Proteintech

SMN Polyclonal Antibody

View all (11) SMN1/SMN2 antibodies

Questions & Answers

Questions & Answers

Antibody Testing Data (28)

SMN Antibody in Immunocytochemistry (ICC/IF)

SMN Antibody in Immunocytochemistry (ICC/IF)

FIGURE: 1 / 28

SMN Antibody (11708-1-AP) in ICC/IF

Immunofluorescent analysis of (4% PFA) fixed HepG2 cells using SMN antibody (Product # 11708-1-AP) at dilution of 1:800 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L), CL594-Phalloidin (red).

SMN Antibody in Immunocytochemistry (ICC/IF)

SMN Antibody in Immunocytochemistry (ICC/IF)

SMN Antibody in Immunocytochemistry (ICC/IF)

SMN Antibody in Immunohistochemistry (Paraffin) (IHC (P))

SMN Antibody in Immunohistochemistry (Paraffin) (IHC (P))

SMN Antibody in Immunohistochemistry (Paraffin) (IHC (P))

SMN Antibody in Immunohistochemistry (Paraffin) (IHC (P))

SMN Antibody in Immunohistochemistry (Paraffin) (IHC (P))

SMN Antibody in Immunohistochemistry (Paraffin) (IHC (P))

SMN Antibody in Immunohistochemistry (Paraffin) (IHC (P))

SMN Antibody in Immunohistochemistry (Paraffin) (IHC (P))

SMN Antibody in Immunohistochemistry (Paraffin) (IHC (P))

SMN Antibody in Immunohistochemistry (Paraffin) (IHC (P))

SMN Antibody in Immunohistochemistry (PFA fixed) (IHC (PFA))

SMN Antibody in Western Blot (WB)

SMN Antibody in Western Blot (WB)

SMN Antibody in Western Blot (WB)

SMN Antibody in Western Blot (WB)

SMN Antibody in Western Blot (WB)

SMN Antibody in Western Blot (WB)

SMN Antibody in Western Blot (WB)

SMN Antibody in Western Blot (WB)

SMN Antibody in Western Blot (WB)

SMN Antibody in Western Blot (WB)

SMN Antibody in Western Blot (WB)

SMN Antibody in Western Blot (WB)

SMN Antibody in Immunoprecipitation (IP)

SMN Antibody in Immunoprecipitation (IP)

Product Image Gallery

SMN Antibody in Immunocytochemistry (ICC/IF) — Immunofluorescent analysis of (4% PFA) fixed HepG2 cells using SMN antibody (Product # 11708-1-AP) at dilution of 1:800 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L), CL594-Phalloidin (red).

SMN Antibody in Immunocytochemistry (ICC/IF) — Immunofluorescent analysis of HepG2 cells, using SMN2 antibody 11708-1-AP at 1:25 dilution and Rhodamine-labeled goat anti-rabbit IgG (red).

SMN Antibody in Immunocytochemistry (ICC/IF) — Immunofluorescent analysis of Hela cells, using SMN2 antibody 11708-1-AP at 1:25 dilution and Rhodamine-labeled goat anti-rabbit IGG (red).

SMN Antibody in Immunohistochemistry (Paraffin) (IHC (P)) — Immunohistochemistry of paraffin-embedded human placenta using 11708-1-AP (SMN antibody) at dilution of 1:100 (under 40x lens).

SMN Antibody in Immunohistochemistry (Paraffin) (IHC (P)) — Immunohistochemistry of paraffin-embedded human spleen using 11708-1-AP (SMN antibody) at dilution of 1:100 (under 40x lens).

SMN Antibody in Immunohistochemistry (Paraffin) (IHC (P)) — Immunohistochemistry of paraffin-embedded human testis using 11708-1-AP (SMN antibody) at dilution of 1:100 (under 40x lens).

SMN Antibody in Immunohistochemistry (Paraffin) (IHC (P)) — Immunohistochemistry of paraffin-embedded human kidney using 11708-1-AP (SMN antibody) at dilution of 1:100 (under 40x lens).

SMN Antibody in Immunohistochemistry (Paraffin) (IHC (P)) — Immunohistochemistry of paraffin-embedded human kidney using 11708-1-AP (SMN antibody) at dilution of 1:100 (under 10x lens).

SMN Antibody in Immunohistochemistry (Paraffin) (IHC (P)) — Immunohistochemistry of paraffin-embedded human ovary using 11708-1-AP (SMN antibody) at dilution of 1:100 (under 40x lens).

