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Invitrogen
SMC1 Recombinant Rabbit Monoclonal Antibody (SN20-27)
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FIGURE: 1 / 11
SMC1 Antibody (MA5-32341) in ICC/IF
Immunofluorescence analysis of SMC1A/SMC1B was performed using 70% confluent log phase U-2 OS cells. The cells were fixed with 4% paraformaldehyde for 10 minutes, permeabilized with 0.1% Triton™ X-100 for 15 minutes, and blocked with 2% BSA for 1 hour at room temperature. The cells were labeled with SMC1 Recombinant Rabbit Monoclonal Antibody (SN20-27) (Product # MA5-32341) at 5 µg/mL in 0.1% BSA, incubated at 4 degree celsius overnight and then labeled with Donkey anti-Rabbit IgG (H+L) Highly Cross-Adsorbed Secondary Antibody, Alexa Fluor... View More
Product Details
MA5-32341
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Human,
Mouse,
Rat
Host/Isotype
Rabbit
/ IgG
Expression System
HEK293 cells
Class
Recombinant Monoclonal
Type
Antibody
Clone
SN20-27
Immunogen
Synthetic peptide within Human SMC1A aa 1,184-1,233
Conjugate
Form
Liquid
Concentration
1 mg/mL
Purification
Protein A
Storage buffer
TBS, pH 7.4, with 40% Glycerol, 0.05% BSA
Contains
0.05% sodium azide
Storage conditions
Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.
Shipping conditions
Ambient (domestic); Wet ice (international)
RRID
AB_2809622
Product Specific Information
Recombinant rabbit monoclonal antibodies are produced using in vitro expression systems. The expression systems are developed by cloning in the specific antibody DNA sequences from immunoreactive rabbits. Then, individual clones are screened to select the best candidates for production. The advantages of using recombinant rabbit monoclonal antibodies include: better specificity and sensitivity, lot-to-lot consistency, animal origin-free formulations, and broader immunoreactivity to diverse targets due to larger rabbit immune repertoire.
Target Information
Structural Maintenance of Chromosomes (SMC) family proteins play critical roles in various nuclear events that require structural changes of chromosomes, including mitotic chromosome organization, DNA recombination and repair and global transcriptional repression. The chromo proteins are conserved in eukaryotes and lead to mitotic chromosome segregatior defects. This suggests a critical function of SMC family proteins in mitotic chromosome dynamics. SMC1 and SMC3 form a heterodimeric complex required for metaphase progression in mitotic cells. Specifically this SMC1/SMC3 complex is responsible for sister chromatid cohesion during metaphase. Mutations in the gene can lead to Cornelia de Lange syndrome 2.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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Bioinformatics
Protein Aliases: Chromosome segregation protein SmcB; DKFZp686L19178; DXhXs423e; MGC138332; mitosis-specific chromosome segregation protein like protein beta; RP6-29D12.1; Sb1.8; segregation of mitotic chromosomes 1; segregation of mitotic chromosomes b; segregation of mitotic chromosomes-like 1; SM1A; SMC; SMC (segregation of mitotic chromosomes 1)-like 1; SMC protein 1A; SMC protein 1B; SMC-1-alpha; SMC-1-beta; SMC-like 1; SMC-protein; SMC1 (structural maintenance of chromosomes 1, yeast)-like 1; SMC1 structural maintenance of chromosomes 1-like 2; SMC1alpha protein; structural maintenace of chromosomes 1B; structural maintenance of chromosomes 1 like 1; Structural maintenance of chromosomes protein 1A; Structural maintenance of chromosomes protein 1B
Gene Aliases: 5830426I24Rik; CDLS2; DXS423E; KIAA0178; mKIAA0178; SB1.8; SMC-1A; SMC-1B; SMC1; SMC1A; SMC1alpha; SMC1B; SMC1BETA; SMC1L1; SMC1L2; SMCB
UniProt ID: (Human) Q14683, (Human) Q8NDV3, (Rat) Q9Z1M9, (Mouse) Q9CU62, (Mouse) Q920F6
Entrez Gene ID: (Human) 8243, (Human) 27127, (Rat) 63996, (Mouse) 24061, (Rat) 300121, (Mouse) 140557
DNA repair
sister chromatid cohesion
mitotic sister chromatid cohesion
mitotic cell cycle checkpoint
meiotic nuclear division
response to radiation
stem cell population maintenance
negative regulation of DNA endoreduplication
signal transduction in response to DNA damage
cell division
mitotic sister chromatid segregation
mitotic spindle organization
protein sumoylation
cellular response to DNA damage stimulus
cell cycle
mitotic nuclear division
chromosome organization
meiotic cell cycle
Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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