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Invitrogen
SLC5A5 Monoclonal Antibody (FP5), APC
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FIGURE: 1 / 5
SLC5A5 Antibody (MA5-45492) in IHC (PFA)
Immunohistochemistry analysis using Thyroid. Fixation involved 10% formalin solution for 12-24 hours at RT. Samples were incubated with Sodium Iodide Symporter monoclonal antibody (Product # MA5-45492) at 1:1,000 for 1 hour at RT, followed by HRP/DAB Detection System: Biotinylated Goat Anti-Mouse, Streptavidin Peroxidase, DAB Chromogen (brown) for 30 minutes at RT. Counterstain used was Mayer Hematoxylin (p... View More
Product Details
MA5-45492
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Human,
Mouse,
Rat
Host/Isotype
Mouse
/ IgG1, kappa
Class
Monoclonal
Type
Antibody
Clone
FP5
Immunogen
Mannose binding protein hNIS fusion (AA468-643)
Conjugate
Excitation/Emission Max
651/660 nm
View spectra
Form
Liquid
Concentration
1 mg/mL
Purification
Protein G
Storage buffer
95.64mM phosphate/2.48mM MES, pH 7.4, with 0.5M EDTA
Contains
no preservative
Storage conditions
4°C
Shipping conditions
Ambient (domestic); Wet ice (international)
RRID
AB_2931946
Product Specific Information
1 µg/mL of MA5-45492 was sufficient for detection of hNIS in 20 µg of transfected COS-7 cell membrane lysate by ECL immunoblot analysis using Goat anti-mouse IgG:HRP as the secondary antibody.|Detects approximately 97kDa, non-glycosylated version at 68kDa. Other minor bands associated with hNIS at 160kDa, and degradation products at approximately 30 kDa, and approximately 15kDa.
Target Information
Human Sodium Iodide Symporter (hNIS) is responsible for iodide concentrating ability within thyroid follicular cells. It is a membrane bound glycoprotein with 13 membrane spanning domains and 14 extramembranous domains. It may represent an autoantigen in thyroid.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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Bioinformatics
Protein Aliases: Na(+)/I(-) cotransporter; Na(+)/I(-) symporter; Na(+)/I(-)-symporter; Na+/I- symporter; Natrium iodide transporter; Sodium-iodide symporter; Sodium/iodide cotransporter; solute carrier family 5 (sodium iodide symporter), member 5; solute carrier family 5 (sodium/iodide cotransporter), member 5; Solute carrier family 5 member 5; solute carrier family 5, member 5
Gene Aliases: NIS; SLC5A5; TDH1
UniProt ID: (Human) Q92911, (Rat) Q63008
Entrez Gene ID: (Human) 6528, (Rat) 114613, (Mouse) 114479
Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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