SMN Antibody in Immunohistochemistry (Paraffin) (IHC (P)) — Immunohistochemistry of paraffin-embedded human brain using 11708-1-AP (SMN antibody) at dilution of 1:100 (under 40x lens).

SMN Antibody in Immunohistochemistry (Paraffin) (IHC (P)) — Immunohistochemistry of paraffin-embedded human heart using 11708-1-AP (SMN antibody) at dilution of 1:50 (under 40x lens).

SMN Antibody in Immunohistochemistry (Paraffin) (IHC (P)) — Immunohistochemistry of paraffin-embedded human lung using 11708-1-AP (SMN antibody) at dilution of 1:100 (under 40x lens).

SMN Antibody in Immunohistochemistry (Paraffin) (IHC (P)) — Immunohistochemistry of paraffin-embedded human skin using 11708-1-AP (SMN antibody) at dilution of 1:100 (under 40x lens).

SMN Antibody in Immunohistochemistry (PFA fixed) (IHC (PFA)) — Immunofluorescent analysis of (4% PFA) fixed mouse brain tissue using 11708-1-AP (SMN antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG (H+L).

SMN Antibody in Western Blot (WB) — Various lysates were subjected to SDS PAGE followed by western blot with (Product # 11708-1-AP) SMN antibody at dilution of 1:3,000 incubated at room temperature for 1.5 hours.

SMN Antibody in Western Blot (WB) — Mouse testis tissue were subjected to SDS PAGE followed by western blot with 11708-1-AP (SMN antibody) at dilution of 1:1500 incubated at room temperature for 1.5 hours.

SMN Antibody in Western Blot (WB) — HEK-293 cells were subjected to SDS PAGE followed by western blot with 11708-1-AP (SMN antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

SMN Antibody in Western Blot (WB) — HEK-293 cells were subjected to SDS PAGE followed by western blot with 11708-1-AP (SMN antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

SMN Antibody in Western Blot (WB) — Jurkat cells were subjected to SDS PAGE followed by western blot with 11708-1-AP (SMN antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

SMN Antibody in Western Blot (WB) — K-562 cells were subjected to SDS PAGE followed by western blot with 11708-1-AP (SMN antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

SMN Antibody in Western Blot (WB) — HepG2 cells were subjected to SDS PAGE followed by western blot with 11708-1-AP (SMN antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

SMN Antibody in Western Blot (WB) — Jurkat cells were subjected to SDS PAGE followed by western blot with 11708-1-AP (SMN antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

SMN Antibody in Western Blot (WB) — HepG2 cells were subjected to SDS PAGE followed by western blot with 11708-1-AP (SMN antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

SMN Antibody in Western Blot (WB) — HeLa cells were subjected to SDS PAGE followed by western blot with 11708-1-AP (SMN antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

SMN Antibody in Western Blot (WB) — WB result of SMN antibody (Product # 11708-1-AP) 1:2,000, incubated at room temperature for 1.5 hours with sh-Control and sh-SMN transfected HeLa cells.

SMN Antibody in Western Blot (WB) — A549 cells (shcontrol and shRNA of SMN) were subjected to SDS PAGE followed by western blot with 11708-1-AP (SMN antibody) at dilution of 1:1000.

SMN Antibody in Immunoprecipitation (IP) — IP Result of anti-SMN (Product # 11708-1-AP), 3 µg; Detection (Product # 11708-1-AP) 1:1,000 with HEK-293 cells lysate 1000 µg.

SMN Antibody in Immunoprecipitation (IP) — IP result of anti-SMN2 (11708-1-AP for IP and Detection).

Product Details

11708-1-AP

Applications

Tested Dilution

Publications

Western Blot (WB)

1:500-1:2,000

View 3 publications 3 publications

Immunohistochemistry (Paraffin) (IHC (P))

1:50-1:200

-

Immunohistochemistry (PFA fixed) (IHC (PFA))

1:10-1:100

-

Immunocytochemistry (ICC/IF)

1:10-1:100

View 1 publication 1 publication

ELISA (ELISA)

-

View 4 publications 4 publications

Immunoprecipitation (IP)

0.5-4.0 µg

View 1 publication 1 publication

Miscellaneous PubMed (MISC)

-

View 4 publications 4 publications

Not applicable (N/A)

-

View 4 publications 4 publications

Product Specifications

Species Reactivity

Human, Mouse, Rat

Published species

Human, Mouse, Rat

Host/Isotype

Rabbit / IgG

Class

Polyclonal

Type

Antibody

Immunogen

SMN Fusion Protein Ag2260 (1-282 aa encoded by BC000908)

Conjugate

Unconjugated Unconjugated Unconjugated

Form

Liquid

Concentration

0.33 mg/mL

Purification

Antigen affinity chromatography

Storage buffer

PBS, pH 7.3, with 50% glycerol

Contains

0.02% sodium azide

Storage conditions

-20°C

Shipping conditions

Wet ice (domestic); Dry ice (international)

Product Specific Information

This antibody, 11708-1-AP, raised against the recombinant full-length human SMN2 protein, recognizes all isoforms of SMN protein.

