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          Clicking the images or links will redirect you to a website hosted by BenchSci that provides third-party scientific content. Neither the content nor the BenchSci technology and processes for selection have been evaluated by us; we are providing them as-is and without warranty of any kind, including for use or application of the Thermo Fisher Scientific products presented.

          • Primary Antibodies ›
          • SMARCC1 Antibodies

          Invitrogen

          SMARCC1 Recombinant Rabbit Monoclonal Antibody (1R7I6)

          Advanced Verification
          View all (15) SMARCC1 antibodies
          Datasheet
          Protocols
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          Datasheet
          Protocols
          Questions & Answers

          Cite SMARCC1 Recombinant Rabbit Monoclonal Antibody (1R7I6)

          • Antibody Testing Data (5)
          • Advanced Verification (1)
          SMARCC1 Antibody in Immunocytochemistry (ICC/IF)
          Group 53 Created with Sketch.
          SMARCC1 Antibody in Immunocytochemistry (ICC/IF)
          Group 53 Created with Sketch.

          FIGURE: 1 / 6

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          SMARCC1 Antibody (MA5-35475) in ICC/IF

          Immunofluorescence analysis of SMARCC1 was performed using 70% confluent log phase HeLa cells. The cells were fixed with 4% paraformaldehyde for 10 minutes, permeabilized with 0.1% Triton™ X-100 for 15 minutes, and blocked with 2% BSA for 1 hour at room temperature. The cells were labeled with SMARCC1 Recombinant Rabbit Monoclonal Antibody (ARC0948) (Product # MA5-35475) at 1:100 dilution in 0.1% BSA, incubated at 4 degree celsius overnight and then labeled with Donkey anti-Rabbit IgG (H+L) Highly Cross-Adsorbed Secondary Antibody, Alexa F... View More {{ $ctrl.currentElement.advancedVerification.fullName }} validation info. View more
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          SMARCC1 Antibody in Immunocytochemistry (ICC/IF)
          SMARCC1 Antibody in Immunocytochemistry (ICC/IF)
          SMARCC1 Antibody in Immunocytochemistry (ICC/IF)
          SMARCC1 Antibody in Western Blot (WB)
          SMARCC1 Antibody in ChIP Assay (ChIP)
          SMARCC1 Antibody

          Product Details

          MA5-35475

          Applications
          Tested Dilution
          Publications

          Western Blot (WB)

          1:500-1:2,000
          -

          Immunocytochemistry (ICC/IF)

          1:50-1:200
          -

          ELISA (ELISA)

          1 µg/mL
          -

          ChIP assay (ChIP)

          5 µg antibody for 10 µg-15 µg of Chromatin
          -
          Product Specifications

          Species Reactivity

          Human, Mouse

          Host/Isotype

          Rabbit / IgG

          Expression System

          HEK293 cells

          Class

          Recombinant Monoclonal

          Type

          Antibody

          Clone

          1R7I6

          Immunogen

          Recombinant fusion protein containing a sequence corresponding to amino acids 700-800 of human SMARCC1/BAF155 (Q92922)
          View immunogen

          Conjugate

          Unconjugated Unconjugated Unconjugated

          Form

          Liquid

          Concentration

          0.45 mg/mL

          Purification

          Affinity Chromatography

          Storage buffer

          PBS, pH 7.3, with 50% glycerol, 0.05% BSA

          Contains

          0.02% sodium azide

          Storage conditions

          -20°C, Avoid Freeze/Thaw Cycles

          Shipping conditions

          Ambient (domestic); Wet ice (international)

          RRID

          AB_2849376

          Product Specific Information

          Immunogen sequence: AFLASVVDPR VASAAAKAAL EEFSRVREEV PLELVEAHVK KVQEAARASG KVDPTYGLES SCIAGTGPDE PEKLEGAEEE KMEADPDGQQ PEKAENKVEN E

          Target Information

          The protein encoded by this gene is a member of the SWI/SNF family of proteins, whose members display helicase and ATPase activities and which are thought to regulate transcription of certain genes by altering the chromatin structure around those genes. The encoded protein is part of the large ATP-dependent chromatin remodeling complex SNF/SWI and contains a predicted leucine zipper motif typical of many transcription factors.

          For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

          References (0)

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          Cite this product

          Bioinformatics

          Protein Aliases: BAF155; BRG1-associated factor 155; chromatin remodeling complex BAF155 subunit; mammalian chromatin remodeling complex BRG1-associated factor 155; SWI/SNF complex 155 kDa subunit; SWI/SNF complex subunit SMARCC1; SWI/SNF related, matrix associated, actin dependent regulator of chromatin, subfamily c, member 1; SWI/SNF-related matrix-associated actin-dependent regulator of chromatin subfamily C member 1; SWI3-related protein

          View more View less

          Gene Aliases: AI115498; BAF155; CRACC1; Rsc8; SMARCC1; SRG3; SWI3

          View more View less

          UniProt ID: (Human) Q92922, (Mouse) P97496

          View more View less

          Entrez Gene ID: (Human) 6599, (Mouse) 20588

          View more View less

          Function(s)
          RNA polymerase II core promoter proximal region sequence-specific DNA binding RNA polymerase II distal enhancer sequence-specific DNA binding transcription coactivator activity protein binding nucleosomal DNA binding protein N-terminus binding DNA binding chromatin binding
          Process(es)
          DNA packaging nucleosome disassembly chromatin remodeling transcription, DNA-templated regulation of transcription, DNA-templated nervous system development insulin receptor signaling pathway organ morphogenesis chromatin modification negative regulation of proteasomal ubiquitin-dependent protein catabolic process ATP-dependent chromatin remodeling positive regulation of transcription, DNA-templated positive regulation of transcription from RNA polymerase II promoter regulation of transcription from RNA polymerase II promoter covalent chromatin modification prostate gland development
          It has to be done as per old AB suggested Products section.
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          Performance Guarantee

          If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*

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