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Invitrogen
SIL Polyclonal Antibody
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FIGURE: 1 / 3
SIL Antibody (PA5-115974) in ICC/IF
Immunocytochemistry analysis of SIL in HeLa cells. Samples were treated with PFA, permeabilized in 0.1% Triton X-100, blocked in 10% serum (45 min at 25°C), and incubated with polyclonal antibody (Product # PA5-115974) at a dilution of 1:200 (1 hr at 37°C). Secondary staining was applied with mouse anti-beta tubulin; AlexaFluor 594 conjugated goat anti-rabbit IgG (Red); AlexaFluor 88 conjugated goat anti-mouse IgG (Green) and DAPI (blue) using a dilution of 1:200 (1 hr at 37°C).
Product Details
PA5-115974
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Human,
Mouse,
Rat
Host/Isotype
Rabbit
/ IgG
Class
Polyclonal
Type
Antibody
Immunogen
A synthesized peptide derived from human STIL(Accession Q15468), corresponding to amino acid residues E1236-L1286.
Conjugate
Form
Liquid
Concentration
1 mg/mL
Purification
Affinity chromatography
Storage buffer
PBS with 50% glycerol
Contains
0.02% sodium azide
Storage conditions
-20°C
Shipping conditions
Ambient (domestic); Wet ice (international)
RRID
AB_2900608
Product Specific Information
Antibody detects endogenous levels of total STIL.
Target Information
Plays an essential role in early embryonic development.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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Bioinformatics
Protein Aliases: SCL-interrupting locus protein; SCL-interrupting locus protein homolog; Scl/Tal1 interrupting locus; TAL-1-interrupting locus protein; Tal1 interrupting locus
Gene Aliases: MCPH7; SIL; STIL
UniProt ID: (Human) Q15468, (Mouse) Q60988
Entrez Gene ID: (Human) 6491, (Mouse) 20460, (Rat) 313506
embryonic axis specification
in utero embryonic development
neural tube closure
heart looping
mitotic spindle organization
smoothened signaling pathway
multicellular organism development
determination of left/right symmetry
neural tube development
forebrain development
notochord development
floor plate development
multicellular organism growth
negative regulation of apoptotic process
positive regulation of cyclin-dependent protein serine/threonine kinase activity
centrosome duplication
cilium morphogenesis
mitotic spindle checkpoint
protein localization to centrosome
cell proliferation
regulation of centriole replication
biological_process
Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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