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Invitrogen
SMURF2 Polyclonal Antibody
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FIGURE: 1 / 4
SMURF2 Antibody (PA5-115937) in ICC/IF
Immunocytochemistry analysis of SMURF2 in HEPG2 cells. Samples were treated with PFA, permeabilized in 0.1% saponin prior to blocking in 10% serum (45 min at 37°C), and incubated with polyclonal antibody (Product # PA5-115937) at a dilution of 1:200 (1 hr at 37°C). Secondary staining was applied with Alexa Fluor 594 conjugated goat anti-rabbit IgG using a dilution of 1:600.
Product Details
PA5-115937
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Human,
Mouse,
Rat
Host/Isotype
Rabbit
/ IgG
Class
Polyclonal
Type
Antibody
Immunogen
A synthesized peptide derived from human SMURF2(Accession Q9HAU4), corresponding to amino acid residues G221-H271.
Conjugate
Form
Liquid
Concentration
1 mg/mL
Purification
Affinity chromatography
Storage buffer
PBS with 50% glycerol
Contains
0.02% sodium azide
Storage conditions
-20°C
Shipping conditions
Wet ice
RRID
AB_2900571
Product Specific Information
Antibody detects endogenous levels of total SMURF2.
Target Information
SMURF2 is an E3 ubiquitin-protein ligase which accepts ubiquitin from an E2 ubiquitin-conjugating enzyme in the form of a thioester and then directly transfers the ubiquitin to targeted substrates. This protein interacts with SMAD1, SMAD2 and SMAD7 in order to trigger their ubiquitination and proteasome-dependent degradation. It enhances the inhibitory activity of SMAD7 and reduces the transcriptional activity of SMAD2. Coexpression of SMURF2 with SMAD1 results in considerable decrease in steady-state level of SMAD1 protein and a smaller decrease of SMAD2 level.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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Bioinformatics
Protein Aliases: E3 ubiquitin ligase SMURF2; E3 ubiquitin-protein ligase SMURF2; HECT-type E3 ubiquitin transferase SMURF2; hSMURF2; SMAD ubiquitination regulatory factor 2; SMAD-specific E3 ubiquitin-protein ligase 2
Gene Aliases: 2810411E22Rik; AI558114; AI649275; RGD1310067; SMURF2
UniProt ID: (Human) Q9HAU4, (Mouse) A2A5Z6
Entrez Gene ID: (Human) 64750, (Rat) 303614, (Mouse) 66313
ubiquitin-protein transferase activity
transforming growth factor beta receptor binding
protein binding
ligase activity
identical protein binding
SMAD binding
ubiquitin protein ligase activity
UDP-N-acetylmuramoylalanyl-D-glutamyl-2,6-diaminopimelate-D-alanyl-D-alanine ligase activity
ribosomal S6-glutamic acid ligase activity
coenzyme F420-0 gamma-glutamyl ligase activity
coenzyme F420-2 alpha-glutamyl ligase activity
protein-glycine ligase activity
protein-glycine ligase activity, initiating
protein-glycine ligase activity, elongating
tubulin-glycine ligase activity
protein-glutamic acid ligase activity
tubulin-glutamic acid ligase activity
negative regulation of transcription from RNA polymerase II promoter
protein polyubiquitination
ubiquitin-dependent protein catabolic process
regulation of transforming growth factor beta receptor signaling pathway
BMP signaling pathway
negative regulation of transforming growth factor beta receptor signaling pathway
ubiquitin-dependent SMAD protein catabolic process
protein ubiquitination involved in ubiquitin-dependent protein catabolic process
negative regulation of transcription, DNA-templated
Wnt signaling pathway, planar cell polarity pathway
positive regulation of canonical Wnt signaling pathway
positive regulation of trophoblast cell migration
transforming growth factor beta receptor signaling pathway
protein ubiquitination
Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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