Invitrogen

SMAD7 Polyclonal Antibody

SMAD7 Antibody in Immunocytochemistry (ICC/IF)

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SMAD7 Antibody (42-0400) in ICC/IF

Immunofluorescence analysis of SMAD7 was done on 70% confluent log phase HeLa cells. The cells were fixed with 4% paraformaldehyde for 15 minutes, permeabilized with 0.25% Triton™ X-100 for 10 minutes, and blocked with 5% BSA for 1 hour at room temperature. The cells were labeled with SMAD7 Rabbit Polyclonal Antibody (Product # 42-0400) at 1 µg/mL in 1% BSA and incubated for 3 hours at room temperature and then labeled with Goat anti-Rabbit IgG (Heavy Chain) Superclonal™ Secondary Antibody, Alexa Fluor® 488 conjugate (Product # A27034) at ... View More

SMAD7 Antibody in Immunohistochemistry (Paraffin) (IHC (P))

Product Image Gallery

SMAD7 Antibody — Antibody specificity was demonstrated by siRNA mediated knockdown of target protein. HeLa cells were transfected with SMAD7 siRNA and decrease in signal intensity was observed in western blot application (Fig a) using Anti-SMAD7 Rabbit Polyclonal Antibody (Product # 42-0400). Densitometric analysis of this western blot is shown in histogram (Fig b). {KD}

Product Details

42-0400

Applications

Tested Dilution

Publications

Western Blot (WB)

1-2 µg/mL

View 12 publications 12 publications

Immunohistochemistry (IHC)

View 1 publication 1 publication

Immunohistochemistry (Paraffin) (IHC (P))

1:10-1:50

View 1 publication 1 publication

Immunocytochemistry (ICC/IF)

1 µg/mL

View 2 publications 2 publications

Product Specifications

Species Reactivity

Human, Mouse, Rat

Published species

Human, Mouse, Rat

Host/Isotype

Rabbit / IgG

Class

Polyclonal

Type

Antibody

Immunogen

Synthetic peptide derived from the C-terminal region of the human SMAD7

View immunogen

Conjugate

Unconjugated Unconjugated Unconjugated

Form

Liquid

Concentration

0.25 mg/mL

Purification

Antigen affinity chromatography

Storage buffer

PBS, pH 7.4

Contains

0.1% sodium azide

Storage conditions

-20°C

Shipping conditions

Ambient (domestic); Wet ice (international)

AB_2533512

Target Information

SMADs are members of the MAD-related family of molecules. MAD-related proteins are a family of intracellular proteins that are essential components in the signaling pathways of the serine/threonine kinase receptors of the transforming growth factor beta superfamily. SMADs can be divided into receptor-regulated SMADs (R-SMADs: SMAD1, SMAD2, SMAD5, SMAD8 and SMAD9), common-mediator SMAD (co-SMAD: SMAD4), and inhibitory SMADs (I-SMADs: SMAD6 and SMAD7). SMAD1, SMAD5, SMAD8 and SMAD9 have high degrees of homology and antibodies are available that recognize sequences common to all of them. SMAD8 and SMAD9 are typically used as alternate names for one another in the literature.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

Product References

Inhibition of TGF-β signaling, invasion, and growth of cutaneous squamous cell carcinoma by PLX8394.

Oncogene

Siljamäki E,Riihilä P,Suwal U,Nissinen L,Rappu P,Kallajoki M,Kähäri VM,Heino J

42-0400 was used in Western Blot to identify PLX8394 as a potential therapeutic compound for advanced human cSCC.

Fri Dec 01 00:00:00 UTC 2023

Inhibition of TGF-β signaling, invasion, and growth of cutaneous squamous cell carcinoma by PLX8394.

Oncogene

Siljamäki E,Riihilä P,Suwal U,Nissinen L,Rappu P,Kallajoki M,Kähäri VM,Heino J

42-0400 was used in Western Blot to identify PLX8394 as a potential therapeutic compound for advanced human cSCC.

Fri Dec 01 00:00:00 UTC 2023

The role of endogenous Smad7 in regulating macrophage phenotype following myocardial infarction.

FASEB journal : official publication of the Federation of American Societies for Experimental Biology

Li J,Li R,Tuleta I,Hernandez SC,Humeres C,Hanna A,Chen B,Frangogiannis NG

42-0400 was used in Immunocytochemistry, Immunohistochemistry (Paraffin), Western Blot to suggest a limited role for macrophage Smad7 in regulation of post-infarction inflammation and repair, and demonstrate that the anti-inflammatory effects of TGF-ß in macrophages are not restrained by endogenous Smad7 induction.

Fri Jul 01 00:00:00 UTC 2022

The acetyltransferase p300 is recruited in trans to multiple enhancer sites by lncSmad7.

Nucleic acids research

Maldotti M,Lauria A,Anselmi F,Molineris I,Tamburrini A,Meng G,Polignano IL,Scrivano MG,Campestre F,Simon LM,Rapelli S,Morandi E,Incarnato D,Oliviero S

42-0400 was used in Immunocytochemistry, Immunohistochemistry (Paraffin), Western Blot to find 122 lncRNAs that interacts directly with p300.

