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SIGLEC12 Antibody (PA5-47780) in IHC (F)
Immunohistochemical analysis of SIGLEC12 in perfusion fixed frozen sections of mouse spleen. Samples were incubated with SIGLEC12 polyclonal antibody (Product # PA5-47780) using a dilution of 1.7 µg/mL overnight at 4 °C followed by NorthernLights™ 557-conjugated Anti-Goat IgG Secondary Antibody (red) and counterstained with DAPI (blue). Specific staining was localized to plasma membrane.
Product Details
PA5-47780
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Mouse
Host/Isotype
Goat
/ IgG
Class
Polyclonal
Type
Antibody
Immunogen
Mouse myeloma cell line NS0-derived recombinant mouse Siglec-E Gln20-Phe355
Conjugate
Form
Lyophilized
Concentration
0.2 mg/mL
Purification
Antigen affinity chromatography
Storage buffer
PBS with 5% trehalose
Contains
No Preservative
Storage conditions
-20°C, Avoid Freeze/Thaw Cycles
Shipping conditions
Ambient (domestic); Wet ice (international)
RRID
AB_2606303
Product Specific Information
In direct ELISAs, less than 3% cross-reactivity with recombinant human (rh) Siglec-6, rhSiglec-7, and rhSiglec-9 is observed.
Reconstitute at 0.2 mg/mL in sterile PBS.
Target Information
Sialic acid-binding immunoglobulin-like lectins are a family of cell surface proteins belonging to the immunoglobulin superfamily. They mediate protein-carbohydrate interactions by selectively binding to different sialic acid moieties present on glycolipids and glycoproteins. This gene encodes a member of the SIGLEC3-like subfamily of SIGLECs. Members of this subfamily are characterized by an extracellular V-set immunoglobulin-like domain followed by two C2-set immunoglobulin-like domains, and the cytoplasmic tyrosine-based motifs ITIM and SLAM-like. The encoded protein, upon tyrosine phosphorylation, has been shown to recruit the Src homology 2 domain-containing protein-tyrosine phosphatases SHP1 and SHP2. It has been suggested that the protein is involved in the negative regulation of macrophage signaling by functioning as an inhibitory receptor. This gene is located in a cluster with other SIGLEC3-like genes on 19q13.4. Alternatively spliced transcript variants encoding distinct isoforms have been described for this gene.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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Bioinformatics
Protein Aliases: MIS; Myeloid inhibitory siglec; sialic acid binding Ig-like lectin 5; Sialic acid-binding Ig-like lectin 12; Sialic acid-binding Ig-like lectin 5; Sialic acid-binding Ig-like lectin E; Sialic acid-binding Ig-like lectin-like 1; sialic acid-binding immunoglobulin-like lectin E; Siglec; Siglec-12; Siglec-5; Siglec-E; Siglec-L1; SIGLEC-like 1
Gene Aliases: Cd170; mSiglec-E; Siglec12; Siglec5; Siglec9; Siglece; Siglecl1
UniProt ID: (Mouse) Q91Y57
Entrez Gene ID: (Mouse) 83382
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