Invitrogen

SMAD2 Polyclonal Antibody

Advanced Verification
2 References
View all (66) SMAD2 antibodies

Promotion

View available promotion(s)

Promo code: {{promo.promoCode}} {{promo.promoDescription}}. {{$productOrderCtrl.translations['antibody.pdp.commerceCard.promotion.learnmore']}}

Datasheet
Protocols
Questions & Answers
Datasheet
Protocols
Questions & Answers

Product Details

PA5-29237

Applications
Tested Dilution
Publications

Western Blot (WB)

1:500-1:3,000
View 1 publication 1 publication

Immunocytochemistry (ICC/IF)

1:100-1:1,000
-

Immunoprecipitation (IP)

1:500-1:1,000
-

ChIP assay (ChIP)

1.5 µg/10^6 cells
-

ChIP-sequencing (ChIP-Seq)

-
View 1 publication 1 publication
Product Specifications

Species Reactivity

Human, Mouse

Published species

Human

Host/Isotype

Rabbit / IgG

Class

Polyclonal

Type

Antibody

Immunogen

Recombinant protein encompassing a sequence within the center region of human SMAD2. The exact sequence is proprietary.

Conjugate

Unconjugated Unconjugated Unconjugated

Form

Liquid

Concentration

1 mg/mL

Purification

Antigen affinity chromatography

Storage buffer

0.1M tris glycine, pH 7, with 10% glycerol

Contains

0.01% thimerosal

Storage conditions

Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.

Shipping conditions

Ambient (domestic); Wet ice (international)

RRID

AB_2546713

Product Specific Information

Recommended positive controls: HeLa, SMAD2-transfected 293T.

Predicted reactivity: Mouse (99%), Rat (98%), Zebrafish (89%), Xenopus laevis (97%), Pig (85%), Chicken (99%), Rhesus Monkey (100%), Bovine (100%).

Store product as a concentrated solution. Centrifuge briefly prior to opening the vial.

Target Information

SMAD2 (MADH2, MAD2) regulates multiple cellular processes, such as cell proliferation, apoptosis, and differentiation. SMAD2 interacts with the TGF-beta receptors through its interaction with the SMAD anchor for receptor activation into the nucleus is a central event in TGF beta signaling. Phosphorylation of threonine 8 in the calmodulin-binding region of the MH1 domain by extracellular signal regulated kinase 1 (ERK1) enhances SMAD2 transcriptional activity, which is negatively regulated by calmodulin. In response to the TGF-beta signal, SMAD2 is phosphorylated by the TGF-beta receptors. The phosphorylation induces the dissociation of this protein with SARA and the association with the family member SMAD4. The association with SMAD4 is important for the translocation of this protein into the nucleus, where it binds to target promoters and forms a transcription repressor complex with other cofactors. SMAD2 can also be phosphorylated by activin type 1 receptor kinase, and mediates the signal from the activin. Alternatively spliced transcript variants encoding the SMAD2 protein have been observed.

⚠WARNING: This product can expose you to chemicals including mercury, which is known to the State of California to cause birth defects or other reproductive harm. For more information go to www.P65Warnings.ca.gov.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

Bioinformatics

Protein Aliases: hMAD-2; JV18-1; MAD; MAD homolog 2; Mad protein homolog; Mad-related protein 2; MGC22139; MGC34440; mMad2; mother against DPP homolog 2; Mothers against decapentaplegic homolog 2; mothers against DPP homolog 2; pSMAD2; Sma- and Mad-related protein 2; SMAD; SMAD 2; SMAD family member 2; SMAD, mothers against DPP homolog 2; Smad2Deltaexon3

View more View less

Gene Aliases: 7120426M23Rik; hMAD-2; hSMAD2; JV18; JV18-1; MADH2; MADR2; mMad2; Smad-2; SMAD2

View more View less

UniProt ID: (Human) Q15796, (Mouse) Q62432

View more View less

Entrez Gene ID: (Human) 4087, (Mouse) 17126

View more View less

Function(s)
RNA polymerase II core promoter proximal region sequence-specific DNA binding transcriptional activator activity, RNA polymerase II core promoter proximal region sequence-specific binding DNA binding chromatin binding double-stranded DNA binding transcription factor activity, sequence-specific DNA binding transforming growth factor beta receptor binding protein binding transcription factor binding phosphatase binding transforming growth factor beta receptor, pathway-specific cytoplasmic mediator activity ubiquitin protein ligase binding activating transcription factor binding type I transforming growth factor beta receptor binding enhancer binding protein homodimerization activity SMAD binding metal ion binding protein heterodimerization activity co-SMAD binding I-SMAD binding R-SMAD binding
Process(es)
negative regulation of transcription from RNA polymerase II promoter ureteric bud development in utero embryonic development endoderm formation mesoderm formation transcription from RNA polymerase II promoter transforming growth factor beta receptor signaling pathway common-partner SMAD protein phosphorylation SMAD protein complex assembly zygotic specification of dorsal/ventral axis gastrulation negative regulation of cell proliferation response to glucose post-embryonic development anterior/posterior pattern specification positive regulation of epithelial to mesenchymal transition regulation of transforming growth factor beta receptor signaling pathway signal transduction involved in regulation of gene expression insulin secretion lung development negative regulation of transforming growth factor beta receptor signaling pathway positive regulation of BMP signaling pathway pancreas development primary miRNA processing activin receptor signaling pathway somatic stem cell population maintenance organ growth intracellular signal transduction nodal signaling pathway cell fate commitment negative regulation of transcription, DNA-templated positive regulation of transcription, DNA-templated positive regulation of transcription from RNA polymerase II promoter paraxial mesoderm morphogenesis embryonic foregut morphogenesis embryonic cranial skeleton morphogenesis regulation of binding palate development pericardium development SMAD protein signal transduction response to cholesterol positive regulation of nodal signaling pathway involved in determination of lateral mesoderm left/right asymmetry transcription, DNA-templated regulation of transcription, DNA-templated protein phosphorylation pattern specification process endoderm development heart development embryonic pattern specification positive regulation of gene expression negative regulation of gene expression developmental growth
guarantee_icon

Performance Guarantee

If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*

Learn more
help_icon

We're here to help

Get expert recommendations for common problems or connect directly with an on staff expert for technical assistance related to applications, equipment and general product use.

Contact tech support