Invitrogen

SMCR7 Polyclonal Antibody

1 Published Figure

View all (7) SMCR7 antibodies

Questions & Answers

Questions & Answers

Antibody Testing Data (5)
Published Figures (1)

SMCR7 Antibody in Immunocytochemistry (ICC/IF)

SMCR7 Antibody in Immunocytochemistry (ICC/IF)

FIGURE: 1 / 6

SMCR7 Antibody (PA5-99984) in ICC/IF

Immunofluorescent analysis of SMCR7 in HepG2. Samples were fixed with paraformaldehyde, permeabilized with 0.1% Triton X-100, blocked with 10% serum (45 min at 25°C) incubated with SMCR7 polyclonal antibody (Product # PA5-99984) using a dilution of 1:200 (1 hr, 37°C), and followed by goat anti-rabbit IgG Alexa Fluor 594 at a dilution of 1:600.

SMCR7 Antibody in Immunocytochemistry (ICC/IF)

SMCR7 Antibody in Immunohistochemistry (Paraffin) (IHC (P))

SMCR7 Antibody in Immunohistochemistry (Paraffin) (IHC (P))

SMCR7 Antibody in Western Blot (WB)

SMCR7 Antibody in Western Blot (WB)

SMCR7 Antibody in Western Blot, Immunocytochemistry, Proximity Ligation Assay (PLA) (WB, ICC/IF, PLA)

Product Image Gallery

SMCR7 Antibody in Immunocytochemistry (ICC/IF) — Immunofluorescent analysis of SMCR7 in HepG2. Samples were fixed with paraformaldehyde, permeabilized with 0.1% Triton X-100, blocked with 10% serum (45 min at 25°C) incubated with SMCR7 polyclonal antibody (Product # PA5-99984) using a dilution of 1:200 (1 hr, 37°C), and followed by goat anti-rabbit IgG Alexa Fluor 594 at a dilution of 1:600.

SMCR7 Antibody in Immunohistochemistry (Paraffin) (IHC (P)) — Immunohistochemistry analysis of SMCR7 in rat muscle tissue. The sample was formaldehyde fixed and a heat mediated antigen retrieval step in citrate buffer was performed. Samples were incubated with SMCR7 polyclonal antibody (Product # PA5-99984) using a dilution of 1:100 (4°C overnight) followed by HRP conjugated anti-Rabbit secondary antibody.

SMCR7 Antibody in Immunohistochemistry (Paraffin) (IHC (P)) — Immunohistochemistry analysis of SMCR7 in rat skin tissue. The sample was formaldehyde fixed and a heat mediated antigen retrieval step in citrate buffer was performed. Samples were incubated with SMCR7 polyclonal antibody (Product # PA5-99984) using a dilution of 1:100 (4°C overnight) followed by HRP conjugated anti-Rabbit secondary antibody.

SMCR7 Antibody in Western Blot (WB) — Western blot analysis of SMCR7 in various samples (Lane 1: 3T3 treated with blocking peptide, Lane 2: 3T3, Lane 3: COS-7, Lane 4: MCF7). Samples were incubated with SMCR7 polyclonal antibody (Product # PA5-99984).

SMCR7 Antibody in Western Blot (WB) — Western blot analysis of SMCR7 in HT-1080 cells. Samples were incubated with SMCR7 polyclonal antibody (Product # PA5-99984).

SMCR7 Antibody in Western Blot, Immunocytochemistry, Proximity Ligation Assay (PLA) (WB, ICC/IF, PLA) — Fig. 8 CLUH controls MiD49 and Mff protein levels through translational regulation in mammalian cells. a - d Immunofluorescence (IF) staining showing endogenous MiD49 and Mff protein levels in wild-type and CLUH KO cells, using anti-MiD49 and anti-Mff antibodies, respectively. e Quantification of IF signals of MiD49 and Mff, normalized to IF signals of a control mitochondrial protein TOM20 (images not shown) in the same cell, using Fiji/ImageJ. f , g Western blots showing Mff and MiD49 protein levels in response to CLUH overexpression or CLUH KO. VDAC was used as a loading control for mitochondrial mass, and actin was used as a housekeeping protein loading control. Western blot experiments were repeated independently 5 times, with representative images shown in ( f ) and statistical analysis shown in ( g ) (mean +- SEM, n = 5). h Quantitative PCR (qPCR) experiments showing Drp1 , Mff , and MiD49 mRNA levels in response to CLUH overexpression or CLUH KO. Total RNAs extracted from HeLa cells of the indicated genotypes were used as templates for the synthesis of cDNAs, which were then used as templates for qPCR. Experiments were repeated in triplicate (mean +- SEM, n = 3). i , j RNA Immunoprecipitation (RIP) experiments show that CLUH binds mRNAs of MiD49 and Mff , but not mRNAs of control genes TOM20 or TBP (encoding the nuclear TATA-binding protein). Experiments were repeated in triplicate (mean +- SEM, n = 3), and representative images are shown in ( j ). k - n ' Puro-PLA was

Product Details

PA5-99984

Applications

Tested Dilution

Publications

Western Blot (WB)

1:500-1:2,000

View 1 publication 1 publication

Immunohistochemistry (Paraffin) (IHC (P))

1:50-1:200

-

Immunocytochemistry (ICC/IF)

1:100-1:500

View 1 publication 1 publication

in situ PLA (PLA)

-

View 1 publication 1 publication

Product Specifications

Species Reactivity

Human, Mouse, Non-human primate, Rat

Published species

Fruit fly, Human

Host/Isotype

Rabbit / IgG

Class

Polyclonal

Type

Antibody

Immunogen

A synthesized peptide derived from human MIEF2(Accession Q96C03), corresponding to amino acid residues L325-R375.

