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Invitrogen
SMC6 Polyclonal Antibody
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SMC6 Antibody (PA5-80042) in ICC/IF
Immunocytochemistry analysis of SMC6L1 using anti-SMC6L1 antibody (Product # PA5-80042) . SMC6L1 was detected in a section of A431 cells. Enzyme antigen retrieval was performed using IHC enzyme antigen retrieval reagent for 15 mins. The cells were blocked with 10% goat serum and then incubated with 2μg/mL rabbit anti-SMC6L1 antibody (Product # PA5-80042) overnight at 4°C. DyLight®488 Conjugated Goat Anti-Rabbit IgG was used as secondary antibody at 1:100 dilution and incubated for 30 minutes at 37°C. Visualize using a fluorescence microsco... View More
Product Details
PA5-80042
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Human,
Mouse,
Rat
Published species
Human
Host/Isotype
Rabbit
/ IgG
Class
Polyclonal
Type
Antibody
Immunogen
E. coli-derived human SMC6L1 recombinant protein (Position: D205-E443).
Conjugate
Form
Lyophilized
Concentration
500 µg/mL
Purification
Antigen affinity chromatography
Storage buffer
PBS with 4mg trehalose
Contains
0.05mg sodium azide
Storage conditions
-20°C
Shipping conditions
Ambient (domestic); Wet ice (international)
RRID
AB_2747157
Product Specific Information
Reconstitute with 0.2 mL of distilled water to yield a concentration of 500 µg/mL.
Positive Control - WB: human Hela whole cell, human HepG2 whole cell, human PANC-1 whole cell, human SK-OV-3 whole cell, human COLO-320 whole cell, rat testis tissue, mouse testis tissue. IHC: rat testis tissue, human testis tissue, mouse testis tissue. ICC/IF: A431 cell. Flow: A431 cell.
Target Information
Core component of the SMC5-SMC6 complex, a complex involved in DNA double-strand breaks by homologous recombination. The complex may promote sister chromatid homologous recombination by recruiting the SMC1-SMC3 cohesin complex to double-strand breaks. The complex is required for telomere maintenance via recombination in ALT (alternative lengthening of telomeres) cell lines and mediates sumoylation of shelterin complex (telosome) components which is proposed to lead to shelterin complex disassembly in ALT-associated PML bodies (APBs). Required for recruitment of telomeres to PML nuclear bodies. SMC5-SMC6 complex may prevent transcription of episomal DNA, such as circular viral DNA genome (PubMed:26983541). [UniProt]
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
Bioinformatics
Protein Aliases: mSMC6; SMC protein 6; SMC6 structural maintenance of chromosomes 6-like 1; Structural maintenance of chromosomes protein 6
Gene Aliases: 2810489L22Rik; 3830418C19Rik; AA990493; AU018782; AW742439; hSMC6; Kiaa4103; mKIAA4103; SMC-6; SMC6; SMC6L1
UniProt ID: (Human) Q96SB8, (Mouse) Q924W5
Entrez Gene ID: (Human) 79677, (Rat) 313961, (Mouse) 67241
Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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