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          • Primary Antibodies ›
          • SIRT3 Antibodies

          Invitrogen

          SIRT3 Recombinant Rabbit Monoclonal Antibody (5T9J5)

          View all (28) SIRT3 antibodies

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          Cite SIRT3 Recombinant Rabbit Monoclonal Antibody (5T9J5)

          • Antibody Testing Data (3)
          SIRT3 Antibody in Western Blot (WB)
          Group 53 Created with Sketch.
          SIRT3 Antibody in Western Blot (WB)
          Group 53 Created with Sketch.

          FIGURE: 1 / 3

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          SIRT3 Antibody (MA5-51256) in WB

          Western blot analysis of SIRT3 in extracts of various cell lines. Samples were incubated with SIRT3 Recombinant monoclonal antibody (Product # MA5-51256) using a dilution of 1:1,000 followed by HRP Goat Anti-Rabbit IgG (H+L) secondary antibody at a dilution of 1:10,000. Lysates/proteins: 25 µg per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Enhanced Kit. Exposure time: 60s. {{ $ctrl.currentElement.advancedVerification.fullName }} validation info. View more
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          SIRT3 Antibody in Western Blot (WB)
          SIRT3 Antibody in Western Blot (WB)
          SIRT3 Antibody in Western Blot (WB)

          Product Details

          MA5-51256

          Applications
          Tested Dilution
          Publications

          Western Blot (WB)

          1:1,000-1:2,000
          -

          ELISA (ELISA)

          1 µg/mL
          -
          Product Specifications

          Species Reactivity

          Human, Mouse, Rat

          Host/Isotype

          Rabbit / IgG

          Expression System

          HEK293 cells

          Class

          Recombinant Monoclonal

          Type

          Antibody

          Clone

          5T9J5

          Immunogen

          A synthetic peptide corresponding to a sequence within amino acids 300-399 of human SIRT3 (NP_036371.1).
          View immunogen

          Conjugate

          Unconjugated Unconjugated Unconjugated

          Form

          Liquid

          Concentration

          1 mg/mL

          Purification

          Affinity chromatography

          Storage buffer

          PBS, pH 7.3, with 0.05% BSA, 50% glycerol

          Contains

          0.05% ProClin 300

          Storage conditions

          -20°C, Avoid Freeze/Thaw Cycles

          Shipping conditions

          Ambient (domestic); Wet ice (international)

          RRID

          AB_3093917

          Product Specific Information

          Immunogen sequence: QRFLLHVVDF PMADLLLILG TSLEVEPFAS LTEAVRSSVP RLLINRDLVG PLAWHPRSRD VAQLGDVVHG VESLVELLGW TEEMRDLVQR ETGKLDGPDK

          Target Information

          SIRT3 is a member of the sirtuin family of proteins, homologs to the yeast Sir2 protein. Members of the sirtuin family are characterized by a sirtuin core domain and grouped into four classes. The functions of human sirtuins have not yet been determined; however, yeast sirtuin proteins are known to regulate epigenetic gene silencing and suppress recombination of rDNA. Studies suggest that the human sirtuins may function as intracellular regulatory proteins with mono-ADP-ribosyltransferase activity. The SIRT3 is included in class I of the sirtuin family.

          For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

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          Cite this product

          Bioinformatics

          Protein Aliases: HGNC:14931; hSIRT3; mitochondrial nicotinamide adenine dinucleotide-dependent deacetylase; mitochondrial protein lysine deacetylase; mSIR2L3; NAD-dependent deacetylase sirtuin-3; NAD-dependent deacetylase sirtuin-3, mitochondrial; NAD-dependent protein deacetylase sirtuin-3; NAD-dependent protein deacetylase sirtuin-3, mitochondrial; NAD-dependent protein delactylase sirtuin-3; Regulatory protein SIR2 homolog 3; silent mating type information regulation 2, (S.cerevisiae, homolog)-like 3; silent mating type information regulation 2, S.cerevisiae, homolog 3; sir2-like 3; SIR2-like protein 3; SIRT3L mitochondrial; sirtuin type 3

          View more View less

          Gene Aliases: 2310003L23Rik; AI848213; SIR2L3; SIRT3

          View more View less

          UniProt ID: (Human) Q9NTG7, (Mouse) Q8R104

          View more View less

          Entrez Gene ID: (Human) 23410, (Mouse) 64384, (Rat) 293615

          View more View less

          Function(s)
          NAD+ ADP-ribosyltransferase activity protein binding zinc ion binding NAD-dependent histone deacetylase activity (H3-K14 specific) NAD+ binding histone deacetylase activity protein-N-terminal asparagine amidohydrolase activity UDP-3-O-[3-hydroxymyristoyl] N-acetylglucosamine deacetylase activity hydrolase activity hydrolase activity, acting on carbon-nitrogen (but not peptide) bonds, in linear amides iprodione amidohydrolase activity (3,5-dichlorophenylurea)acetate amidohydrolase activity enzyme binding 4'-(2-hydroxyisopropyl)phenylurea amidohydrolase activity didemethylisoproturon amidohydrolase activity N-isopropylacetanilide amidohydrolase activity N-cyclohexylformamide amidohydrolase activity isonicotinic acid hydrazide hydrolase activity cis-aconitamide amidase activity gamma-N-formylaminovinylacetate hydrolase activity N2-acetyl-L-lysine deacetylase activity O-succinylbenzoate synthase activity indoleacetamide hydrolase activity N-acetylcitrulline deacetylase activity metal ion binding N-acetylgalactosamine-6-phosphate deacetylase activity NAD binding diacetylchitobiose deacetylase activity chitooligosaccharide deacetylase activity
          Process(es)
          protein ADP-ribosylation protein deacetylation mitochondrion organization aerobic respiration peptidyl-lysine deacetylation histone H3 deacetylation negative regulation of peptidyl-lysine acetylation positive regulation of insulin secretion negative regulation of ERK1 and ERK2 cascade negative regulation of reactive oxygen species metabolic process aging
          It has to be done as per old AB suggested Products section.
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          If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*

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