Conjugate

Unconjugated Unconjugated Unconjugated

Form

Liquid

Concentration

1 mg/mL

Purification

Affinity chromatography

Storage buffer

PBS, pH 7.4, with 50% glycerol

Contains

0.02% sodium azide

Storage conditions

-20°C

Shipping conditions

Ambient (domestic); Wet ice (international)

AB_2818917

Product Specific Information

Antibody detects endogenous levels of total SMCR7/MID49.

Target Information

This gene encodes an outer mitochondrial membrane protein that functions in the regulation of mitochondrial morphology. It can directly recruit the fission mediator dynamin-related protein 1 (Drp1) to the mitochondrial surface. The gene is located within the Smith-Magenis syndrome region on chromosome 17. Alternative splicing results in multiple transcript variants encoding different isoforms.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

Product References

Clueless/CLUH regulates mitochondrial fission by promoting recruitment of Drp1 to mitochondria.

Nature communications

Yang H,Sibilla C,Liu R,Yun J,Hay BA,Blackstone C,Chan DC,Harvey RJ,Guo M

PA5-99984 was used in Western Blot, Immunocytochemistry, Proximity Ligation Assay (PLA) to identify a crucial role of Clueless and CLUH in controlling mitochondrial fission through regulation of Drp1.

Thu Mar 24 00:00:00 UTC 2022

Clueless/CLUH regulates mitochondrial fission by promoting recruitment of Drp1 to mitochondria.

Nature communications

Yang H,Sibilla C,Liu R,Yun J,Hay BA,Blackstone C,Chan DC,Harvey RJ,Guo M

PA5-99984 was used in Western Blot, Immunocytochemistry, Proximity Ligation Assay (PLA) to identify a crucial role of Clueless and CLUH in controlling mitochondrial fission through regulation of Drp1.

Thu Mar 24 00:00:00 UTC 2022

Clueless/CLUH regulates mitochondrial fission by promoting recruitment of Drp1 to mitochondria.

Nature communications

Yang H,Sibilla C,Liu R,Yun J,Hay BA,Blackstone C,Chan DC,Harvey RJ,Guo M

PA5-99984 was used in Western Blot, Immunocytochemistry, Proximity Ligation Assay (PLA) to identify a crucial role of Clueless and CLUH in controlling mitochondrial fission through regulation of Drp1.

Thu Mar 24 00:00:00 UTC 2022

Clueless/CLUH regulates mitochondrial fission by promoting recruitment of Drp1 to mitochondria.

Nature communications

Yang H,Sibilla C,Liu R,Yun J,Hay BA,Blackstone C,Chan DC,Harvey RJ,Guo M

PA5-99984 was used in Western Blot, Immunocytochemistry, Proximity Ligation Assay (PLA) to identify a crucial role of Clueless and CLUH in controlling mitochondrial fission through regulation of Drp1.

Thu Mar 24 00:00:00 UTC 2022

Clueless/CLUH regulates mitochondrial fission by promoting recruitment of Drp1 to mitochondria.

Nature communications

Yang H,Sibilla C,Liu R,Yun J,Hay BA,Blackstone C,Chan DC,Harvey RJ,Guo M

PA5-99984 was used in Western Blot, Immunocytochemistry, Proximity Ligation Assay (PLA) to identify a crucial role of Clueless and CLUH in controlling mitochondrial fission through regulation of Drp1.

Thu Mar 24 00:00:00 UTC 2022

Bioinformatics

Protein Aliases: mitochondrial dynamic protein MID49; mitochondrial dynamic protein of 49 kDa; mitochondrial dynamic protein of 49 kDa homolog; Mitochondrial dynamics protein MID49; Mitochondrial dynamics protein of 49 kDa; Mitochondrial dynamics protein of 49 kDa homolog; Mitochondrial elongation factor 2; Smith-Magenis syndrome chromosomal region candidate gene 7 protein; Smith-Magenis syndrome chromosomal region candidate gene 7 protein homolog; Smith-Magenis syndrome chromosome region, candidate 7; Smith-Magenis syndrome chromosome region, candidate 7 homolog

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Gene Aliases: AI482195; Gm11; MID49; MIEF2; RGD1560728; SMCR7

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UniProt ID: (Human) Q96C03, (Mouse) Q5NCS9

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Entrez Gene ID: (Human) 125170, (Mouse) 237781, (Rat) 497916

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protein binding molecular_function

mitochondrion organization positive regulation of protein homooligomerization positive regulation of mitochondrial fission mitochondrial fusion positive regulation of protein targeting to membrane

Performance Guarantee

If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*

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