Proteintech

SMPD1/ASM Polyclonal Antibody

SMPD1/ASM Antibody in Immunohistochemistry (Paraffin) (IHC (P))

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SMPD1/ASM Antibody (14609-1-AP) in IHC (P)

Immunohistochemistry of paraffin-embedded human tonsillitis tissue slide using 14609-1-AP (SMPD1,ASM Antibody) at dilution of 1:200 (under 40x lens). heat mediated antigen retrieved with Tris-EDTA buffer (pH 9).

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Product Details

14609-1-AP

Applications

Tested Dilution

Publications

Western Blot (WB)

1:200-1:1,000

View 9 publications 9 publications

Immunohistochemistry (IHC)

View 3 publications 3 publications

Immunohistochemistry (Paraffin) (IHC (P))

1:100-1:400

Immunocytochemistry (ICC/IF)

View 3 publications 3 publications

Miscellaneous PubMed (MISC)

View 1 publication 1 publication

Not applicable (N/A)

View 1 publication 1 publication

Product Specifications

Species Reactivity

Human, Mouse, Rat

Published species

Human, Mouse

Host/Isotype

Rabbit / IgG

Class

Polyclonal

Type

Antibody

Immunogen

SMPD1,ASM Fusion Protein Ag6180 (47-364 aa encoded by BC041164)

View immunogen

Conjugate

Unconjugated Unconjugated Unconjugated

Form

Liquid

Concentration

0.14 mg/mL

Purification

Antigen affinity chromatography

Storage buffer

PBS, pH 7.3, with 50% glycerol

Contains

0.02% sodium azide

Storage conditions

-20°C

Shipping conditions

Wet ice (domestic); Dry ice (international)

Product Specific Information

Immunogen sequence: LSDSRVLWA PAEAHPLSPQ GHPARLHRIV PRLRDVFGWG NLTCPICKGL FTAINLGLKK EPNVARVGSV AIKLCNLLKI APPAVCQSIV HLFEDDMVEV WRRSVLSPSE ACGLLLGSTC GHWDIFSSWN ISLPTVPKPP PKPPSPPAPG APVSRILFLT DLHWDHDYLE GTDPDCADPL CCRRGSGLPP ASRPGAGYWG EYSKCDLPLR TLESLLSGLG PAGPFDMVYW TGDIPAHDVW HQTRQDQLRA LTTVTALVRK FLGPVPVYPA VGNHESTPVN SFPPPFIEGN HSSRWLYEAM AKAWEPWLPA EALRTLRCI (47-364 aa encoded by BC041164)

Target Information

The protein encoded by this gene is a lysosomal acid sphingomyelinase that converts sphingomyelin to ceramide. The encoded protein also has phospholipase C activity. Defects in this gene are a cause of Niemann-Pick disease type A (NPA) and Niemann-Pick disease type B (NPB). Three transcript variants encoding two different isoforms have been found for this gene.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

Product References

Polyphyllin D punctures hypertrophic lysosomes to reverse drug resistance of hepatocellular carcinoma by targeting acid sphingomyelinase.

Molecular therapy : the journal of the American Society of Gene Therapy

Wang Y,Chen YY,Gao GB,Zheng YH,Yu NN,Ouyang L,Gao X,Li N,Wen SY,Huang S,Zhao Q,Liu L,Cao M,Zhang S,Zhang J,He QY

Wed Jul 05 00:00:00 UTC 2023

Polyphyllin D punctures hypertrophic lysosomes to reverse drug resistance of hepatocellular carcinoma by targeting acid sphingomyelinase.

Molecular therapy : the journal of the American Society of Gene Therapy

Wang Y,Chen YY,Gao GB,Zheng YH,Yu NN,Ouyang L,Gao X,Li N,Wen SY,Huang S,Zhao Q,Liu L,Cao M,Zhang S,Zhang J,He QY

Wed Jul 05 00:00:00 UTC 2023

Proteome-Wide Analysis Reveals TFEB Targets for Establishment of a Prognostic Signature to Predict Clinical Outcomes of Colorectal Cancer.