Mon Mar 21 00:00:00 UTC 2022

Cancer-associated fibroblasts downregulate type I interferon receptor to stimulate intratumoral stromagenesis.

Oncogene

Cho C,Mukherjee R,Peck AR,Sun Y,McBrearty N,Katlinski KV,Gui J,Govindaraju PK,Puré E,Rui H,Fuchs SY

42-0400 was used in Western Blot, Immunocytochemistry to investigate the importance of IFNAR1 inactivation in the non-immune tumor stroma.

Tue Sep 01 00:00:00 UTC 2020

Cancer-associated fibroblasts downregulate type I interferon receptor to stimulate intratumoral stromagenesis.

Oncogene

Cho C,Mukherjee R,Peck AR,Sun Y,McBrearty N,Katlinski KV,Gui J,Govindaraju PK,Puré E,Rui H,Fuchs SY

42-0400 was used in Western Blot, Immunocytochemistry to investigate the importance of IFNAR1 inactivation in the non-immune tumor stroma.

Tue Sep 01 00:00:00 UTC 2020

Cancer-associated fibroblasts downregulate type I interferon receptor to stimulate intratumoral stromagenesis.

Oncogene

Cho C,Mukherjee R,Peck AR,Sun Y,McBrearty N,Katlinski KV,Gui J,Govindaraju PK,Puré E,Rui H,Fuchs SY

42-0400 was used in Western Blot, Immunocytochemistry to investigate the importance of IFNAR1 inactivation in the non-immune tumor stroma.

Tue Sep 01 00:00:00 UTC 2020

HDAC inhibitors promote pancreatic stellate cell apoptosis and relieve pancreatic fibrosis by upregulating miR-15/16 in chronic pancreatitis.

Human cell

Ji T,Feng W,Zhang X,Zang K,Zhu X,Shang F

42-0400 was used in Western Blotting to find that miR-15 and miR-16 decreased significantly in chronic pancreatitis and HDAC inhibition could recover the levels of these two miRNAs.

Thu Oct 01 00:00:00 UTC 2020

HDAC inhibitors promote pancreatic stellate cell apoptosis and relieve pancreatic fibrosis by upregulating miR-15/16 in chronic pancreatitis.

Human cell

Ji T,Feng W,Zhang X,Zang K,Zhu X,Shang F

42-0400 was used in Western Blotting to find that miR-15 and miR-16 decreased significantly in chronic pancreatitis and HDAC inhibition could recover the levels of these two miRNAs.

Thu Oct 01 00:00:00 UTC 2020

Role of sialidase Neu3 and ganglioside GM3 in cardiac fibroblasts activation.

The Biochemical journal

Ghiroldi A,Piccoli M,Creo P,Cirillo F,Rota P,D'Imperio S,Ciconte G,Monasky MM,Micaglio E,Garatti A,Aureli M,Carsana EV,Menicanti L,Pappone C,Anastasia L

42-0400 was used in Western Blot to identify pro-fibrotic pathways in primary cultures of human cardiac fibroblasts as targets for cardiac fibrosis.

Fri Sep 18 00:00:00 UTC 2020

Effects of tacrolimus on the TGF‑β1/SMAD signaling pathway in paraquat‑exposed rat alveolar type II epithelial cells.

Molecular medicine reports

Ren Y,Jian X,Zhang Z,Ning Q,Kan B,Kong L

42-0400 was used in Immunocytochemistry-immunoflourescence to provide evidence that tacrolimus can reduce the levels of TGF‚β1, SMAD3 and CTGF, increase the level of SMAD7 in TGF‚β1 signaling pathway and protect the development of pulmonary fibrosis in paraquat exposed alveolar epithelial cells.

Sun Nov 01 00:00:00 UTC 2020

Effects of tacrolimus on the TGF‑β1/SMAD signaling pathway in paraquat‑exposed rat alveolar type II epithelial cells.

Molecular medicine reports

Ren Y,Jian X,Zhang Z,Ning Q,Kan B,Kong L

Sun Nov 01 00:00:00 UTC 2020

Deletion of Akt1 Promotes Kidney Fibrosis in a Murine Model of Unilateral Ureteral Obstruction.

BioMed research international

Kim IY,Lee MY,Park MW,Seong EY,Lee DW,Lee SB,Bae SS,Kim SS,Song SH

42-0400 was used in Western Blotting to show renal fibrosis can be promoted by Akt1 deletion by induction of the TGFβ1/STAT3 pathway.

Fri May 07 00:00:00 UTC 2021

Cryptotanshinone Protects Cartilage against Developing Osteoarthritis through the miR-106a-5p/GLIS3 Axis.

Molecular therapy. Nucleic acids

Ji Q,Qi D,Xu X,Xu Y,Goodman SB,Kang L,Song Q,Fan Z,Maloney WJ,Wang Y

42-0400 was used in Western Blotting to study the role of microRNAs in the therapeutic effect of cryptotanshinone in osteoarthritis.

