Search Thermo Fisher Scientific
Disclaimer
Clicking the images or links will redirect you to a website hosted by BenchSci that provides third-party scientific content. Neither the content nor the BenchSci technology and processes for selection have been evaluated by us; we are providing them as-is and without warranty of any kind, including for use or application of the Thermo Fisher Scientific products presented.
FIGURE: 1 / 1
SLC19A1 Antibody (PA5-88778) in WB
Western blot analysis of SLC19A1 in extracts of various cell lines. Samples were incubated with SLC19A1 Polyclonal antibody (Product # PA5-88778) using a dilution of 1:3,000, followed by HRP Goat Anti-Rabbit IgG (H+L) at a dilution of 1:10,000. Lysates/proteins: 25 µg per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 90s.
Product Details
PA5-88778
Product Specifications
Species Reactivity
Human
Host/Isotype
Rabbit
/ IgG
Class
Polyclonal
Type
Antibody
Immunogen
Recombinant fusion protein containing a sequence corresponding to amino acids 452-591 of human SLC19A1 (NP_9192311)
Conjugate
Form
Liquid
Concentration
0.38 mg/mL
Purification
Affinity Chromatography
Storage buffer
PBS, pH 7.3, with 50% glycerol
Contains
0.01% thimerosal
Storage conditions
-20°C, Avoid Freeze/Thaw Cycles
Shipping conditions
Ambient (domestic); Wet ice (international)
RRID
AB_2805125
Product Specific Information
Immunogen sequence: LDGLRHCQRG HHPRQPPAQG LRSAAEEKAA QALSVQDKGL GGLQPAQSPP LSPEDSLGAV GPASLEQRQS DPYLAQAPAP QAAEFLSPVT TPSPCTLCSA QASGPEAADE TCPQLAVHPP GVSKLGLQCL PSDGVQNVNQ; Positive Samples: HeLa, U-87MG; Cellular Location: Membrane, Multi-pass membrane protein
Target Information
Transport of folate compounds into mammalian cells can occur via receptor-mediated or carrier-mediated mechanisms. A functional coordination between these 2 mechanisms has been proposed to be the method of folate uptake in certain cell types. Methotrexate (MTX) is an antifolate chemotherapeutic agent that is actively transported by the carrier-mediated uptake system. RFC1 plays a role in maintaining intracellular concentrations of folate.
⚠WARNING: This product can expose you to chemicals including mercury, which is known to the State of California to cause birth defects or other reproductive harm. For more information go to www.P65Warnings.ca.gov.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
References (0)
Have you cited this product in a publication?
Let us know so we can reference it here.
Bioinformatics
Protein Aliases: Cyclic dinucleotide:anion antiporter SLC19A1; Folate:anion antiporter SLC19A1; FOLT; hSLC19A1; IFC-1; Intestinal folate carrier 1; Placental folate transporter; Reduced folate carrier protein; Reduced folate transporter; Reduced folate transporter 1; RFC; RFT-1; solute carrier family 19 (folate transporter), member 1; Solute carrier family 19 member 1
Gene Aliases: CHMD; FLOT1; FOLT; IFC1; REFC; RFC1; SLC19A1
UniProt ID: (Human) P41440
Entrez Gene ID: (Human) 6573
Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
Learn more
We're here to help
Get expert recommendations for common problems or connect directly with an on staff expert for technical assistance related to applications, equipment and general product use.
Contact tech support