Invitrogen

SNAP25 Polyclonal Antibody

4 Published Figures

7 References

View all (55) SNAP25 antibodies

Datasheet

Protocols

Questions & Answers

Datasheet

Protocols

Questions & Answers

Antibody Testing Data (2)
Published Figures (4)

SNAP25 Antibody in Immunocytochemistry (ICC/IF)

FIGURE: 1 / 6

SNAP25 Antibody (PA1-740) in ICC/IF

Immunlocalization of SNAP-25 in cultured rat hippocampal cells using Product # PA1-740.

SNAP25 Antibody in Immunohistochemistry (IHC)

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Product Details

PA1-740

Applications

Tested Dilution

Publications

Western Blot (WB)

0.5-2 µg/mL

View 1 publication 1 publication

Immunohistochemistry (IHC)

View 3 publications 3 publications

Immunocytochemistry (ICC/IF)

1:100

View 2 publications 2 publications

Neutralization (Neu)

View 1 publication 1 publication

Product Specifications

Species Reactivity

Bovine, Mouse, Rat, Zebrafish

Published species

Chicken, Guinea pig, Mouse, Rat

Host/Isotype

Rabbit / IgG

Class

Polyclonal

Type

Antibody

Immunogen

Synthetic Peptide:(C)- A(195) N Q R A T K M L G S G(206)

View immunogen

Conjugate

Unconjugated Unconjugated Unconjugated

Form

Liquid

Concentration

1 mg/mL

Purification

Antigen affinity chromatography

Storage buffer

PBS with 1mg/mL BSA

Contains

0.05% sodium azide

Storage conditions

-20°C, Avoid Freeze/Thaw Cycles

Shipping conditions

Ambient (domestic); Wet ice (international)

AB_2192212

Product Specific Information

PA1-740 detects synaptosomal associated protein 25 (SNAP-25) from bovine, mouse, rat and zebra fish (Danio rerio) samples.

PA1-740 has been successfully used in Western blot and immunofluorescence procedures. By Western blot, this antibody detects a 25 kDa protein representing SNAP-25 in whole rat brain, mouse brain and zebra fish extracts. Immunofluorescence staining of SNAP-25 in cultured rat hippocampal cells with PA1-740 yields a pattern consistent with cytoplasmic and plasma membrane staining.

PA1-740 immunizing peptide corresponds to the C-terminal amino acid residues 195-206 of mouse SNAP-25. This sequence is completely conserved between mouse, rat, human, chicken, and goldfish. PA1-740 immunizing peptide (Cat. # PEP-032) is available for use in neutralization and control experiments.

Target Information

SNAP25 is a t-SNARE involved in the molecular regulation of neurotransmitter release. It may play a role in the synaptic function of specific neuronal systems. SNAP25 associates with proteins involved in vesicle docking and membrane fusion. It also regulates plasma membrane recycling through its interaction with CENPF. Mutations in the gene can results in myasthenic syndrome.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

Product References

Neuroprotective and Disease-Modifying Effects of the Triazinetrione ACD856, a Positive Allosteric Modulator of Trk-Receptors for the Treatment of Cognitive Dysfunction in Alzheimer's Disease.

International journal of molecular sciences

Parrado Fernandez C,Juric S,Backlund M,Dahlström M,Madjid N,Lidell V,Rasti A,Sandin J,Nordvall G,Forsell P

PA1-740 was used in Immunocytochemistry-immunofluorescence to demonstrate that ACD856 enhanced NGF-induced neurite outgrowth, increased the levels of the pre-synaptic protein SNAP25 in PC12 cells, and increased the degree of phosphorylated TrkB in SH-SY5Y cells.

Thu Jul 06 00:00:00 UTC 2023

Mouse taste cells with G protein-coupled taste receptors lack voltage-gated calcium channels and SNAP-25.

BMC biology

Clapp TR,Medler KF,Damak S,Margolskee RF,Kinnamon SC

PA1-740 was used in blocking/activating experiment to investigate the interaction between synapses with the nervous system and cells sensing various tastes

Thu Mar 30 00:00:00 UTC 2006

Mouse taste cells with G protein-coupled taste receptors lack voltage-gated calcium channels and SNAP-25.

BMC biology

Clapp TR,Medler KF,Damak S,Margolskee RF,Kinnamon SC

PA1-740 was used in blocking/activating experiment to investigate the interaction between synapses with the nervous system and cells sensing various tastes

Thu Mar 30 00:00:00 UTC 2006

Most peptide-containing sensory neurons lack proteins for exocytotic release and vesicular transport of glutamate.

