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Invitrogen
SMPD3 Polyclonal Antibody
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SMPD3 Antibody (PA5-143767) in WB
Western blot of SMPD3 in aldehyde-fixed and NP-40-permeabilized differentiated PC12 cells. The cells were labeled with rabbit SMPD3 polyclonal antibody (Product # PA5-143767) in the absence (Left) or presence (Right) of blocking peptide. The antibody was detected using appropriate secondary antibody conjugated to DyLight® 594. The cells were counterstained with Sytox green to label nuclei.
Product Details
PA5-143767
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Human,
Mouse,
Rat
Host/Isotype
Rabbit
/ IgG
Class
Polyclonal
Type
Antibody
Immunogen
nSMase2 synthetic peptide corresponds to amino acids in the C-terminal region of human nSMase2. This sequence is highly conserved in rat and mouse nSMase2, and has low homology to other nSMase family members.
Conjugate
Form
Liquid
Concentration
~0.5 mg/mL
Purification
Antigen affinity chromatography
Storage buffer
PBS with 1mg/mL BSA, 50% glycerol
Contains
0.05% sodium azide
Storage conditions
-20°C, Avoid Freeze/Thaw Cycles
Shipping conditions
Ambient (domestic); Wet ice (international)
RRID
AB_2942995
Product Specific Information
The antibody detects a 78 kDa* band corresponding to nSMase2 on SDS-PAGE immunoblots of adult mouse brain and rat PC12 cells.
Target Information
Catalyzes the hydrolysis of sphingomyelin to form ceramide and phosphocholine. Ceramide mediates numerous cellular functions, such as apoptosis and growth arrest, and is capable of regulating these 2 cellular events independently. Also hydrolyzes sphingosylphosphocholine. Regulates the cell cycle by acting as a growth suppressor in confluent cells. Probably acts as a regulator of postnatal development and participates in bone and dentin mineralization.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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Bioinformatics
Protein Aliases: confluent 3Y1 cell-associated 1; Confluent 3Y1 cell-associated protein 1; neutral sphingomyelin phosphodiesterase 3; Neutral sphingomyelinase 2; Neutral sphingomyelinase II; nSMase-2; nSMase2; Sphingomyelin phosphodiesterase 3; sphingomyelin phosphodiesterase 3, neutral membrane (neutral sphingomyelinase II)
Gene Aliases: 4631433G07Rik; AI427456; AW537966; Cca1; fro; NSMASE2; SMPD3
UniProt ID: (Human) Q9NY59, (Rat) O35049, (Mouse) Q9JJY3
Entrez Gene ID: (Human) 55512, (Rat) 94338, (Mouse) 58994
hematopoietic progenitor cell differentiation
sphingomyelin catabolic process
glycosphingolipid metabolic process
cell cycle
peptide hormone secretion
positive regulation of exosomal secretion
positive regulation of ceramide biosynthetic process
sphingolipid metabolic process
sphingomyelin metabolic process
multicellular organism development
lipid metabolic process
signal transduction
Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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