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Product Details
PA5-78048
Product Specifications
Species Reactivity
Human
Host/Isotype
Rabbit
/ IgG
Class
Polyclonal
Type
Antibody
Immunogen
Carrier-protein conjugated synthetic peptide encompassing a sequence within the N-terminus region of Human SMAD1. The exact sequence is proprietary.
Conjugate
Form
Liquid
Concentration
0.85 mg/mL
Purification
Antigen affinity chromatography
Storage buffer
PBS, pH 7, with 20% glycerol
Contains
0.025% ProClin 300
Storage conditions
Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.
Shipping conditions
Ambient (domestic); Wet ice (international)
RRID
AB_2736629
Product Specific Information
Positive Control: SH-SY-5Y nuclear extract
Predicted Reactivity: Mouse (100%), Rat (100%), Zebrafish (100%), Xenopus laevis (100%), Pig (100%), Chicken (100%), Rhesus Monkey (100%), Bovine (100%)
Store product as a concentrated solution. Centrifuge briefly prior to opening the vial.
Target Information
The protein encoded by SMAD1/SMAD5/SMAD8/SMAD9 belongs to the SMAD, a family of proteins similar to the gene products of the Drosophila gene 'mothers against decapentaplegic' (Mad) and the C. elegans gene Sma. SMAD proteins are signal transducers and transcriptional modulators that mediate multiple signaling pathways. This protein mediates the signals of the bone morphogenetic proteins (BMPs), which are involved in a range of biological activities including cell growth, apoptosis, morphogenesis, development and immune responses. In response to BMP ligands, this protein can be phosphorylated and activated by the BMP receptor kinase. The phosphorylated form of this protein forms a complex with SMAD4, which is important for its function in the transcription regulation. This protein is a target for SMAD-specific E3 ubiquitin ligases, such as SMURF1 and SMURF2, and undergoes ubiquitination and proteasome-mediated degradation. Alternatively spliced transcript variants encoding the same protein have been observed.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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Bioinformatics
Protein Aliases: BSP-1; JV4-1; JV5-1; MAD homolog 1; MAD homolog 5; MAD homolog 9; MAD, mothers against decapentaplegic homolog 1; MAD, mothers against decapentaplegic homolog 5; Mad-related protein 1; Madh6; Mothers against decapentaplegic homolog 1; Mothers against decapentaplegic homolog 5; Mothers against decapentaplegic homolog 9; mothers against decapentaplegic, drosophila, homolog of, 5; Mothers against decapentaplegic, drosophila, homolog of, 9; mothers against DPP homolog 1; SMA- and MAD-related protein 5; SMAD; SMAD 1; SMAD 5; SMAD 9; SMAD family member 1; SMAD family member 5; SMAD family member 9; SMAD, mothers against DPP homolog 1; SMAD, mothers against DPP homolog 5; SMAD, mothers against DPP homolog 9; TGF-beta signaling protein 1; transforming growth factor-beta signaling protein 1; Transforming growth factor-beta-signaling protein 1
Gene Aliases: BSP-1; BSP1; DWFC; JV4-1; JV41; JV5-1; MADH1; MADH5; MADH6; MADH9; MADR1; PPH2; SMAD1; SMAD5; SMAD8; SMAD8/9; SMAD8A; SMAD8B; SMAD9
UniProt ID: (Human) Q15797, (Human) Q99717, (Human) O15198
Entrez Gene ID: (Human) 4086, (Human) 4090, (Human) 4093
RNA polymerase II core promoter proximal region sequence-specific DNA binding
RNA polymerase II core promoter sequence-specific DNA binding
transcriptional activator activity, RNA polymerase II core promoter proximal region sequence-specific binding
transcription factor activity, sequence-specific DNA binding
receptor signaling protein activity
protein binding
protein kinase binding
transforming growth factor beta receptor, pathway-specific cytoplasmic mediator activity
identical protein binding
protein homodimerization activity
metal ion binding
protein heterodimerization activity
co-SMAD binding
I-SMAD binding
ubiquitin protein ligase binding
DNA binding
ureteric bud development
Mullerian duct regression
osteoblast fate commitment
transcription, DNA-templated
protein phosphorylation
signal transduction
transforming growth factor beta receptor signaling pathway
germ cell development
embryonic pattern specification
erythrocyte differentiation
BMP signaling pathway
positive regulation of osteoblast differentiation
positive regulation of transcription, DNA-templated
cartilage development
cardiac muscle contraction
bone development
SMAD protein signal transduction
cellular response to organic cyclic compound
positive regulation of transcription from RNA polymerase II promoter involved in cellular response to chemical stimulus
MAPK cascade
mesodermal cell fate commitment
transcription from RNA polymerase II promoter
inflammatory response
SMAD protein complex assembly
gamete generation
negative regulation of cell proliferation
positive regulation of gene expression
midbrain development
hindbrain development
primary miRNA processing
homeostatic process
positive regulation of transcription from RNA polymerase II promoter
cardiac muscle cell proliferation
positive regulation of cartilage development
cellular response to BMP stimulus
Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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