Cancers

Huang Z,Zhu S,Han Z,Li C,Liang J,Wang Y,Zhang S,Zhang J

Wed Jan 25 00:00:00 UTC 2023

Targeting lysosomal HSP70 induces acid sphingomyelinase-mediated disturbance of lipid metabolism and leads to cell death in T cell malignancies.

Clinical and translational medicine

Qing Y,Guo Y,Zhao Q,Hu P,Li H,Yu X,Zhu M,Wang H,Wang Z,Xu J,Guo Q,Hui H

Wed Mar 01 00:00:00 UTC 2023

Acid sphingomyelinase expression is associated with survival in resectable pancreatic ductal adenocarcinoma.

Journal of molecular medicine (Berlin, Germany)

Wilson GC,Patel SH,Wang J,Xu K,Turner KM,Becker KA,Carpinteiro A,Szabo I,Ahmad SA,Gulbins E

Sat Jul 01 00:00:00 UTC 2023

Ablation of sphingosine kinase 2 suppresses fatty liver-associated hepatocellular carcinoma via downregulation of ceramide transfer protein.

Oncogenesis

Liu XT,Chung LH,Liu D,Chen J,Huang Y,Teo JD,Han XD,Zhao Y,Guan FHX,Tran C,Lee JY,Couttas TA,Liu K,McCaughan GW,Gorrell MD,Don AS,Zhang S,Qi Y

Fri Nov 04 00:00:00 UTC 2022

Involvement of Huanglian Jiedu Decoction on Microglia with Abnormal Sphingolipid Metabolism in Alzheimer's Disease.

Drug design, development and therapy

Qi YY,Heng X,Yao ZY,Qu SY,Ge PY,Zhao X,Ni SJ,Guo R,Yang NY,Zhang QC,Zhu HX

Mon Apr 11 00:00:00 UTC 2022

Lycium barbarum polysaccharides attenuates high glucose-induced diabetic retinal angiogenesis by rescuing the expression of miR-15a-5p in RF/6A cells.

Journal of ethnopharmacology

Zhu Y,Zhao Q,Jiang Y

Sun Jan 30 00:00:00 UTC 2022

Exercise Promotes Pro-Apoptotic Ceramide Signaling in a Mouse Melanoma Model.

Cancers

Lee J,Savage H,Maegawa S,Ballarò R,Pareek S,Guerrouahen BS,Gopalakrishnan V,Schadler K

Fri Sep 02 00:00:00 UTC 2022

Comparative lipidomics of 5-Fluorouracil-sensitive and -resistant colorectal cancer cells reveals altered sphingomyelin and ceramide controlled by acid sphingomyelinase (SMPD1).

Scientific reports

Jung JH,Taniguchi K,Lee HM,Lee MY,Bandu R,Komura K,Lee KY,Akao Y,Kim KP

Thu Apr 09 00:00:00 UTC 2020

Acid sphingomyelinase downregulation alleviates vascular endothelial leptin resistance in rats.

Acta pharmacologica Sinica

Cai BB,Lu YN,Xu M

Fri May 01 00:00:00 UTC 2020

Acid sphingomyelinase downregulation alleviates vascular endothelial leptin resistance in rats.

Acta pharmacologica Sinica

Cai BB,Lu YN,Xu M

Fri May 01 00:00:00 UTC 2020

Acid sphingomyelinase downregulation alleviates vascular endothelial leptin resistance in rats.

Acta pharmacologica Sinica

Cai BB,Lu YN,Xu M

Fri May 01 00:00:00 UTC 2020

Interrelations of Sphingolipid and Lysophosphatidate Signaling with Immune System in Ovarian Cancer.