Immunogen sequence: MAMSSGGSG GGVPEQEDSV LFRRGTGQSD DSDIWDDTAL IKAYDKAVAS FKHALKNGDI CETSGKPKTT PKRKPAKKNK SQKKNTAASL QQWKVGDKCS AIWSEDGCIY PATIASIDFK RETCVVVYTG YGNREEQNLS DLLSPICEVA NNIEQNAQEN ENESQVSTDE SENSRSPGNK SDNIKPKSAP WNSFLPPPPP MPGPRLGPGK PGLKFNGPPP PPPPPPPHLL SCWLPPFPSG PPIIPPPPPI CPDSLDDADA LGSMLISWYM SGYHTGYYME MLA (1-282 aa encoded by BC000908)

Target Information

The SMN1 gene is part of a 500 kb inverted duplication on chromosome 5q13. This duplicated region contains at least four genes and repetitive elements which make it prone to rearrangements and deletions. The repetitiveness and complexity of the sequence have also caused difficulty in determining the organization of this genomic region. The telomeric and centromeric copies of this gene are nearly identical and encode the same protein - survival motor neuron protein. The SMN complex plays a catalyst role in the assemble of small nuclear ribonucleoproteins, the building blocks of the spliceosome. Mutations in the SMN1 gene are known to cause spinal muscular atrophy 1/2.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

Product References

Hyper-SUMOylation of SMN induced by SENP2 deficiency decreases its stability and leads to spinal muscular atrophy-like pathology.

Journal of molecular medicine (Berlin, Germany)

Zhang Y,Chen X,Wang Q,Du C,Lu W,Yuan H,Zhang Z,Li D,Ling X,Ren X,Zhao Y,Su Q,Xing Z,Qin Y,Yang X,Shen Y,Wu H,Qi Y

Wed Dec 01 00:00:00 UTC 2021

Hyper-SUMOylation of SMN induced by SENP2 deficiency decreases its stability and leads to spinal muscular atrophy-like pathology.

Journal of molecular medicine (Berlin, Germany)

Zhang Y,Chen X,Wang Q,Du C,Lu W,Yuan H,Zhang Z,Li D,Ling X,Ren X,Zhao Y,Su Q,Xing Z,Qin Y,Yang X,Shen Y,Wu H,Qi Y

Wed Dec 01 00:00:00 UTC 2021

Hyper-SUMOylation of SMN induced by SENP2 deficiency decreases its stability and leads to spinal muscular atrophy-like pathology.

Journal of molecular medicine (Berlin, Germany)

Zhang Y,Chen X,Wang Q,Du C,Lu W,Yuan H,Zhang Z,Li D,Ling X,Ren X,Zhao Y,Su Q,Xing Z,Qin Y,Yang X,Shen Y,Wu H,Qi Y

Wed Dec 01 00:00:00 UTC 2021

Hyper-SUMOylation of SMN induced by SENP2 deficiency decreases its stability and leads to spinal muscular atrophy-like pathology.

Journal of molecular medicine (Berlin, Germany)

Zhang Y,Chen X,Wang Q,Du C,Lu W,Yuan H,Zhang Z,Li D,Ling X,Ren X,Zhao Y,Su Q,Xing Z,Qin Y,Yang X,Shen Y,Wu H,Qi Y

Wed Dec 01 00:00:00 UTC 2021

Hyper-SUMOylation of SMN induced by SENP2 deficiency decreases its stability and leads to spinal muscular atrophy-like pathology.

Journal of molecular medicine (Berlin, Germany)

Zhang Y,Chen X,Wang Q,Du C,Lu W,Yuan H,Zhang Z,Li D,Ling X,Ren X,Zhao Y,Su Q,Xing Z,Qin Y,Yang X,Shen Y,Wu H,Qi Y

Wed Dec 01 00:00:00 UTC 2021

Hyper-SUMOylation of SMN induced by SENP2 deficiency decreases its stability and leads to spinal muscular atrophy-like pathology.

