Proteintech

SNAPIN Polyclonal Antibody

SNAPIN Antibody in Immunocytochemistry (ICC/IF)

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SNAPIN Antibody (10055-1-AP) in ICC/IF

Immunofluorescent analysis of HepG2 cells using 10055-1-AP (SNAPIN antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG (H+L).

SNAPIN Antibody in Immunohistochemistry (Paraffin) (IHC (P))

SNAPIN Antibody in Flow Cytometry (Flow)

SNAPIN Antibody in Immunoprecipitation (IP)

Product Image Gallery

Product Details

10055-1-AP

Applications

Tested Dilution

Publications

Western Blot (WB)

1:200-1:1,000

View 11 publications 11 publications

Immunohistochemistry (IHC)

View 2 publications 2 publications

Immunohistochemistry (Paraffin) (IHC (P))

1:50-1:500

Immunocytochemistry (ICC/IF)

1:20-1:200

View 2 publications 2 publications

Flow Cytometry (Flow)

0.2 µg/1x10^6 cells

Immunoprecipitation (IP)

0.5-4.0 µg

Not applicable (N/A)

View 1 publication 1 publication

Product Specifications

Species Reactivity

Human, Mouse, Rat

Published species

Human, Mouse

Host/Isotype

Rabbit / IgG

Class

Polyclonal

Type

Antibody

Immunogen

SNAPIN Fusion Protein Ag0101 (1-136 aa encoded by BC000761)

View immunogen

Conjugate

Unconjugated Unconjugated Unconjugated

Form

Liquid

Concentration

0.35 mg/mL

Purification

Antigen affinity chromatography

Storage buffer

PBS, pH 7.3, with 50% glycerol

Contains

0.02% sodium azide

Storage conditions

-20°C

Shipping conditions

Wet ice (domestic); Dry ice (international)

Product Specific Information

This antibody can recognize the 15-18 kDa (Monomer) and 30-36 kDa (Dimer) forms of SNAPIN.

Immunogen sequence: MAGAGSAAV SGAGTPVAGP TGRDLFAEGL LEFLRPAVQQ LDSHVHAVRE SQVELREQID NLATELCRIN EDQKVALDLD PYVKKLLNAR RRVVLVNNIL QNAQERLRRL NHSVAKETAR RRAMLDSGIY PPGSPGK (1-136 aa encoded by BC000761)

Target Information

The protein encoded by this gene is a coiled-coil-forming protein that associates with the SNARE (soluble N-ethylmaleimide-sensitive fusion protein attachment protein receptor) complex of proteins and the BLOC-1 (biogenesis of lysosome-related organelles) complex. Biochemical studies have identified additional binding partners. As part of the SNARE complex, it is required for vesicle docking and fusion and regulates neurotransmitter release. The BLOC-1 complex is required for the biogenesis of specialized organelles such as melanosomes and platelet dense granules. Mutations in gene products that form the BLOC-1 complex have been identified in mouse strains that are models of Hermansky-Pudlak syndrome. Alternative splicing results in multiple transcript variants.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

Product References

Brucella activates the host RIDD pathway to subvert BLOS1-directed immune defense.

eLife

Wells KM,He K,Pandey A,Cabello A,Zhang D,Yang J,Gomez G,Liu Y,Chang H,Li X,Zhang H,Feng X,da Costa LF,Metz R,Johnson CD,Martin CL,Skrobarczyk J,Berghman LR,Patrick KL,Leibowitz J,Ficht A,Sze SH,Song J,Qian X,Qin QM,Ficht TA,de Figueiredo P

Thu May 19 00:00:00 UTC 2022

HIV-1 Vpr protein impairs lysosome clearance causing SNCA/alpha-synuclein accumulation in neurons.

Autophagy

Santerre M,Arjona SP,Allen CN,Callen S,Buch S,Sawaya BE

Thu Jul 01 00:00:00 UTC 2021

SNAPIN Regulates Cell Cycle Progression to Promote Pancreatic β Cell Growth.

Frontiers in endocrinology

Jiang M,Kuang Z,He Y,Cao Y,Yu T,Cheng J,Liu W,Wang W

Thu Dec 16 00:00:00 UTC 2021

SNAPIN Regulates Cell Cycle Progression to Promote Pancreatic β Cell Growth.

Frontiers in endocrinology

Jiang M,Kuang Z,He Y,Cao Y,Yu T,Cheng J,Liu W,Wang W

Thu Dec 16 00:00:00 UTC 2021

SNAPIN Regulates Cell Cycle Progression to Promote Pancreatic β Cell Growth.