Computational and structural biotechnology journal

Meshcheryakova A,Svoboda M,Jaritz M,Mungenast F,Salzmann M,Pils D,Cacsire Castillo-Tong D,Hager G,Wolf A,Braicu EI,Sehouli J,Lambrechts S,Vergote I,Mahner S,Birner P,Zimmermann P,Brindley DN,Heinze G,Zeillinger R,Mechtcheriakova D

Wed Feb 14 00:00:00 UTC 2024

The β₂ Integrin Mac-1 Induces Protective LC3-Associated Phagocytosis of Listeria monocytogenes.

Cell host & microbe

Gluschko A,Herb M,Wiegmann K,Krut O,Neiss WF,Utermöhlen O,Krönke M,Schramm M

Wed Mar 14 00:00:00 UTC 2018

The β₂ Integrin Mac-1 Induces Protective LC3-Associated Phagocytosis of Listeria monocytogenes.

Cell host & microbe

Gluschko A,Herb M,Wiegmann K,Krut O,Neiss WF,Utermöhlen O,Krönke M,Schramm M

Wed Mar 14 00:00:00 UTC 2018

Aneuploid Cell Survival Relies upon Sphingolipid Homeostasis.

Cancer research

Tang YC,Yuwen H,Wang K,Bruno PM,Bullock K,Deik A,Santaguida S,Trakala M,Pfau SJ,Zhong N,Huang T,Wang L,Clish CB,Hemann MT,Amon A

Sun Oct 01 00:00:00 UTC 2017

Aneuploid Cell Survival Relies upon Sphingolipid Homeostasis.

Cancer research

Tang YC,Yuwen H,Wang K,Bruno PM,Bullock K,Deik A,Santaguida S,Trakala M,Pfau SJ,Zhong N,Huang T,Wang L,Clish CB,Hemann MT,Amon A

Sun Oct 01 00:00:00 UTC 2017

Aneuploid Cell Survival Relies upon Sphingolipid Homeostasis.

Cancer research

Tang YC,Yuwen H,Wang K,Bruno PM,Bullock K,Deik A,Santaguida S,Trakala M,Pfau SJ,Zhong N,Huang T,Wang L,Clish CB,Hemann MT,Amon A

Sun Oct 01 00:00:00 UTC 2017

Bioinformatics

Protein Aliases: Acid sphingomyelinase; ASMase; Sphingomyelin phosphodiesterase; sphingomyelin phosphodiesterase 1, acid lysosomal

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Gene Aliases: A-SMase; ASM; ASMASE; NPD; SMPD1; Zn-SMase

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UniProt ID: (Human) P17405, (Mouse) Q04519

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Entrez Gene ID: (Human) 6609, (Rat) 308909, (Mouse) 20597

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Function(s)

sphingomyelin phosphodiesterase activity protein binding zinc ion binding hydrolase activity, acting on glycosyl bonds acid sphingomyelin phosphodiesterase activity molecular_function hydrolase activity

Process(es)

sphingomyelin metabolic process sphingomyelin catabolic process glycosphingolipid metabolic process signal transduction nervous system development termination of signal transduction positive regulation of protein dephosphorylation response to cocaine response to drug positive regulation of apoptotic process negative regulation of MAP kinase activity ceramide biosynthetic process metabolic process

Performance Guarantee

If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*

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SMPD1/ASM Polyclonal Antibody

SMPD1/ASM Antibody (14609-1-AP) in IHC (P)

Product Details

14609-1-AP

Western Blot (WB)

Immunohistochemistry (IHC)

Immunohistochemistry (Paraffin) (IHC (P))

Immunocytochemistry (ICC/IF)

Miscellaneous PubMed (MISC)

Not applicable (N/A)

Species Reactivity

Published species

Host/Isotype

Class

Type

Immunogen

Conjugate

Form

Concentration

Purification

Storage buffer

Contains

Storage conditions

Shipping conditions

Product Specific Information

Target Information

Bioinformatics

Performance Guarantee

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