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Invitrogen
SMAD6 Polyclonal Antibody
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SMAD6 Antibody (PA5-25263) in WB
Western blot analysis of SMAD6 in Human lung whole cell lysate. Samples were incubated with SMAD6 polyclonal antibody (Product # PA5-25263) using a dilution of 1:500 followed by Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at a dilution of 1:10,000. Lysates/proteins: 20 µg per lane. Predicted band size: 53 kDa. Blocking/Dilution buffer: 5% NFDM/TBST.
Product Details
PA5-25263
Product Specifications
Species Reactivity
Human
Host/Isotype
Rabbit
/ IgG
Class
Polyclonal
Type
Antibody
Immunogen
KLH conjugated synthetic peptide between 358-386 amino acids from the central region of human SMAD6
Conjugate
Form
Liquid
Concentration
0.46 mg/mL
Purification
Protein A, Antigen affinity chromatography
Storage buffer
PBS, pH 7.4
Contains
0.09% sodium azide
Storage conditions
Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.
Shipping conditions
Ambient (domestic); Wet ice (international)
RRID
AB_2542763
Product Specific Information
This antibody is predicted to react with mouse based on sequence homology.
Target Information
SMAD6 (mothers against decapentaplegic homolog 6) is an antagonist of signaling by TGF-beta (transforming growth factor) type 1 receptor superfamily members; has been shown to inhibit selectively BMP (bone morphogenetic proteins) signaling by competing with the co-SMAD SMAD4 for receptor-activated SMAD1. SMAD6 is an inhibitory SMAD (I-SMAD) or antagonistic SMAD.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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Bioinformatics
Protein Aliases: MAD homolog 6; Mothers against decapentaplegic homolog 6; mothers against decapentaplegic, drosophila, homolog of, 6; SMAD; SMAD 6; SMAD family member 6; SMAD SMAD; SMAD, mothers against DPP homolog 6
Gene Aliases: AOVD2; HsT17432; MADH6; MADH7; SMAD6
UniProt ID: (Human) O43541
Entrez Gene ID: (Human) 4091
RNA polymerase II core promoter proximal region sequence-specific DNA binding
chromatin binding
transcription factor activity, sequence-specific DNA binding
protein binding
transforming growth factor beta receptor, inhibitory cytoplasmic mediator activity
ubiquitin protein ligase binding
type I transforming growth factor beta receptor binding
identical protein binding
transcription regulatory region DNA binding
metal ion binding
co-SMAD binding
I-SMAD binding
R-SMAD binding
type I activin receptor binding
ureteric bud development
outflow tract septum morphogenesis
mitral valve morphogenesis
pulmonary valve morphogenesis
ventricular septum development
transcription, DNA-templated
immune response
transforming growth factor beta receptor signaling pathway
zygotic specification of dorsal/ventral axis
negative regulation of cell proliferation
negative regulation of SMAD protein complex assembly
negative regulation of ossification
BMP signaling pathway
negative regulation of transforming growth factor beta receptor signaling pathway
negative regulation of BMP signaling pathway
cell-substrate adhesion
response to laminar fluid shear stress
aorta development
negative regulation of apoptotic process
response to estrogen
fat cell differentiation
negative regulation of pathway-restricted SMAD protein phosphorylation
coronary vasculature development
Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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