Invitrogen

SKP1 Monoclonal Antibody (4E11)

SKP1 Antibody in Immunocytochemistry (ICC/IF)

FIGURE: 1 / 9

SKP1 Antibody (MA5-15928) in ICC/IF

Immunofluorescence analysis of SKP1 was performed using 70% confluent log phase MCF-7 cells. The cells were fixed with 4% paraformaldehyde for 10 minutes, permeabilized with 0.1% Triton™ X-100 for 15 minutes, and blocked with 1% BSA for 1 hour at room temperature. The cells were labeled with SKP1 Mouse Monoclonal Antibody (4E11) (Product # MA5-15928) at 1:100 dilution in 0.1% BSA, incubated at 4 degree Celsius overnight and then labeled with Goat anti-Mouse IgG (H+L) Superclonal™ Secondary Antibody, Alexa Fluor® 488 conjugate (Product # A2... View More

SKP1 Antibody in Immunohistochemistry (Paraffin) (IHC (P))

Product Image Gallery

SKP1 Antibody — Antibody specificity was demonstrated by siRNA mediated knockdown of target protein. U-87 MG cells were transfected with SKP1 siRNA and decrease in signal was observed in Western Blot using Anti-SKP1 Monoclonal Antibody (Product # MA5-15928). {KD}

Product Details

MA5-15928

Applications

Tested Dilution

Publications

Western Blot (WB)

1:500-1:2,000

View 3 publications 3 publications

Immunohistochemistry (Paraffin) (IHC (P))

1:200-1:1,000

Immunocytochemistry (ICC/IF)

1:200-1:1,000

Flow Cytometry (Flow)

1:200-1:400

ELISA (ELISA)

1:10,000

Product Specifications

Species Reactivity

Human, Mouse, Non-human primate

Published species

Human

Host/Isotype

Mouse / IgG1

Class

Monoclonal

Type

Antibody

Clone

4E11

Immunogen

Purified recombinant fragment of human SKP1 expressed in E. Coli.

Conjugate

Unconjugated Unconjugated Unconjugated

Form

Liquid

Concentration

Conc. Not Determined

Storage buffer

ascites

Contains

0.03% sodium azide

Storage conditions

Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.

Shipping conditions

Ambient (domestic); Wet ice (international)

AB_11153724

Product Specific Information

MA5-15928 targets SKP1 in indirect ELISA, FACS, IF, IHC, and WB applications and shows reactivity with Human samples.

The MA5-15928 immunogen is purified recombinant fragment of human SKP1 expressed in E. Coli. .

MA5-15928 detects SKP1 which has a predicted molecular weight of approximately 19kDa.

Target Information

This gene encodes a component of SCF complexes, which are composed of this protein, cullin 1, a ring-box protein, and one member of the F-box family of proteins. This protein binds directly to the F-box motif found in F-box proteins. SCF complexes are involved in the regulated ubiquitination of specific protein substrates, which targets them for degradation by the proteasome. Specific F-box proteins recognize different target protein(s), and many specific SCF substrates have been identified including regulators of cell cycle progression and development. Studies have also characterized the protein as an RNA polymerase II elongation factor. Alternative splicing of this gene results in two transcript variants. A related pseudogene has been identified on chromosome 7.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

Product References

An innate pathogen sensing strategy involving ubiquitination of bacterial surface proteins.

Science advances

Apte S,Bhutda S,Ghosh S,Sharma K,Barton TE,Dibyachintan S,Sahay O,Roy S,Sinha AR,Adicherla H,Rakshit J,Tang S,Datey A,Santra S,Joseph J,Sasidharan S,Hammerschmidt S,Chakravortty D,Oggioni MR,Santra MK,Neill DR,Banerjee A

MA5-15928 was used in Western Blot to conclude that exploitation of this generic pathogen sensing strategy allows conservation of host resources and boosts antimicrobial immunity.

Wed Mar 22 00:00:00 UTC 2023

Differential sensitivity of acute myeloid leukemia cells to daunorubicin depends on P2X7A versus P2X7B receptor expression.

Cell death & disease

Pegoraro A,Orioli E,De Marchi E,Salvestrini V,Milani A,Di Virgilio F,Curti A,Adinolfi E

MA5-15928 was used in Western Blotting to investigate the expression of P2X7RA and P2X7RB in samples obtained from myelodysplastic syndrome and acute myeloid leukemia (AML) untreated subjects or AML patients in relapse or remission after chemotherapy.

Sun Oct 18 00:00:00 UTC 2020

Cand1-Mediated Adaptive Exchange Mechanism Enables Variation in F-Box Protein Expression.

Molecular cell

Liu X,Reitsma JM,Mamrosh JL,Zhang Y,Straube R,Deshaies RJ

MA5-15928 was used in Western Blot to develop a mathematical model for how dynamical assembly of Skp1⋅Cul1⋅F-box (SCF) complexes is controlled and how this cycle is coupled to degradation of an SCF substrate.

Thu Mar 01 00:00:00 UTC 2018

Bioinformatics

Protein Aliases: cyclin A/CDK2-associated p19; cyclin A/CDK2-associated protein p19; Cyclin-A/CDK2-associated protein p19; MGC34403; OCP-2; OCP-II; OCP-II protein; Organ of Corti protein 2; Organ of Corti protein II; p19A; p19skp1; polypeptide 1-like; RNA polymerase II elongation factor-like protein; RNA polymerase II elongation factor-like protein OCP2; S-phase kinase-associated protein 1; S-phase kinase-associated protein 1A; SIII; Transcription elongation factor B polypeptide 1-like

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Gene Aliases: 15kDa; 2610043E24Rik; 2610206H23Rik; EMC19; OCP-II; OCP2; p19A; p19Skp1; SKP1; SKP1A; TCEB1L

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UniProt ID: (Human) P63208, (Mouse) Q9WTX5

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Entrez Gene ID: (Human) 6500, (Mouse) 21402

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Function(s)

ubiquitin-protein transferase activity protein binding beta-catenin binding ubiquitin protein ligase activity cullin family protein binding

Process(es)

ubiquitin-dependent protein catabolic process protein ubiquitination SCF-dependent proteasomal ubiquitin-dependent protein catabolic process histone H2A monoubiquitination G2/M transition of mitotic cell cycle protein polyubiquitination stimulatory C-type lectin receptor signaling pathway Wnt signaling pathway NIK/NF-kappaB signaling Fc-epsilon receptor signaling pathway proteasome-mediated ubiquitin-dependent protein catabolic process T cell receptor signaling pathway stress-activated MAPK cascade positive regulation of ubiquitin-protein ligase activity involved in regulation of mitotic cell cycle transition

Performance Guarantee

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SKP1 Monoclonal Antibody (4E11)

SKP1 Antibody (MA5-15928) in ICC/IF

Product Details

MA5-15928

Western Blot (WB)

Immunohistochemistry (Paraffin) (IHC (P))

Immunocytochemistry (ICC/IF)

Flow Cytometry (Flow)

ELISA (ELISA)

Species Reactivity

Published species

Host/Isotype

Class

Type

Clone

Immunogen

Conjugate

Form

Concentration

Storage buffer

Contains

Storage conditions

Shipping conditions

RRID

Product Specific Information

Target Information

Bioinformatics

Performance Guarantee

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