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SMAD1 Polyclonal Antibody

SMAD1 Antibody in Immunocytochemistry (ICC/IF)

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SMAD1 Antibody (10429-1-AP) in ICC/IF

Immunofluorescent analysis of HepG2 cells using 10429-1-AP (SMAD1 antibody) at dilution of 1:25 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG (H+L).

SMAD1 Antibody in Immunoprecipitation (IP)

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Product Details

10429-1-AP

Applications

Tested Dilution

Publications

Western Blot (WB)

1:500-1:1,000

View 20 publications 20 publications

Immunocytochemistry (ICC/IF)

1:10-1:100

Immunoprecipitation (IP)

0.5-4.0 µg

Product Specifications

Species Reactivity

Human, Mouse

Published species

Mouse

Host/Isotype

Rabbit / IgG

Class

Polyclonal

Type

Antibody

Immunogen

SMAD1 Fusion Protein Ag0701 (19-229 aa encoded by BC001878)

View immunogen

Conjugate

Unconjugated Unconjugated Unconjugated

Form

Liquid

Concentration

0.42 mg/mL

Purification

Antigen affinity chromatography

Storage buffer

PBS, pH 7.3, with 50% glycerol

Contains

0.02% sodium azide

Storage conditions

-20°C

Shipping conditions

Wet ice (domestic); Dry ice (international)

Product Specific Information

Immunogen sequence: GWKQGDEEE KWAEKAVDAL VKKLKKKKGA MEELEKALSC PGQPSNCVTI PRSLDGRLQV SHRKGLPHVI YCRVWRWPDL QSHHELKPLE CCEFPFGSKQ KEVCINPYHY KRVESPVLPP VLVPRHSEYN PQHSLLAQFR NLGQNEPHMP LNATFPDSFQ QPNSHPFPHS PNSSYPNSPG SSSSTYPHSP TSSDPGSPFQ MPADTPPPAY LP (19-229 aa encoded by BC001878)

Target Information

The protein encoded by this gene belongs to the SMAD, a family of proteins similar to the gene products of the Drosophila gene 'mothers against decapentaplegic' (Mad) and the C. elegans gene Sma. SMAD proteins are signal transducers and transcriptional modulators that mediate multiple signaling pathways. This protein mediates the signals of the bone morphogenetic proteins (BMPs), which are involved in a range of biological activities including cell growth, apoptosis, morphogenesis, development and immune responses. In response to BMP ligands, this protein can be phosphorylated and activated by the BMP receptor kinase. The phosphorylated form of this protein forms a complex with SMAD4, which is important for its function in the transcription regulation. This protein is a target for SMAD-specific E3 ubiquitin ligases, such as SMURF1 and SMURF2, and undergoes ubiquitination and proteasome-mediated degradation. Alternatively spliced transcript variants encoding the same protein have been observed.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

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Bioinformatics

Protein Aliases: BSP-1; Dwarfin-A; Dwf-A; DWFC; hSMAD1; JV4-1; JV5-1; MAD homolog 1; MAD, mothers against decapentaplegic homolog 1; Mad-related protein 1; MADH5; mMad1; Mothers against decapentaplegic homolog 1; mothers against DPP homolog 1; Mothers-against-DPP-related 1; Mothers-against-DPP-related-1; OTTHUMP00000220456; SMAD; SMAD 1; SMAD family member 1; SMAD, mothers against DPP homolog 1; SMAD5; TGF-beta signaling protein 1; transforming growth factor-beta signaling protein 1; Transforming growth factor-beta-signaling protein 1

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Gene Aliases: AI528653; BSP-1; BSP1; JV4-1; JV41; MADH1; MADR1; Mlp1; MusMLP; SMAD1

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UniProt ID: (Human) Q15797, (Mouse) P70340

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Entrez Gene ID: (Human) 4086, (Mouse) 17125

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Function(s)

RNA polymerase II core promoter proximal region sequence-specific DNA binding RNA polymerase II core promoter sequence-specific DNA binding transcriptional activator activity, RNA polymerase II core promoter proximal region sequence-specific binding transcription factor activity, sequence-specific DNA binding receptor signaling protein activity protein binding protein kinase binding transforming growth factor beta receptor, pathway-specific cytoplasmic mediator activity identical protein binding protein homodimerization activity metal ion binding protein heterodimerization activity co-SMAD binding I-SMAD binding DNA binding

Process(es)

MAPK cascade ureteric bud development mesodermal cell fate commitment osteoblast fate commitment transcription from RNA polymerase II promoter inflammatory response signal transduction transforming growth factor beta receptor signaling pathway SMAD protein complex assembly gamete generation negative regulation of cell proliferation embryonic pattern specification positive regulation of gene expression BMP signaling pathway midbrain development hindbrain development primary miRNA processing homeostatic process positive regulation of osteoblast differentiation positive regulation of transcription from RNA polymerase II promoter cartilage development cardiac muscle cell proliferation bone development SMAD protein signal transduction positive regulation of cartilage development positive regulation of transcription from RNA polymerase II promoter involved in cellular response to chemical stimulus transcription, DNA-templated regulation of transcription, DNA-templated regulation of transcription from RNA polymerase II promoter protein phosphorylation response to drug positive regulation of cell differentiation cellular response to organic cyclic compound cellular response to BMP stimulus

Performance Guarantee

If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*

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SMAD1 Polyclonal Antibody

SMAD1 Antibody (10429-1-AP) in ICC/IF

Product Details

10429-1-AP

Western Blot (WB)

Immunocytochemistry (ICC/IF)

Immunoprecipitation (IP)

Species Reactivity

Published species

Host/Isotype

Class

Type

Immunogen

Conjugate

Form

Concentration

Purification

Storage buffer

Contains

Storage conditions

Shipping conditions

Product Specific Information

Target Information

Bioinformatics

Performance Guarantee

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