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SLC6A4 Antibody (711108) in ICC/IF
For immunofluorescence analysis, IMR-32 cells were fixed and permeabilized for detection of endogenous SERT using Anti- SERT Recombinant Rabbit Superclonal™ Antibody (Product # 711108, 2 µg/mL) and labeled with Goat anti-Rabbit IgG (Heavy Chain) Superclonal™ Secondary Antibody, Alexa Fluor® 488 conjugate (Product # A27034, 1:2000). Panel a) shows representative cells that were stained for detection and localization of SERT protein (green), Panel b) is stained for nuclei (blue) using SlowFade® Gold Antifade Mountant with DAPI (Product # S36... View More
Product Details
711108
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Human,
Rat
Host/Isotype
Rabbit
/ IgG
Expression System
Expi293
Class
Recombinant Superclonal
Type
Antibody
Clone
16HCLC
Immunogen
Peptides corresponding to Human SLC6A4 (aa 444-462, 16-29)
Conjugate
Form
Liquid
Concentration
0.5 mg/mL
Purification
Protein A
Storage buffer
PBS, pH 7.2
Contains
0.09% sodium azide
Storage conditions
Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.
Shipping conditions
Wet ice
RRID
AB_2633125
Product Specific Information
This antibody is predicted to react with Monkey, Horse and Cat
Recombinant rabbit Superclonal™ antibodies are unique offerings from Thermo Fisher Scientific. They are comprised of a selection of multiple different recombinant monoclonal antibodies, providing the best of both worlds - the sensitivity of polyclonal antibodies with the specificity of monoclonal antibodies - all delivered with the consistency only found in a recombinant antibody. While functionally the same as a polyclonal antibody - recognizing multiple epitope sites on the target and producing higher detection sensitivity for low abundance targets - a recombinant rabbit Superclonal™ antibody has a known mixture of light and heavy chains. The exact population can be produced in every lot, circumventing the biological variability typically associated with polyclonal antibody production. Note: Formerly called “Recombinant polyclonal antibody”, this product is now rebranded as “Recombinant Superclonal™ antibody”. The physical product and the performance remain unchanged.
Target Information
SERT is an integral membrane protein that transports the neurotransmitter serotonin from synaptic spaces into presynaptic neurons. The encoded protein terminates the action of serotonin and recycles it in a sodium-dependent manner. This protein is a target of psychomotor stimulants, such as amphetamines and cocaine, and is a member of the sodium:neurotransmitter symporter family. A repeat length polymorphism in the promoter of this gene has been shown to affect the rate of serotonin uptake and may play a role in sudden infant death syndrome, aggressive behavior in Alzheimer disease patients, and depression-susceptibility in people experiencing emotional trauma.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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Bioinformatics
Protein Aliases: 5 OCD1; 5-hydroxytryptamine (serotonin) transporter; 5HT transporter; 5HTT; HGNC:11050; Na+/Cl- dependent serotonin transporter; OTTHUMP00000163633; serotonin transporter; serotonin transporter 1; SERT; Sodium-dependent serotonin transporter; solute carrier family 6 (neurotransmitter transporter serotonin) member 4 (5-hydroxytryptamine (serotonin) transporter); Solute carrier family 6 (neurotransmitter transporter serotonin) member 4 (5-hydroxytryptamine (serotonin) transporter); solute carrier family 6 (neurotransmitter transporter), member 4; solute carrier family 6 (neurotransmitter transporter, serotonin), member 4; Solute carrier family 6 member 4; solute carrier family 6, member 4
Gene Aliases: 5-HTT; 5-HTTLPR; 5HTT; hSERT; HTT; OCD1; SERT; SERT1; SLC6A4
UniProt ID: (Human) P31645, (Rat) P31652
Entrez Gene ID: (Human) 6532, (Rat) 25553
serotonin:sodium symporter activity
protein binding
monoamine transmembrane transporter activity
serotonin transmembrane transporter activity
myosin binding
syntaxin-1 binding
Rab GTPase binding
cocaine binding
protein homodimerization activity
metal ion binding
nitric-oxide synthase binding
actin filament binding
response to hypoxia
serotonin transport
brain development
response to nutrient
memory
circadian rhythm
locomotory behavior
response to toxic substance
response to organic substance
positive regulation of gene expression
monoamine transport
thalamus development
negative regulation of cerebellar granule cell precursor proliferation
negative regulation of synaptic transmission, dopaminergic
response to estradiol
social behavior
vasoconstriction
response to drug
sperm ejaculation
negative regulation of neuron differentiation
positive regulation of cell cycle
negative regulation of organ growth
brain morphogenesis
protein oligomerization
protein homooligomerization
serotonin uptake
transmembrane transport
cellular response to retinoic acid
cellular response to organic substance
cellular response to cGMP
chemical synaptic transmission
Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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