The Journal of comparative neurology

Morris JL,König P,Shimizu T,Jobling P,Gibbins IL

PA1-740 was used in immunohistochemistry to investigate the expression of proteins involved in vesicular uptake and exocytotic release of glutamate in peptide-containing sensory neurons of guinea pigs, mice and toads

Mon Feb 28 00:00:00 UTC 2005

Association of beta-catenin with the alpha-subunit of neuronal large-conductance Ca2+-activated K+ channels.

Proceedings of the National Academy of Sciences of the United States of America

Lesage F,Hibino H,Hudspeth AJ

PA1-740 was used in western blot to study the association of beta-catenin with the alpha-subunit of neuronal large-conductance calcium-activated potassium channels.

Tue Jan 13 00:00:00 UTC 2004

Heterogeneous expression of SNAP-25 and synaptic vesicle proteins by central and peripheral inputs to sympathetic neurons.

The Journal of comparative neurology

Gibbins IL,Jobling P,Teo EH,Matthew SE,Morris JL

Published figure using SNAP25 polyclonal antibody (Product # PA1-740) in Immunofluorescence

Mon Apr 21 00:00:00 UTC 2003

Accumulation of synaptosomal-associated protein of 25 kDa (SNAP-25) and other proteins associated with the secretory pathway in GH4C1 cells upon treatment with estradiol, insulin, and epidermal growth factor.

Endocrinology

Lee MS,Zhu YL,Sun Z,Rhee H,Jeromin A,Roder J,Dannies PS

PA1-740 was used in immunocytochemistry to determine the mechanism for the effects of estradiol, insulin, and epidermal growth factor on SNAP-25 accumulation in GH4C1 cells.

Fri Sep 01 00:00:00 UTC 2000

Bioinformatics

Protein Aliases: ALMS1; blind drunk; CG17676; CG17884; CG40452; Dmel CG40452; Dmel_CG40452; etID17571.20; etID69167.20; FLJ23079; HGNC:11132; SNAP 25; SNAP-25; SNAP-25.1; SNAP-25A; Snap25.1; snp25; SUP; Super protein; Synaptosomal-associated 25 kDa protein; Synaptosomal-associated protein 25; Synaptosomal-associated protein 25-A; synaptosomal-associated protein, 25 kDa; Synaptosome-associated protein 25.1; synaptosome-associated protein 25a

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Gene Aliases: Bdr; BOS_12772; fj42b02; fj45g04; fj48c02; GENA70; si:dkeyp-8f4.6; Snap; SNAP-25; SNAP-25a; SNAP-25B; SNAP25; snap25.1; snap25a; sp; SUP; wu:fj42b02; wu:fj45g04; wu:fj48c02

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UniProt ID: (Bovine) Q17QQ3, (Rat) P60881, (Mouse) P60879, (Zebrafish) Q5TZ66

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Entrez Gene ID: (Bovine) 540853, (Rat) 25012, (Mouse) 20614, (Zebrafish) 30712

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Function(s)

SNARE binding protein binding voltage-gated potassium channel activity SNAP receptor activity myosin binding syntaxin-1 binding protein domain specific binding protein N-terminus binding calcium-dependent protein binding ion channel binding syntaxin binding

Process(es)

exocytosis neurotransmitter secretion axonogenesis long-term memory regulation of neuron projection development synaptic vesicle priming endosomal transport growth hormone secretion sleep synaptic vesicle fusion to presynaptic active zone membrane positive regulation of insulin secretion positive regulation of hormone secretion calcium ion-regulated exocytosis of neurotransmitter regulation of synapse assembly long-term synaptic potentiation regulation of establishment of protein localization potassium ion transmembrane transport locomotory behavior associative learning regulation of ion transmembrane transport mitotic cytokinesis vesicle fusion

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SNAP25 Polyclonal Antibody

SNAP25 Antibody (PA1-740) in ICC/IF

Product Details

PA1-740

Western Blot (WB)

Immunohistochemistry (IHC)

Immunocytochemistry (ICC/IF)

Neutralization (Neu)

Species Reactivity

Published species

Host/Isotype

Class

Type

Immunogen

Conjugate

Form

Concentration

Purification

Storage buffer

Contains

Storage conditions

Shipping conditions

RRID

Product Specific Information

Target Information

Bioinformatics

Performance Guarantee

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