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SLC12A3 Antibody (PA5-42408) in WB
Western blot was performed using Anti-SLC12A3 Polyclonal Antibody (Product # PA5-42408) and a 113 kDa band corresponding to SLC12A3 was observed in the cell lines tested along with an uncharacterized band (*) at ~18 kDa. Membrane enriched cell extracts (30 µg lysate) of HeLa (Lane 1), Hep G2 (Lane 2), SK-OV-3 (Lane 3), and HEK-293 (Lane 4) were electrophoresed using Novex® NuPAGE® 4-12 % Bis-Tris gel (Product # NP0321BOX). Resolved proteins were then transferred onto a nitrocellulose membrane (Product # IB23001) by iBlot® 2 Dry Blottin... View More
Product Details
PA5-42408
Product Specifications
Species Reactivity
Human
Host/Isotype
Rabbit
/ IgG
Class
Polyclonal
Type
Antibody
Immunogen
Synthetic peptide directed towards the middle region of human SLC12A3
Conjugate
Form
Liquid
Concentration
0.5 mg/mL
Purification
Affinity Chromatography
Storage buffer
PBS with 2% sucrose
Contains
0.09% sodium azide
Storage conditions
-20°C, Avoid Freeze/Thaw Cycles
Shipping conditions
Ambient (domestic); Wet ice (international)
RRID
AB_2608511
Product Specific Information
Peptide sequence: ALIVITLPIG RKGKCPSSLY MAWLETLSQD LRPPVILIRG NQENVLTFYC
Sequence homology: Cow: 100%; Dog: 100%; Guinea Pig: 100%; Horse: 100%; Human: 100%; Mouse: 93%; Rabbit: 100%; Rat: 100%; Zebrafish: 86%
Target Information
The SLC12A3 gene encodes the thiazide-sensitive sodium-chloride symporter (NCC), which is critical for salt balance and blood pressure regulation in humans. This symporter is predominantly expressed in the distal convoluted tubule of the kidney, where it facilitates the reabsorption of sodium and chloride ions from the renal filtrate back into the bloodstream. NCC activity plays a vital role in maintaining extracellular fluid volume and potassium homeostasis, impacting systemic blood pressure. Mutations in SLC12A3 can lead to Gitelman syndrome, a hereditary salt-wasting disorder characterized by hypokalemic metabolic alkalosis, low blood pressure, and hypomagnesemia. This gene is regulated by the renin-angiotensin system, which modulates NCC activity through phosphorylation mediated by WNK kinases, thereby affecting renal sodium handling and blood pressure modulation. Research highlights the therapeutic potential of targeting NCC with thiazide diuretics to treat hypertension, emphasizing its importance in both physiological and pathological contexts. The dynamic regulation of NCC by hormonal pathways underscores its significance in fluid and electrolyte balance, contributing to cardiovascular health.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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Bioinformatics
Protein Aliases: chemokine (C-C); CK-beta-10; CKb10; MCP-4; MGC17134; Na-Cl cotransporter; Na-Cl symporter; NaCl electroneutral thiazide-sensitive cotransporter; NCC; NCC-1; NCC1; SCYA13; SCYL1; solute carrier family 12 (sodium/chloride transporter), member 3; Solute carrier family 12 member 3; thiazide-sensitive Na-Cl cotransporter; Thiazide-sensitive sodium-chloride cotransporter
Gene Aliases: NCC; NCCT; SLC12A3; TSC
UniProt ID: (Human) P55017
Entrez Gene ID: (Human) 6559
Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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