Journal of molecular medicine (Berlin, Germany)

Zhang Y,Chen X,Wang Q,Du C,Lu W,Yuan H,Zhang Z,Li D,Ling X,Ren X,Zhao Y,Su Q,Xing Z,Qin Y,Yang X,Shen Y,Wu H,Qi Y

Wed Dec 01 00:00:00 UTC 2021

Dual SMN inducing therapies can rescue survival and motor unit function in symptomatic Δ7SMA mice.

Neurobiology of disease

Kray KM,McGovern VL,Chugh D,Arnold WD,Burghes AHM

Mon Nov 01 00:00:00 UTC 2021

Intragenic complementation of amino and carboxy terminal SMN missense mutations can rescue Smn null mice.

Human molecular genetics

McGovern VL,Kray KM,Arnold WD,Duque SI,Iyer CC,Massoni-Laporte A,Workman E,Patel A,Battle DJ,Burghes AHM

Sun Nov 01 00:00:00 UTC 2020

Mild SMN missense alleles are only functional in the presence of SMN2 in mammals.

Human molecular genetics

Iyer CC,Corlett KM,Massoni-Laporte A,Duque SI,Madabusi N,Tisdale S,McGovern VL,Le TT,Zaworski PG,Arnold WD,Pellizzoni L,Burghes AHM

Mon Oct 01 00:00:00 UTC 2018

Mild SMN missense alleles are only functional in the presence of SMN2 in mammals.

Human molecular genetics

Iyer CC,Corlett KM,Massoni-Laporte A,Duque SI,Madabusi N,Tisdale S,McGovern VL,Le TT,Zaworski PG,Arnold WD,Pellizzoni L,Burghes AHM

Mon Oct 01 00:00:00 UTC 2018

Baseline results of the NeuroNEXT spinal muscular atrophy infant biomarker study.

Annals of clinical and translational neurology

Kolb SJ,Coffey CS,Yankey JW,Krosschell K,Arnold WD,Rutkove SB,Swoboda KJ,Reyna SP,Sakonju A,Darras BT,Shell R,Kuntz N,Castro D,Iannaccone ST,Parsons J,Connolly AM,Chiriboga CA,McDonald C,Burnette WB,Werner K,Thangarajh M,Shieh PB,Finanger E,Cudkowicz ME,McGovern MM,McNeil DE,Finkel R,Kaye E,Kingsley A,Renusch SR,McGovern VL,Wang X,Zaworski PG,Prior TW,Burghes AH,Bartlett A,Kissel JT

Mon Feb 01 00:00:00 UTC 2016

Baseline results of the NeuroNEXT spinal muscular atrophy infant biomarker study.

Annals of clinical and translational neurology

Kolb SJ,Coffey CS,Yankey JW,Krosschell K,Arnold WD,Rutkove SB,Swoboda KJ,Reyna SP,Sakonju A,Darras BT,Shell R,Kuntz N,Castro D,Iannaccone ST,Parsons J,Connolly AM,Chiriboga CA,McDonald C,Burnette WB,Werner K,Thangarajh M,Shieh PB,Finanger E,Cudkowicz ME,McGovern MM,McNeil DE,Finkel R,Kaye E,Kingsley A,Renusch SR,McGovern VL,Wang X,Zaworski PG,Prior TW,Burghes AH,Bartlett A,Kissel JT

Mon Feb 01 00:00:00 UTC 2016

SMN Protein Can Be Reliably Measured in Whole Blood with an Electrochemiluminescence (ECL) Immunoassay: Implications for Clinical Trials.

PloS one

Zaworski P,von Herrmann KM,Taylor S,Sunshine SS,McCarthy K,Risher N,Newcomb T,Weetall M,Prior TW,Swoboda KJ,Chen KS,Paushkin S

Mon Aug 08 00:00:00 UTC 2016

SMN Protein Can Be Reliably Measured in Whole Blood with an Electrochemiluminescence (ECL) Immunoassay: Implications for Clinical Trials.

PloS one

Zaworski P,von Herrmann KM,Taylor S,Sunshine SS,McCarthy K,Risher N,Newcomb T,Weetall M,Prior TW,Swoboda KJ,Chen KS,Paushkin S

Mon Aug 08 00:00:00 UTC 2016

SMN expression is required in motor neurons to rescue electrophysiological deficits in the SMNΔ7 mouse model of SMA.

