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Store vial at -20° C prior to opening. Aliquot contents and freeze at -20° C or below for extended storage. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use.
SMAD3 affinity-purified antibody is directed against human Smad3 protein. A BLAST analysis was used to suggest reactivity with this protein from human, mouse, rat, swine, and chicken sources based on 100% homology for the immunogen sequence. ELISA and western blot show equivalent reactivity against phosphorylated and non-phosphorylated human SMAD3.
SMAD3 belongs to the SMAD family of proteins. They are similar to the gene products of the Drosophila gene 'mothers against decapentaplegic' (Mad) and the C. elegans gene Sma. SMAD proteins are signal transducers and transcriptional modulators that mediate multiple signaling pathways. This protein functions as a transcriptional modulator activated by transforming growth factor-beta and is thought to play a role in the regulation of carcinogenesis.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
Protein Aliases: DKFZp586N0721; DKFZp686J10186; hMAD-3; hSMAD3; JV15-2; MAD (mothers against decapentaplegic, Drosophila) homolog 3; MAD homolog 3; MAD homolog 3a; mad homolog JV15-2; mad protein homolog; MAD, mothers against decapentaplegic homolog 3; MAD-3; mad3; MGC60396; mMad3; Mothers against decapentaplegic homolog 3; mothers against DPP homolog 3; SMA- and MAD-related protein 3; SMAD; SMAD 3; SMAD family member 3; SMAD, mothers against DPP homolog 3; TGF beta response effector Smad3; TGF-beta response effector Smad3; transcription factor
Gene Aliases: AU022421; BOS_10264; HSPC193; HsT17436; JV15-2; LDS1C; LDS3; mad3; MADH3; madh3-A; madh3a; Smad 3; SMAD3; wu:fa99e03; XELAEV_18018454mg; XenMLP; Xmad3; XSmad3
UniProt ID: (Human) P84022, (Pig) P84024, (Chicken) P84023, (Mouse) Q8BUN5, (Rat) P84025
Entrez Gene ID: (Human) 4088, (Pig) 397260, (Chicken) 395132, (Bovine) 515125, (Mouse) 17127, (Rat) 25631, (Xenopus) 378633, (Zebrafish) 58092
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