis. Human SIAH proteins are produced as two distinct gene products, SIAH-1, and a slightly larger protein SIAH-2, which share a highly conserved C-terminal sequence and differ in their N-terminal regions. SIAH-1 is a 32 kDa protein that contains an N-terminal RING-finger domain, which is required for proteolysis, and a cystein-rich C-terminal domain, which regulates oligomerization and SIAH binding to target proteins. As a tumor suppressor, SIAH-1 binds DCC (deleted in colorectal cancer) and regulates DCC degradation via the ubiquitin-proteasome pathway. SIAH-1 also binds a Bcl-2 related protein, Bag-1, thereby inhibiting cell growth. The majority of SIAH-1 is localized to the nucleus, however a small percentage is detected in the cytoplasm. This nuclear localization suggests that SIAH proteins may interact with other nuclear matrix proteins and DNA.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
Bioinformatics
Protein Aliases: anon-fast-evolving-2H7; CG9949-PA; CG9949-PB; CG9949-PC; E3 ubiquitin-protein ligase SIAH1; E3 ubiquitin-protein ligase SIAH2; E3 ubiquitin-protein ligase sina; EC 6.3.2; FLJ08065; hSIAH1; RING-type E3 ubiquitin transferase SIAH1; RING-type E3 ubiquitin transferase SIAH2; RING-type E3 ubiquitin transferase sina; seven in absenti; seven in absentia; seven in absentia 1A; seven in absentia 2; Seven in absentia homolog 1; Seven in absentia homolog 2; Seven in absentia protein; seven-in-absentia; Siah-1; Siah-1a; Siah-2; sina-PA; sina-PB; sina-PC
Gene Aliases: anon-EST:fe2H7; CG9949; Dmel\CG9949; Dmel_CG9949; hSiah2; HUMSIAH; id:ibd2635; sia; SIAH; SIAH1; SIAH1A; SIAH2; SINA; zgc:56026
UniProt ID: (Human) Q8IUQ4, (Human) O43255, (Rat) Q920M9, (Rat) Q8R4T2, (Zebrafish) Q7ZVG6, (Fruit fly) P21461
Entrez Gene ID: (Human) 6477, (Human) 6478, (Rat) 140941, (Rat) 140593, (Zebrafish) 80955, (Fruit fly) 39884
ubiquitin-protein transferase activity
protein binding
protein C-terminus binding
zinc ion binding
ligase activity
identical protein binding
ubiquitin protein ligase activity
transcription corepressor activity
ubiquitin conjugating enzyme binding
protein homodimerization activity
transferase activity
metal ion binding
protein self-association
protein polyubiquitination
ubiquitin-dependent protein catabolic process
apoptotic process
cell cycle
small GTPase mediated signal transduction
axon guidance
regulation of protein ubiquitination
protein ubiquitination involved in ubiquitin-dependent protein catabolic process
negative regulation of apoptotic process
negative regulation of cysteine-type endopeptidase activity involved in apoptotic process
proteasome-mediated ubiquitin-dependent protein catabolic process
cellular protein catabolic process
cellular protein metabolic process
negative regulation of canonical Wnt signaling pathway
negative regulation of nucleic acid-templated transcription
negative regulation of extrinsic apoptotic signaling pathway
spermatogenesis
nervous system development
anatomical structure morphogenesis
protein catabolic process
protein destabilization
positive regulation of apoptotic process
neuron apoptotic process
positive regulation of intrinsic apoptotic signaling pathway
proteolysis
sensory organ development
R7 cell fate commitment
proteasomal protein catabolic process
protein ubiquitination
regulation of R7 cell differentiation
male meiosis I
multicellular organism development
post-embryonic development
cell differentiation
regulation of multicellular organism growth
Performance Guarantee
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