NeoBiotechnologies

SMAD4 (Pancreatic Adenocarcinoma Marker) Monoclonal Antibody (SMAD4/2440)

View all (69) SMAD4 antibodies

Promotion

View available promotion(s)

Promo code: {{promo.promoCode}} {{promo.promoDescription}}. {{$productOrderCtrl.translations['antibody.pdp.commerceCard.promotion.learnmore']}}

Datasheet
Protocols
Questions & Answers
Datasheet
Protocols
Questions & Answers

Product Details

4089-MSM1-P1ABX

Applications
Tested Dilution
Publications

Immunohistochemistry (Paraffin) (IHC (P))

Assay-dependent
-

Immunohistochemistry (PFA fixed) (IHC (PFA))

1-2 µg/mL
-

Peptide Array (Array)

Assay-dependent
-
Product Specifications

Species Reactivity

Human

Host/Isotype

Mouse / IgG2a, kappa

Class

Monoclonal

Type

Antibody

Clone

SMAD4/2440

Immunogen

Recombinant full-length human STAT6 protein
View immunogen

Conjugate

Unconjugated Unconjugated Unconjugated

Form

Liquid

Concentration

1 mg/mL

Purification

Protein A/G

Storage buffer

PBS, pH 7.4

Contains

no preservative

Storage conditions

-20°C, Avoid Freeze/Thaw Cycles

Shipping conditions

Wet ice

Target Information

Smad4 is encoded by the Smad4 gene that is located on chromosome 18 in humans. Smad4 is also known as Smad family member 4, Sma- and Mad-related protein 4 or Mothers against decapentaplegic (MAD) homolog 4. Smad4 contains N-terminal MH1 (MAD homology 1) and C-terminal MH2 (MAD homology 2) globular domains that are involved in DNA binding and protein interactions respectively. Binding of the TGF-beta superfamily of ligands that includes Transforming Growth Factor -beta (TGF-beta) and bone morphogenetic protein (BMP) to its cognate receptor allows phosphorylation of Smad 1, 2, 3, 5 and 8 (R-Smad, Receptor Smad). This signals for heterotrimerization with Smad4 (co-Smad, co-mediator Smad) and translocation of the complex to the nucleus. Inhibitory or antagonistic Smad (I-Smad) that includes Smad 6 and 7, interact with activated R-Smads and attenuate the signaling pathway. Smad4 acts as a tumor suppressor protein by transcriptionally regulating its target genes such as Cyclin D1 (downregulation) and collagen (upregulation) that inhibit cell proliferation. Dephosphorylation regulates nuclear export and nucleocytoplasmic dynamics of Smads.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

References (0)

Have you cited this product in a publication?
Let us know so we can reference it here.
Cite this product

Bioinformatics

Protein Aliases: Deleted in Pancreatic Carcinoma 4 (DPC4); deleted in pancreatic carcinoma locus 4; Deletion target in pancreatic carcinoma 4; hSMAD4; MAD homolog 4; MAD homolog 4 (MADH4); Mothers against decapentaplegic homolog 4; Mothers against decapentaplegic homolog 4 (MADH4); mothers against decapentaplegic, Drosophila, homolog of, 4; SMAD; SMAD 4; SMAD family member 4; SMAD, mothers against DPP homolog 4

View more View less

Gene Aliases: DPC4; JIP; MADH4; MYHRS; SMAD4

View more View less

UniProt ID: (Human) Q13485

View more View less

Entrez Gene ID: (Human) 4089

View more View less

Function(s)
RNA polymerase II core promoter proximal region sequence-specific DNA binding core promoter proximal region sequence-specific DNA binding transcription factor activity, protein binding transcription factor activity, RNA polymerase II transcription factor binding transcriptional activator activity, RNA polymerase II core promoter proximal region sequence-specific binding RNA polymerase II transcription factor binding DNA binding chromatin binding transcription factor activity, sequence-specific DNA binding protein binding collagen binding transforming growth factor beta receptor, common-partner cytoplasmic mediator activity identical protein binding protein homodimerization activity sequence-specific DNA binding transcription regulatory region DNA binding metal ion binding protein heterodimerization activity I-SMAD binding R-SMAD binding
Process(es)
negative regulation of transcription from RNA polymerase II promoter ovarian follicle development branching involved in ureteric bud morphogenesis response to hypoxia in utero embryonic development gastrulation with mouth forming second atrioventricular valve formation epithelial to mesenchymal transition involved in endocardial cushion formation left ventricular cardiac muscle tissue morphogenesis positive regulation of cell proliferation involved in heart valve morphogenesis brainstem development transcription from RNA polymerase II promoter cellular iron ion homeostasis transforming growth factor beta receptor signaling pathway SMAD protein complex assembly spermatogenesis single fertilization axon guidance endoderm development mesoderm development cell proliferation negative regulation of cell proliferation negative regulation of cardiac muscle hypertrophy positive regulation of epithelial to mesenchymal transition positive regulation of pathway-restricted SMAD protein phosphorylation neural crest cell differentiation regulation of transforming growth factor beta receptor signaling pathway negative regulation of cell growth BMP signaling pathway positive regulation of transforming growth factor beta receptor signaling pathway positive regulation of BMP signaling pathway somite rostral/caudal axis specification regulation of transforming growth factor beta2 production positive regulation of luteinizing hormone secretion somatic stem cell population maintenance intracellular signal transduction atrioventricular canal development endothelial cell activation embryonic digit morphogenesis negative regulation of transcription, DNA-templated positive regulation of transcription, DNA-templated positive regulation of transcription from RNA polymerase II promoter positive regulation of follicle-stimulating hormone secretion developmental growth neuron fate commitment sebaceous gland development formation of anatomical boundary regulation of binding positive regulation of histone H3-K4 methylation regulation of hair follicle development palate development uterus development positive regulation of SMAD protein import into nucleus SMAD protein signal transduction ventricular septum morphogenesis negative regulation of cell death endocardial cell differentiation female gonad morphogenesis interleukin-6-mediated signaling pathway negative regulation of ERK1 and ERK2 cascade response to transforming growth factor beta cellular response to BMP stimulus metanephric mesenchyme morphogenesis nephrogenic mesenchyme morphogenesis seminiferous tubule development positive regulation of transcription from RNA polymerase II promoter involved in cellular response to chemical stimulus positive regulation of histone H3-K9 acetylation
guarantee_icon

Performance Guarantee

If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*

Learn more
help_icon

We're here to help

Get expert recommendations for common problems or connect directly with an on staff expert for technical assistance related to applications, equipment and general product use.

Contact tech support