Fri Jun 01 00:00:00 UTC 2018

SMAD7 regulates proinflammatory and prolabor mediators in amnion and myometrium.

Biology of reproduction

Lim R,Barker G,Lappas M

42-0400 was used in Immunohistochemistry on paraffin embedded tissues to study the effect of spontaneous human labour and prolabour mediators on SMAD7 expression in myometrium and fetal membranes.

Tue Aug 01 00:00:00 UTC 2017

SMAD7 regulates proinflammatory and prolabor mediators in amnion and myometrium.

Biology of reproduction

Lim R,Barker G,Lappas M

42-0400 was used in Immunohistochemistry on paraffin embedded tissues to study the effect of spontaneous human labour and prolabour mediators on SMAD7 expression in myometrium and fetal membranes.

Tue Aug 01 00:00:00 UTC 2017

Zika-Virus-Encoded NS2A Disrupts Mammalian Cortical Neurogenesis by Degrading Adherens Junction Proteins.

Cell stem cell

Yoon KJ,Song G,Qian X,Pan J,Xu D,Rho HS,Kim NS,Habela C,Zheng L,Jacob F,Zhang F,Lee EM,Huang WK,Ringeling FR,Vissers C,Li C,Yuan L,Kang K,Kim S,Yeo J,Cheng Y,Liu S,Wen Z,Qin CF,Wu Q,Christian KM,Tang H,Jin P,Xu Z,Qian J,Zhu H,Song H,Ming GL

42-0400 was used in Western Blot to study pathogenic mechanisms underlying Zika virus (ZIKV) infection in the developing mammalian brain.

Thu Sep 07 00:00:00 UTC 2017

Cucurbitacin I Attenuates Cardiomyocyte Hypertrophy via Inhibition of Connective Tissue Growth Factor (CCN2) and TGF- β/Smads Signalings.

PloS one

Jeong MH,Kim SJ,Kang H,Park KW,Park WJ,Yang SY,Yang DK

42-0400 was used in western blot to determine the functional role of cucurbitacin I using an in vitro model of cardiac hypertrophy.

Tue May 10 00:00:00 UTC 2016

Neuropilin-2 Is upregulated in lung cancer cells during TGF-β1-induced epithelial-mesenchymal transition.

Cancer research

Nasarre P,Gemmill RM,Potiron VA,Roche J,Lu X,Barón AE,Korch C,Garrett-Mayer E,Lagana A,Howe PH,Drabkin HA

42-0400 was used in western blot to study TGF-beta-driven lung cancer cell epithelial-to-mesenchymal transition and the role played by neuropilin-2.

Sun Dec 01 00:00:00 UTC 2013

Kruppel-like factor KLF10 targets transforming growth factor-beta1 to regulate CD4(+)CD25(-) T cells and T regulatory cells.

The Journal of biological chemistry

Cao Z,Wara AK,Icli B,Sun X,Packard RR,Esen F,Stapleton CJ,Subramaniam M,Kretschmer K,Apostolou I,von Boehmer H,Hansson GK,Spelsberg TC,Libby P,Feinberg MW

Fri Sep 11 00:00:00 UTC 2009

Bioinformatics

Protein Aliases: FLJ16482; hSMAD7; MAD (mothers against decapentaplegic, Drosophila) homolog 7; MAD homolog 7; MAD homolog 8; madh8; Mothers against decapentaplegic homolog 7; Mothers against decapentaplegic homolog 8; Mothers against decapentaplegic, drosophila, homolog of, 7; mothers against DPP homolog 7; mothers against DPP homolog 8; SMAD; SMAD 7; SMAD family member 7; SMAD, mothers against DPP homolog 7

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Gene Aliases: CRCS3; MADH7; MADH8; SMAD7

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UniProt ID: (Human) O15105, (Mouse) O35253, (Rat) O88406

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Entrez Gene ID: (Human) 4092, (Mouse) 17131, (Rat) 81516

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Function(s)

transcription factor activity, sequence-specific DNA binding protein binding collagen binding beta-catenin binding transforming growth factor beta receptor, inhibitory cytoplasmic mediator activity ubiquitin protein ligase binding type I transforming growth factor beta receptor binding transcription regulatory region DNA binding metal ion binding activin binding I-SMAD binding DNA binding

Process(es)

Performance Guarantee

If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*

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SMAD7 Polyclonal Antibody

SMAD7 Antibody (42-0400) in ICC/IF

Product Details

42-0400

Western Blot (WB)

Immunohistochemistry (IHC)

Immunohistochemistry (Paraffin) (IHC (P))

Immunocytochemistry (ICC/IF)

Species Reactivity

Published species

Host/Isotype

Class

Type

Immunogen

Conjugate

Form

Concentration

Purification

Storage buffer

Contains

Storage conditions

Shipping conditions

RRID

Target Information

Bioinformatics

Performance Guarantee

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