Human molecular genetics

McGovern VL,Iyer CC,Arnold WD,Gombash SE,Zaworski PG,Blatnik AJ,Foust KD,Burghes AH

Thu Oct 01 00:00:00 UTC 2015

SMN expression is required in motor neurons to rescue electrophysiological deficits in the SMNΔ7 mouse model of SMA.

Human molecular genetics

McGovern VL,Iyer CC,Arnold WD,Gombash SE,Zaworski PG,Blatnik AJ,Foust KD,Burghes AH

Thu Oct 01 00:00:00 UTC 2015

Low levels of Survival Motor Neuron protein are sufficient for normal muscle function in the SMNΔ7 mouse model of SMA.

Human molecular genetics

Iyer CC,McGovern VL,Murray JD,Gombash SE,Zaworski PG,Foust KD,Janssen PM,Burghes AH

Sun Nov 01 00:00:00 UTC 2015

Low levels of Survival Motor Neuron protein are sufficient for normal muscle function in the SMNΔ7 mouse model of SMA.

Human molecular genetics

Iyer CC,McGovern VL,Murray JD,Gombash SE,Zaworski PG,Foust KD,Janssen PM,Burghes AH

Sun Nov 01 00:00:00 UTC 2015

Detection of survival motor neuron protein in buccal cells through electrochemiluminescence-based assay.

Assay and drug development technologies

Steinkellner H,Etzler J,Gmeiner BM,Laccone F

Wed Apr 01 00:00:00 UTC 2015

Detection of survival motor neuron protein in buccal cells through electrochemiluminescence-based assay.

Assay and drug development technologies

Steinkellner H,Etzler J,Gmeiner BM,Laccone F

Wed Apr 01 00:00:00 UTC 2015

Evaluation of SMN protein, transcript, and copy number in the biomarkers for spinal muscular atrophy (BforSMA) clinical study.

PloS one

Crawford TO,Paushkin SV,Kobayashi DT,Forrest SJ,Joyce CL,Finkel RS,Kaufmann P,Swoboda KJ,Tiziano D,Lomastro R,Li RH,Trachtenberg FL,Plasterer T,Chen KS

Mon Sep 10 00:00:00 UTC 2012

Evaluation of SMN protein, transcript, and copy number in the biomarkers for spinal muscular atrophy (BforSMA) clinical study.

PloS one

Crawford TO,Paushkin SV,Kobayashi DT,Forrest SJ,Joyce CL,Finkel RS,Kaufmann P,Swoboda KJ,Tiziano D,Lomastro R,Li RH,Trachtenberg FL,Plasterer T,Chen KS

Mon Sep 10 00:00:00 UTC 2012

Utility of survival motor neuron ELISA for spinal muscular atrophy clinical and preclinical analyses.

PloS one

Kobayashi DT,Olson RJ,Sly L,Swanson CJ,Chung B,Naryshkin N,Narasimhan J,Bhattacharyya A,Mullenix M,Chen KS

Wed Apr 25 00:00:00 UTC 2012

Utility of survival motor neuron ELISA for spinal muscular atrophy clinical and preclinical analyses.

PloS one

Kobayashi DT,Olson RJ,Sly L,Swanson CJ,Chung B,Naryshkin N,Narasimhan J,Bhattacharyya A,Mullenix M,Chen KS

Wed Apr 25 00:00:00 UTC 2012

Utility of survival motor neuron ELISA for spinal muscular atrophy clinical and preclinical analyses.

PloS one

Kobayashi DT,Olson RJ,Sly L,Swanson CJ,Chung B,Naryshkin N,Narasimhan J,Bhattacharyya A,Mullenix M,Chen KS

Wed Apr 25 00:00:00 UTC 2012

Bioinformatics

Protein Aliases: Component of gems 1; Gemin-1; survival motor neuron 1; survival motor neuron 1 protein; Survival motor neuron protein; survival of motor neuron protein; tudor domain containing 16A

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Gene Aliases: AI849087; BCD541; GEMIN1; SMA; SMA1; SMA2; SMA3; SMA4; SMA@; SMN; SMN1; SMN2; SMNC; SMNT; T-BCD541; TDRD16A

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UniProt ID: (Human) Q16637, (Mouse) P97801, (Rat) O35876

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Entrez Gene ID: (Human) 6606, (Mouse) 20595, (Rat) 64301

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RNA binding protein binding identical protein binding fibroblast growth factor binding

spliceosomal complex assembly spliceosomal snRNP assembly DNA-templated transcription, termination nervous system development nuclear import mRNA processing microtubule depolymerization axonogenesis RNA splicing regulation of neuron projection development chemical synaptic transmission

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If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*

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