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Invitrogen
SMYD3 Recombinant Rabbit Monoclonal Antibody (4H2L28)
FIGURE: 1 / 2
SMYD3 Antibody (702519) in WB
Knockdown of SMYD3 was achieved by transfecting HeLa cells with SMYD3 specific siRNA (Silencer® select Product # s34865 and Product # s34863). Western blot analysis (Fig a) was performed using nuclear enriched lysates from the SMYD3 knock down cells (lane 3), non-specific scrambled siRNA transfected cells (lane 2) and untransfected cells (lane 1). The blots were probed with Anti-SMYD3 Recombinant Rabbit Monoclonal Antibody (Product # 702519, 1-2 µg/mL) and Goat anti-Rabbit IgG (Heavy Chain) Superclonal™ Secondary Antibody, HRP conjugate (P... View More
Product Details
702519
Product Specifications
Species Reactivity
Human
Host/Isotype
Rabbit
/ IgG
Expression System
Expi293
Class
Recombinant Monoclonal
Type
Antibody
Clone
4H2L28
Immunogen
Protein corresponding to human SMYD3 (aa1-aa428)
Conjugate
Form
Liquid
Concentration
0.5 mg/mL
Purification
Protein A
Storage buffer
PBS, pH 7.4
Contains
0.09% sodium azide
Storage conditions
Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.
Shipping conditions
Wet ice
RRID
AB_2692254
Product Specific Information
This antibody is predicted to react with Monkey, Rat, Mouse
Recombinant rabbit monoclonal antibodies are produced using in vitro expression systems. The expression systems are developed by cloning in the specific antibody DNA sequences from immunoreactive rabbits. Then, individual clones are screened to select the best candidates for production. The advantages of using recombinant rabbit monoclonal antibodies include: better specificity and sensitivity, lot-to-lot consistency, animal origin-free formulations, and broader immunoreactivity to diverse targets due to larger rabbit immune repertoire.
Target Information
SMYD3 (histone-lysine N-methyltransferase) methylates Lys-4 of histone H3 and Lys-5 of histone H4. It induces di- and tri-methylation. SMYD3 plays an important role in transcriptional activation as a member of an RNA polymerase complex.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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Bioinformatics
Protein Aliases: bA74P14.1 (novel protein); Histone-lysine N-methyltransferase SMYD3; SET and MYND domain-containing protein 3; Zinc finger MYND domain-containing protein 1; zinc finger protein, subfamily 3A (MYND domain containing), 1; zinc finger, MYND domain containing 1
Gene Aliases: bA74P14.1; KMT3E; SMYD3; ZMYND1; ZNFN3A1
UniProt ID: (Human) Q9H7B4
Entrez Gene ID: (Human) 64754
nucleosome assembly
negative regulation of protein kinase activity
myotube cell development
positive regulation of peptidyl-serine phosphorylation
histone lysine methylation
establishment of protein localization
positive regulation of transcription from RNA polymerase II promoter
cellular response to dexamethasone stimulus
Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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