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          • Primary Antibodies ›
          • SKAP55 Antibodies

          Invitrogen

          SKAP55 Polyclonal Antibody

          View all (5) SKAP55 antibodies

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          Cite SKAP55 Polyclonal Antibody

          • Antibody Testing Data (1)
          SKAP55 Antibody in Western Blot (WB)
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          SKAP55 Antibody in Western Blot (WB)
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          FIGURE: 1 / 1

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          SKAP55 Antibody (PA5-96242) in WB

          Western blot analysis of SKAP55 in extracts of various cell lines. Samples were incubated with SKAP55 Polyclonal antibody (Product # PA5-96242) using a dilution of 1:3,000, followed by HRP Goat Anti-Rabbit IgG (H+L) at a dilution of 1:10,000. Lysates/proteins: 25 µg per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 90s. {{ $ctrl.currentElement.advancedVerification.fullName }} validation info. View more
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          SKAP55 Antibody in Western Blot (WB)

          Product Details

          PA5-96242

          Applications
          Tested Dilution
          Publications

          Western Blot (WB)

          1:500-1:2,000
          -

          ELISA (ELISA)

          1 µg/mL
          -
          Product Specifications

          Species Reactivity

          Mouse, Rat

          Host/Isotype

          Rabbit / IgG

          Class

          Polyclonal

          Type

          Antibody

          Immunogen

          Recombinant fusion protein containing a sequence corresponding to amino acids 1-358 of human SKAP1 (NP_0010685671)
          View immunogen

          Conjugate

          Unconjugated Unconjugated Unconjugated

          Form

          Liquid

          Concentration

          3.82 mg/mL

          Purification

          Affinity Chromatography

          Storage buffer

          PBS, pH 7.3, with 50% glycerol

          Contains

          0.02% sodium azide

          Storage conditions

          -20°C, Avoid Freeze/Thaw Cycles

          Shipping conditions

          Wet ice

          RRID

          AB_2808044

          Product Specific Information

          Immunogen sequence: MQAAALPEEI RWLLEDAEEF LAEGLRNENL SAVARDHRDH ILRGFQQIKA RYYWDFQPQG GDIGQDSSDD NHSGTLGLSL TSDAPFLSDY QDEGMEDIVK GAQELDNVIK QGYLEKKSKD HSFFGSEWQK RWCVVSRGLF YYYANEKSKQ PKGTFLIKGY GVRMAPHLRR DSKKESCFEL TSQDRRSYEF TATSPAEARD WVDQISFLLK DLSSLTIPYE EDEEEEEKEE TYDDIDGFDS PSCGSQCRPT ILPGSVGIKE PTEEKEEEDI YEVLPDEEHD LEEDESGTRR KGDYASYYQG LWDCHGDQPD ELSFQRGDLI RILSKEYNMY GWWVGELNSL VGIVPKEYLT TAFEVEER; Positive Samples: Mouse thymus, Rat thymus; Cellular Location: Cell membrane, Cytoplasm, Nucleus

          Target Information

          This gene encodes a T cell adaptor protein, a class of intracellular molecules with modular domains capable of recruiting additional proteins but that exhibit no intrinsic enzymatic activity. The encoded protein contains a unique N-terminal region followed by a PH domain and C-terminal SH3 domain. Along with the adhesion and degranulation-promoting adaptor protein, the encoded protein plays a critical role in inside-out signaling by coupling T-cell antigen receptor stimulation to the activation of integrins.

          For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

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          Cite this product

          Bioinformatics

          Protein Aliases: ortholog of human src family associated phosphoprotein 1 SCAP1; pp55; SKAP-55; SKAP55 adapter protein; SKAP55 adaptor protein; src family associated phosphoprotein 1; Src family-associated phosphoprotein 1; Src kinase-associated phosphoprotein 1; Src kinase-associated phosphoprotein of 55 kDa

          View more View less

          Gene Aliases: 1700091G21Rik; Scap1; Skap-55; Skap1; Skap55

          View more View less

          UniProt ID: (Mouse) Q3UUV5, (Rat) Q4V7G1

          View more View less

          Entrez Gene ID: (Mouse) 78473, (Rat) 286975

          View more View less

          Function(s)
          protein binding protein kinase binding protein phosphatase binding protein complex binding SH2 domain binding
          Process(es)
          immune system process positive regulation of cell adhesion positive regulation of transcription, DNA-templated positive regulation of transcription from RNA polymerase II promoter T cell receptor signaling pathway protein localization to plasma membrane
          It has to be done as per old AB suggested Products section.
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          If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*

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