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SKIL Polyclonal Antibody

SKIL Antibody in Immunocytochemistry (ICC/IF)

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SKIL Antibody (19218-1-AP) in ICC/IF

Immunofluorescent analysis of (4% PFA) fixed HepG2 cells using SKIL antibody (Product # 19218-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

SKIL Antibody in Immunohistochemistry (Paraffin) (IHC (P))

SKIL Antibody in Immunoprecipitation (IP)

Product Image Gallery

Product Details

19218-1-AP

Applications

Tested Dilution

Publications

Western Blot (WB)

1:500-1:2,000

View 8 publications 8 publications

Immunohistochemistry (IHC)

View 1 publication 1 publication

Immunohistochemistry (Paraffin) (IHC (P))

1:200

Immunocytochemistry (ICC/IF)

1:200

View 2 publications 2 publications

Immunoprecipitation (IP)

0.5-4.0 µg

View 2 publications 2 publications

Not applicable (N/A)

View 1 publication 1 publication

Product Specifications

Species Reactivity

Human

Published species

Human

Host/Isotype

Rabbit / IgG

Class

Polyclonal

Type

Antibody

Immunogen

SKIL Fusion Protein Ag5282 (335-684 aa encoded by BC059386)

View immunogen

Conjugate

Unconjugated Unconjugated Unconjugated

Form

Liquid

Concentration

0.17 mg/mL

Purification

Antigen affinity chromatography

Storage buffer

PBS, pH 7.3, with 50% glycerol

Contains

0.02% sodium azide

Storage conditions

-20°C

Shipping conditions

Wet ice (domestic); Dry ice (international)

Product Specific Information

Immunogen sequence: YLGTPEEKK LKIILEEMKE KFSMRSGKRN QSKTDAPSGM ELQSWYPVIK QEGDHVSQTH SFLHPSYYLY MCDKVVAPNV SLTSAVSQSK ELTKTEASKS ISRQSEKAHS SGKLQKTVSY PDVSLEEQEK MDLKTSRELC SRLDASISNN STSKRKSESA TCNLVRDINK VGIGLVAAAS SPLLVKDVIC EDDKGKIMEE VMRTYLKQQE KLNLILQKKQ QLQMEVKMLS SSKSMKELTE EQQNLQKELE SLQNEHAQRM EEFYVEQKDL EKKLEQIMKQ KCTCDSNLEK DKEAEYAGQL AELRQRLDHA EADRQELQDE LRQEREARQK LEMMIKELKL QILKSSKTAK E (335-684 aa encoded by BC059386)

Target Information

TGF-beta is a ubiquitously expressed cytokine that signals through the Smad protein family to regulate numerous cellular processes such as embryonic development and tumorigenesis. This signaling is negatively regulated by Ski, the mammalian homolog of the transforming protein of an avian retrovirus that induces oncogenic transformation of chicken embryo cells, and the related protein SnoN. Like Ski, SnoN functions by binding to the Smad proteins and preventing their phosphorylation, thereby inhibiting their ability to bind DNA and activate the transcription of downstream genes. SnoN is located primarily in the nucleus in cancer tissues or cells, but in the cytoplasm in normal tissues or primary epithelial cells.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

Product References

The UAS thioredoxin-like domain of UBXN7 regulates E3 ubiquitin ligase activity of RNF111/Arkadia.

BMC biology

Amhaz S,Boëda B,Chouchène M,Colasse S,Dingli F,Loew D,Henri J,Prunier C,Levy L

Fri Apr 07 00:00:00 UTC 2023

BMP-7 ameliorates partial epithelial-mesenchymal transition by restoring SnoN protein level via Smad1/5 pathway in diabetic kidney disease.

Cell death & disease

Peng W,Zhou X,Xu T,Mao Y,Zhang X,Liu H,Liang L,Liu L,Liu L,Xiao Y,Zhang F,Li S,Shi M,Zhou Y,Tang L,Wang Y,Guo B

Mon Mar 21 00:00:00 UTC 2022

BMP-7 ameliorates partial epithelial-mesenchymal transition by restoring SnoN protein level via Smad1/5 pathway in diabetic kidney disease.

Cell death & disease

Peng W,Zhou X,Xu T,Mao Y,Zhang X,Liu H,Liang L,Liu L,Liu L,Xiao Y,Zhang F,Li S,Shi M,Zhou Y,Tang L,Wang Y,Guo B

Mon Mar 21 00:00:00 UTC 2022

SKI knockdown suppresses apoptosis and extracellular matrix degradation of nucleus pulposus cells via inhibition of the Wnt/β-catenin pathway and ameliorates disc degeneration.

Apoptosis : an international journal on programmed cell death

Wu ZL,Chen YJ,Zhang GZ,Xie QQ,Wang KP,Yang X,Liu TC,Wang ZQ,Zhao GH,Zhang HH

Tue Feb 01 00:00:00 UTC 2022

SKI knockdown suppresses apoptosis and extracellular matrix degradation of nucleus pulposus cells via inhibition of the Wnt/β-catenin pathway and ameliorates disc degeneration.

Apoptosis : an international journal on programmed cell death

Wu ZL,Chen YJ,Zhang GZ,Xie QQ,Wang KP,Yang X,Liu TC,Wang ZQ,Zhao GH,Zhang HH

Tue Feb 01 00:00:00 UTC 2022

SKI knockdown suppresses apoptosis and extracellular matrix degradation of nucleus pulposus cells via inhibition of the Wnt/β-catenin pathway and ameliorates disc degeneration.

