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SLC27A5 Antibody (PA5-101463) in ICC/IF
Immunocytochemistry analysis of SLC27A5 in HepG2 cells. The samples were fixed with PFA and permeabilized in 0.1% Triton X-100, then blocked in 10% serum for 45 minutes at 25°C. Samples were incubated with SLC27A5 polyclonal antibody (Product # PA5-101463) and mouse anti-beta tubulin antibody for 1 hour at 37°C. An AlexaFluor594 conjugated goat anti-rabbit IgG (Red) and an AlexaFluor488 conjugated goat anti-mouse IgG (Green) were used as the secondary antibodies. The nuclear counter stain is DAPI (blue).
Product Details
PA5-101463
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Human,
Mouse,
Rat
Host/Isotype
Rabbit
/ IgG
Class
Polyclonal
Type
Antibody
Immunogen
A synthesized peptide derived from human SLC27A5(Accession Q9Y2P5), corresponding to amino acid residues P486-V536.
Conjugate
Form
Liquid
Concentration
1 mg/mL
Purification
Affinity chromatography
Storage buffer
PBS, pH 7.4, with 50% glycerol
Contains
0.02% sodium azide
Storage conditions
-20°C
Shipping conditions
Ambient (domestic); Wet ice (international)
RRID
AB_2850898
Product Specific Information
Antibody detects endogenous levels of total SLC27A5.
Target Information
The protein encoded by this gene is an isozyme of very long-chain acyl-CoA synthetase (VLCS). It is capable of activating very long-chain fatty-acids containing 24- and 26-carbons. It is expressed in liver and associated with endoplasmic reticulum but not with peroxisomes. Its primary role is in fatty acid elongation or complex lipid synthesis rather than in degradation. This gene has a mouse ortholog.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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Bioinformatics
Protein Aliases: BA-CoA ligase; BACS; BAL; bile acid CoA ligase; Bile acid-CoA ligase; Bile acyl-CoA synthetase; Cholate--CoA ligase; FATP-5; fatty acid transport protein 5; Fatty-acid-coenzyme A ligase, very long-chain 3; FLJ22987; Long-chain fatty acid transport protein 5; Long-chain-fatty-acid--CoA ligase; solute carrier family 27 (fatty acid transporter), member 5; Solute carrier family 27 member 5; ve; Very long-chain acyl-CoA synthetase homolog 2; Very long-chain acyl-CoA synthetase-related protein; very long-chain acyl-Coenzyme A dehydrogenase synthase related; VLACS-related; VLACSR; VLCS-H2
Gene Aliases: ACSB; ACSVL6; BACS; BAL; FACVL3; FATP-5; FATP5; rBAL-1; SLC27A5; VLACSR; VLCS-H2; VLCSH2
UniProt ID: (Human) Q9Y2P5, (Mouse) Q4LDG0, (Rat) Q9ES38
Entrez Gene ID: (Human) 10998, (Mouse) 26459, (Rat) 79111
very long-chain fatty acid metabolic process
long-chain fatty acid metabolic process
lipid metabolic process
fatty acid metabolic process
triglyceride mobilization
bile acid biosynthetic process
metabolic process
bile acid metabolic process
fatty acid transport
plasma membrane long-chain fatty acid transport
ketone body biosynthetic process
bile acid and bile salt transport
Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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