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          • Primary Antibodies ›
          • SKP2 Antibodies

          Invitrogen

          SKP2 Monoclonal Antibody (6G9D10)

          View all (17) SKP2 antibodies

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          Cite SKP2 Monoclonal Antibody (6G9D10)

          • Antibody Testing Data (3)
          SKP2 Antibody in Western Blot (WB)
          Group 53 Created with Sketch.
          SKP2 Antibody in Western Blot (WB)
          Group 53 Created with Sketch.

          FIGURE: 1 / 3

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          SKP2 Antibody (MA5-15470) in WB

          Western blot was performed using Anti-SKP2 Monoclonal Antibody (6G9D10) (Product # MA5-15470) and a 52 kDa band corresponding to SKP2 was observed across cell lines. Whole cell extracts (30 µg lysate) of LNCaP (Lane 1), PC-3 (Lane 2), BeWo (Lane 3) were electrophoresed using NuPAGE™ 4-12% Bis-Tris Protein Gel (Product # NP0321BOX). Resolved proteins were then transferred onto a nitrocellulose membrane (Product # IB23002) by iBlot® 2 Dry Blotting System (Product # IB21001). The blot was probed with the primary antibody (1:1000 dilution) and... View More {{ $ctrl.currentElement.advancedVerification.fullName }} validation info. View more
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          SKP2 Antibody in Western Blot (WB)
          SKP2 Antibody in Western Blot (WB)
          SKP2 Antibody in Western Blot (WB)

          Product Details

          MA5-15470

          Applications
          Tested Dilution
          Publications

          Western Blot (WB)

          1:500-1:2,000
          -

          ELISA (ELISA)

          1:10,000
          -
          Product Specifications

          Species Reactivity

          Human

          Host/Isotype

          Mouse / IgG1

          Class

          Monoclonal

          Type

          Antibody

          Clone

          6G9D10

          Immunogen

          Purified recombinant fragment of SKP2 (aa1-130) expressed in E. Coli.
          View immunogen

          Conjugate

          Unconjugated Unconjugated Unconjugated

          Form

          Liquid

          Concentration

          Conc. Not Determined

          Storage buffer

          ascites

          Contains

          0.03% sodium azide

          Storage conditions

          Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.

          Shipping conditions

          Ambient (domestic); Wet ice (international)

          RRID

          AB_10985066

          Product Specific Information

          MA5-15470 targets SKP2 in indirect ELISA, WB applications and shows reactivity with Human samples.

          The MA5-15470 immunogen is purified recombinant fragment of SKP2 (aa1-130) expressed in E. Coli.

          Target Information

          Skp2 (S-phase Kinase-associated Protein 2) belongs to the family of F-box proteins that interact with the Cyclin A-Cdk2 complex. Skp2 is essential for the G1-S transition in both transformed cells and diploid fibroblasts. Biochemical and genetic experiments have demonstrated that Skp2 is required for the ubiquitination and consequent degradation of p27 in cultured mammalian cells and in vitro reconstitution assays. In normal tissues, Skp2 is expressed in tonsil and placenta. In tumor tissues, Skp2 is expressed widely, in some colon, prostate, pancreas, and skin cancers, with high expression in lung, breast, ovarian, and endometrial cancers. Research suggests that Skp2 plays a role in oncogenesis, particularly in the pathogenesis of lymphomas and breast cancers.

          For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

          References (0)

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          Cite this product

          Bioinformatics

          Protein Aliases: CDK2/cyclin A-associated protein p45; Cyclin-A/CDK2-associated protein p45; F-box protein Skp2; F-box/LRR-repeat protein 1; MGC1366; OTTHUMP00000221389; p45skp2; S-phase kinase-associated protein 2; S-phase kinase-associated protein 2 (p45)

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          Gene Aliases: FBL1; FBXL1; FLB1; p45; SKP2

          View more View less

          UniProt ID: (Human) Q13309

          View more View less

          Entrez Gene ID: (Human) 6502

          View more View less

          Function(s)
          ubiquitin-protein transferase activity protein binding identical protein binding ubiquitin protein ligase activity
          Process(es)
          G1/S transition of mitotic cell cycle G2/M transition of mitotic cell cycle protein polyubiquitination cell proliferation anaphase-promoting complex-dependent catabolic process positive regulation of intracellular estrogen receptor signaling pathway regulation of apoptotic process positive regulation of smooth muscle cell proliferation regulation of cell cycle cellular response to cell-matrix adhesion positive regulation of protein polyubiquitination
          It has to be done as per old AB suggested Products section.
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          Performance Guarantee

          If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*

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