Apoptosis : an international journal on programmed cell death

Wu ZL,Chen YJ,Zhang GZ,Xie QQ,Wang KP,Yang X,Liu TC,Wang ZQ,Zhao GH,Zhang HH

Tue Feb 01 00:00:00 UTC 2022

Quantitative Ubiquitylome Analysis Reveals the Specificity of RNF111/Arkadia E3 Ubiquitin Ligase for its Degradative Substrates SKI and SKIL/SnoN in TGF-β Signaling Pathway.

Molecular & cellular proteomics : MCP

Laigle V,Dingli F,Amhaz S,Perron T,Chouchène M,Colasse S,Petit I,Poullet P,Loew D,Prunier C,Levy L

Fri Mar 11 00:00:00 UTC 2022

Inhibition of MAD2B alleviates venous neointimal formation by suppressing VSMCs proliferation and migration.

FASEB journal : official publication of the Federation of American Societies for Experimental Biology

Song AN,Yin XJ,Gao P,Tang H,Meng XF,Zhang C

Mon Nov 01 00:00:00 UTC 2021

PIAS1 and TIF1γ collaborate to promote SnoN SUMOylation and suppression of epithelial-mesenchymal transition.

Cell death and differentiation

Chanda A,Ikeuchi Y,Karve K,Sarkar A,Chandhoke AS,Deng L,Bonni A,Bonni S

Fri Jan 01 00:00:00 UTC 2021

Structure-Guided Design of a Small-Molecule Activator of Sirtuin-3 that Modulates Autophagy in Triple Negative Breast Cancer.

Journal of medicinal chemistry

Zhang J,Zou L,Shi D,Liu J,Zhang J,Zhao R,Wang G,Zhang L,Ouyang L,Liu B

Thu Oct 14 00:00:00 UTC 2021

SKIL facilitates tumorigenesis and immune escape of NSCLC via upregulating TAZ/autophagy axis.

Cell death & disease

Ma F,Ding MG,Lei YY,Luo LH,Jiang S,Feng YH,Liu XL

Wed Dec 02 00:00:00 UTC 2020

The Transcriptional Regulator SnoN Promotes the Proliferation of Cerebellar Granule Neuron Precursors in the Postnatal Mouse Brain.

The Journal of neuroscience : the official journal of the Society for Neuroscience

Chen X,Chanda A,Ikeuchi Y,Zhang X,Goodman JV,Reddy NC,Majidi SP,Wu DY,Smith SE,Godec A,Oldenborg A,Gabel HW,Zhao G,Bonni S,Bonni A

Wed Jan 02 00:00:00 UTC 2019

MDM2 is implicated in high-glucose-induced podocyte mitotic catastrophe via Notch1 signalling.

Journal of cellular and molecular medicine

Tang H,Lei CT,Ye C,Gao P,Wan C,Chen S,He FF,Wang YM,Su H,Zhang C

Fri Dec 01 00:00:00 UTC 2017

MDM2 is implicated in high-glucose-induced podocyte mitotic catastrophe via Notch1 signalling.

Journal of cellular and molecular medicine

Tang H,Lei CT,Ye C,Gao P,Wan C,Chen S,He FF,Wang YM,Su H,Zhang C

Fri Dec 01 00:00:00 UTC 2017

De novo GLI3 mutation in esophageal atresia: reproducing the phenotypic spectrum of Gli3 defects in murine models.

Biochimica et biophysica acta

Yang L,Shen C,Mei M,Zhan G,Zhao Y,Wang H,Huang G,Qiu Z,Lu W,Zhou W

Mon Sep 01 00:00:00 UTC 2014

De novo GLI3 mutation in esophageal atresia: reproducing the phenotypic spectrum of Gli3 defects in murine models.

Biochimica et biophysica acta

Yang L,Shen C,Mei M,Zhan G,Zhao Y,Wang H,Huang G,Qiu Z,Lu W,Zhou W

Mon Sep 01 00:00:00 UTC 2014

Bioinformatics

Protein Aliases: SKI-like oncogene; Ski-like protein; Ski-related oncogene; ski-related oncogene snoN; Ski-related protein

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Gene Aliases: SKIL; SNO; SnoA; SnoI; SnoN

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UniProt ID: (Human) P12757

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Entrez Gene ID: (Human) 6498

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Function(s)

RNA polymerase II core promoter proximal region sequence-specific DNA binding transcriptional repressor activity, RNA polymerase II core promoter proximal region sequence-specific binding chromatin binding protein binding SMAD binding

Process(es)

negative regulation of transcription from RNA polymerase II promoter blastocyst formation lymphocyte homeostasis cell cycle arrest transforming growth factor beta receptor signaling pathway spermatogenesis negative regulation of transforming growth factor beta receptor signaling pathway response to cytokine regulation of apoptotic process negative regulation of cell differentiation positive regulation of axonogenesis lens fiber cell differentiation positive regulation of extrinsic apoptotic signaling pathway via death domain receptors positive regulation of intrinsic apoptotic signaling pathway in response to DNA damage

Performance Guarantee

If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*

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SKIL Polyclonal Antibody

SKIL Antibody (19218-1-AP) in ICC/IF

Product Details

19218-1-AP

Western Blot (WB)

Immunohistochemistry (IHC)

Immunohistochemistry (Paraffin) (IHC (P))

Immunocytochemistry (ICC/IF)

Immunoprecipitation (IP)

Not applicable (N/A)

Species Reactivity

Published species

Host/Isotype

Class

Type

Immunogen

Conjugate

Form

Concentration

Purification

Storage buffer

Contains

Storage conditions

Shipping conditions

Product Specific Information

Target Information

Bioinformatics

Performance Guarantee

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