Frontiers in endocrinology

Jiang M,Kuang Z,He Y,Cao Y,Yu T,Cheng J,Liu W,Wang W

Thu Dec 16 00:00:00 UTC 2021

The sperm-associated antigen 6 interactome and its role in spermatogenesis.

Reproduction (Cambridge, England)

Liu Y,Zhang L,Li W,Huang Q,Yuan S,Li Y,Liu J,Zhang S,Pin G,Song S,Ray PF,Arnoult C,Cho C,Garcia-Reyes B,Knippschild U,Strauss JF,Zhang Z

Thu Aug 01 00:00:00 UTC 2019

Biogenesis of lysosome-related organelles complex-1 (BORC) regulates late endosomal/lysosomal size through PIKfyve-dependent phosphatidylinositol-3,5-bisphosphate.

Traffic (Copenhagen, Denmark)

Yordanov TE,Hipolito VEB,Liebscher G,Vogel GF,Stasyk T,Herrmann C,Geley S,Teis D,Botelho RJ,Hess MW,Huber LA

Sun Sep 01 00:00:00 UTC 2019

Carbon nanotubes, but not spherical nanoparticles, block autophagy by a shape-related targeting of lysosomes in murine macrophages.

Autophagy

Cohignac V,Landry MJ,Ridoux A,Pinault M,Annangi B,Gerdil A,Herlin-Boime N,Mayne M,Haruta M,Codogno P,Boczkowski J,Pairon JC,Lanone S

Fri Oct 11 00:00:00 UTC 2019

SNAPIN is critical for lysosomal acidification and autophagosome maturation in macrophages.

Autophagy

Shi B,Huang QQ,Birkett R,Doyle R,Dorfleutner A,Stehlik C,He C,Pope RM

Wed Feb 01 00:00:00 UTC 2017

SNAPIN is critical for lysosomal acidification and autophagosome maturation in macrophages.

Autophagy

Shi B,Huang QQ,Birkett R,Doyle R,Dorfleutner A,Stehlik C,He C,Pope RM

Wed Feb 01 00:00:00 UTC 2017

SNAPIN is critical for lysosomal acidification and autophagosome maturation in macrophages.

Autophagy

Shi B,Huang QQ,Birkett R,Doyle R,Dorfleutner A,Stehlik C,He C,Pope RM

Wed Feb 01 00:00:00 UTC 2017

SNAPIN is critical for lysosomal acidification and autophagosome maturation in macrophages.

Autophagy

Shi B,Huang QQ,Birkett R,Doyle R,Dorfleutner A,Stehlik C,He C,Pope RM

Wed Feb 01 00:00:00 UTC 2017

LAMTOR/Ragulator is a negative regulator of Arl8b- and BORC-dependent late endosomal positioning.

The Journal of cell biology

Filipek PA,de Araujo MEG,Vogel GF,De Smet CH,Eberharter D,Rebsamen M,Rudashevskaya EL,Kremser L,Yordanov T,Tschaikner P,Fürnrohr BG,Lechner S,Dunzendorfer-Matt T,Scheffzek K,Bennett KL,Superti-Furga G,Lindner HH,Stasyk T,Huber LA

Mon Dec 04 00:00:00 UTC 2017

LAMTOR/Ragulator is a negative regulator of Arl8b- and BORC-dependent late endosomal positioning.

The Journal of cell biology

Mon Dec 04 00:00:00 UTC 2017

Dysregulation of PLDN (pallidin) is a mechanism for platelet dense granule deficiency in RUNX1 haplodeficiency.

Journal of thrombosis and haemostasis : JTH

Mao GF,Goldfinger LE,Fan DC,Lambert MP,Jalagadugula G,Freishtat R,Rao AK

Sat Apr 01 00:00:00 UTC 2017

A human skeletal muscle interactome centered on proteins involved in muscular dystrophies: LGMD interactome.

Skeletal muscle

Blandin G,Marchand S,Charton K,Danièle N,Gicquel E,Boucheteil JB,Bentaib A,Barrault L,Stockholm D,Bartoli M,Richard I

Fri Feb 15 00:00:00 UTC 2013

A human skeletal muscle interactome centered on proteins involved in muscular dystrophies: LGMD interactome.

