Invitrogen

SMAD1/SMAD5/SMAD9 Polyclonal Antibody

View all (6) SMAD1/SMAD5/SMAD9 antibodies

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Datasheet
Protocols
Questions & Answers
Datasheet
Protocols
Questions & Answers

Product Details

PA5-104523

Applications
Tested Dilution
Publications

Western Blot (WB)

1:500-1:2,000
-

Immunohistochemistry (Paraffin) (IHC (P))

1:50-1:200
-

Immunocytochemistry (ICC/IF)

1:100-1:500
-
Product Specifications

Species Reactivity

Human, Mouse, Rat

Host/Isotype

Rabbit / IgG

Class

Polyclonal

Type

Antibody

Immunogen

A synthesized peptide derived from human SMAD1(Accession Q15797), corresponding to amino acid residues I438-S465.
View immunogen

Conjugate

Unconjugated Unconjugated Unconjugated

Form

Liquid

Concentration

1 mg/mL

Purification

Affinity chromatography

Storage buffer

PBS, pH 7.4, with 50% glycerol

Contains

0.02% sodium azide

Storage conditions

-20°C

Shipping conditions

Ambient (domestic); Wet ice (international)

RRID

AB_2853824

Product Specific Information

Antibody detects endogenous levels of total Smad1/5/9.

Target Information

The protein encoded by SMAD1/SMAD5/SMAD8/SMAD9 belongs to the SMAD, a family of proteins similar to the gene products of the Drosophila gene 'mothers against decapentaplegic' (Mad) and the C. elegans gene Sma. SMAD proteins are signal transducers and transcriptional modulators that mediate multiple signaling pathways. This protein mediates the signals of the bone morphogenetic proteins (BMPs), which are involved in a range of biological activities including cell growth, apoptosis, morphogenesis, development and immune responses. In response to BMP ligands, this protein can be phosphorylated and activated by the BMP receptor kinase. The phosphorylated form of this protein forms a complex with SMAD4, which is important for its function in the transcription regulation. This protein is a target for SMAD-specific E3 ubiquitin ligases, such as SMURF1 and SMURF2, and undergoes ubiquitination and proteasome-mediated degradation. Alternatively spliced transcript variants encoding the same protein have been observed.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

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Bioinformatics

Protein Aliases: BSP-1; Dwarfin-A; Dwarfin-C; Dwf-A; Dwf-C; JV4-1; JV5-1; MAD (mothers against decapentaplegic, Drosophila) homolog 1; MAD homolog 1; MAD homolog 5; MAD homolog 9; MAD homolog1 (mothers against decapentaplegic, Drosophila); MAD, mothers against decapentaplegic homolog 1; MAD, mothers against decapentaplegic homolog 5; Mad-related protein 1; Madh6; mMad1; Mothers against decapentaplegic homolog 1; Mothers against decapentaplegic homolog 5; Mothers against decapentaplegic homolog 9; mothers against decapentaplegic, drosophila, homolog of, 5; Mothers against decapentaplegic, drosophila, homolog of, 9; mothers against DPP homolog 1; mothers against DPP homolog 5; mothers against DPP homolog 9; Mothers-against-DPP-related 1; mSmad5; SMA- and MAD-related protein 5; SMAD; SMAD 1; SMAD 5; SMAD 9; SMAD family member 1; SMAD family member 5; SMAD family member 9; SMAD, mothers against DPP homolog 1; SMAD, mothers against DPP homolog 5; SMAD, mothers against DPP homolog 9; Smad8; TGF-beta signaling protein 1; transforming growth factor-beta signaling protein 1; Transforming growth factor-beta-signaling protein 1

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Gene Aliases: 1110051M15Rik; AI451355; AI528653; BSP-1; BSP1; Dwf-C; DWFC; JV4-1; JV41; JV5-1; Mad1; MADH1; MADH5; MADH6; Madh8; MADH9; MADR1; Mlp1; Msmad5; MusMLP; PPH2; SMAD1; SMAD5; SMAD8; SMAD8/9; SMAD8A; SMAD8B; SMAD9

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UniProt ID: (Human) Q15797, (Human) Q99717, (Human) O15198, (Mouse) P70340, (Rat) P97588, (Mouse) P97454, (Rat) Q9R1V3, (Rat) O54835, (Mouse) Q9JIW5

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Entrez Gene ID: (Human) 4086, (Human) 4090, (Human) 4093, (Mouse) 17125, (Rat) 25671, (Mouse) 17129, (Rat) 59328, (Rat) 85435, (Mouse) 55994

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Function(s)
RNA polymerase II core promoter proximal region sequence-specific DNA binding RNA polymerase II core promoter sequence-specific DNA binding transcriptional activator activity, RNA polymerase II core promoter proximal region sequence-specific binding transcription factor activity, sequence-specific DNA binding receptor signaling protein activity protein binding protein kinase binding transforming growth factor beta receptor, pathway-specific cytoplasmic mediator activity identical protein binding protein homodimerization activity metal ion binding protein heterodimerization activity co-SMAD binding I-SMAD binding ubiquitin protein ligase binding DNA binding
Process(es)
ureteric bud development Mullerian duct regression osteoblast fate commitment transcription, DNA-templated protein phosphorylation signal transduction transforming growth factor beta receptor signaling pathway germ cell development embryonic pattern specification erythrocyte differentiation BMP signaling pathway positive regulation of osteoblast differentiation positive regulation of transcription, DNA-templated cartilage development cardiac muscle contraction bone development SMAD protein signal transduction cellular response to organic cyclic compound positive regulation of transcription from RNA polymerase II promoter involved in cellular response to chemical stimulus angiogenesis regulation of transcription, DNA-templated multicellular organism development cell differentiation positive regulation of transcription from RNA polymerase II promoter cellular response to BMP stimulus response to hypoxia midbrain development hindbrain development positive regulation of cell differentiation MAPK cascade mesodermal cell fate commitment transcription from RNA polymerase II promoter inflammatory response SMAD protein complex assembly gamete generation negative regulation of cell proliferation positive regulation of gene expression primary miRNA processing homeostatic process cardiac muscle cell proliferation positive regulation of cartilage development regulation of transcription from RNA polymerase II promoter response to drug kidney development response to organonitrogen compound negative regulation of muscle cell apoptotic process wound healing positive regulation of dendrite morphogenesis ossification regulation of ossification
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