Skeletal muscle

Blandin G,Marchand S,Charton K,Danièle N,Gicquel E,Boucheteil JB,Bentaib A,Barrault L,Stockholm D,Bartoli M,Richard I

Fri Feb 15 00:00:00 UTC 2013

Annexin A1 complex mediates oxytocin vesicle transport.

Journal of neuroendocrinology

Makani V,Sultana R,Sie KS,Orjiako D,Tatangelo M,Dowling A,Cai J,Pierce W,Butterfield DA,Hill J,Park J

Sun Dec 01 00:00:00 UTC 2013

Annexin A1 complex mediates oxytocin vesicle transport.

Journal of neuroendocrinology

Makani V,Sultana R,Sie KS,Orjiako D,Tatangelo M,Dowling A,Cai J,Pierce W,Butterfield DA,Hill J,Park J

Sun Dec 01 00:00:00 UTC 2013

A BLOC-1 mutation screen reveals a novel BLOC1S3 mutation in Hermansky-Pudlak Syndrome type 8.

Pigment cell & melanoma research

Cullinane AR,Curry JA,Golas G,Pan J,Carmona-Rivera C,Hess RA,White JG,Huizing M,Gahl WA

Sat Sep 01 00:00:00 UTC 2012

A BLOC-1 mutation screen reveals a novel BLOC1S3 mutation in Hermansky-Pudlak Syndrome type 8.

Pigment cell & melanoma research

Cullinane AR,Curry JA,Golas G,Pan J,Carmona-Rivera C,Hess RA,White JG,Huizing M,Gahl WA

Sat Sep 01 00:00:00 UTC 2012

A BLOC-1 mutation screen reveals that PLDN is mutated in Hermansky-Pudlak Syndrome type 9.

American journal of human genetics

Cullinane AR,Curry JA,Carmona-Rivera C,Summers CG,Ciccone C,Cardillo ND,Dorward H,Hess RA,White JG,Adams D,Huizing M,Gahl WA

Fri Jun 10 00:00:00 UTC 2011

A BLOC-1 mutation screen reveals that PLDN is mutated in Hermansky-Pudlak Syndrome type 9.

American journal of human genetics

Cullinane AR,Curry JA,Carmona-Rivera C,Summers CG,Ciccone C,Cardillo ND,Dorward H,Hess RA,White JG,Adams D,Huizing M,Gahl WA

Fri Jun 10 00:00:00 UTC 2011

Bioinformatics

Protein Aliases: biogenesis of lysosomal organelles complex-1, subunit 7; Biogenesis of lysosome-related organelles complex 1 subunit 7; BLOC-1 subunit 7; SNAP-25-binding protein; SNAP-associated protein; SNARE associated protein snapin; SNARE-associated protein Snapin; synaptosomal-associated protein 25 binding protein; Synaptosomal-associated protein 25-binding protein; synaptosomal-associated protein, 25 kDa, binding protein

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Gene Aliases: 25kDa; AA407989; AV026596; BLOC1S7; BLOS7; BORCS3; SNAP25BP; SNAPAP; SNAPIN

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UniProt ID: (Human) O95295, (Mouse) Q9Z266, (Rat) P60192

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Entrez Gene ID: (Human) 23557, (Mouse) 20615, (Rat) 295217

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Function(s)

SNARE binding protein binding

Process(es)

intracellular protein transport neurotransmitter secretion anterograde axonal transport endosome to lysosome transport negative regulation of neuron projection development viral process synaptic vesicle exocytosis synaptic vesicle maturation neuron projection development synaptic vesicle fusion to presynaptic active zone membrane lysosome localization melanosome organization regulation of protein binding synaptic vesicle transport anterograde synaptic vesicle transport terminal button organization autophagosome maturation positive regulation of late endosome to lysosome transport exocytosis chemical synaptic transmission calcium ion regulated exocytosis

Performance Guarantee

If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*

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SNAPIN Polyclonal Antibody

SNAPIN Antibody (10055-1-AP) in ICC/IF

Product Details

10055-1-AP

Western Blot (WB)

Immunohistochemistry (IHC)

Immunohistochemistry (Paraffin) (IHC (P))

Immunocytochemistry (ICC/IF)

Flow Cytometry (Flow)

Immunoprecipitation (IP)

Not applicable (N/A)

Species Reactivity

Published species

Host/Isotype

Class

Type

Immunogen

Conjugate

Form

Concentration

Purification

Storage buffer

Contains

Storage conditions

Shipping conditions

Product Specific Information

Target Information

Bioinformatics

Performance Guarantee

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