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Invitrogen
SNRPA Polyclonal Antibody
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SNRPA Antibody (PA5-27474) in ICC/IF
Immunofluorescence analysis of SNRPA was performed using 70% confluent log phase HeLa cells. The cells were fixed with 4% paraformaldehyde for 10 minutes, permeabilized with 0.1% Triton™ X-100 for 15 minutes, and blocked with 1% BSA for 1 hour at room temperature. The cells were labeled with SNRPA Polyclonal Antibody (Product # PA5-27474) at 5 µg/mL concentration in 0.1% BSA, incubated at 4 degree Celsius overnight and then labeled with Goat anti-Rabbit IgG (Heavy Chain) Superclonal™ Secondary Antibody, Alexa Fluor® 488 conjugate (Product ... View More
Product Details
PA5-27474
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Human,
Mouse,
Rat
Published species
Human
Host/Isotype
Rabbit
/ IgG
Class
Polyclonal
Type
Antibody
Immunogen
Recombinant protein encompassing a sequence within the C-terminus region of human SNRPA. The exact sequence is proprietary.
Conjugate
Form
Liquid
Concentration
1 mg/mL
Purification
Antigen affinity chromatography
Storage buffer
PBS, pH 7, with 1% BSA, 20% glycerol
Contains
0.01% thimerosal
Storage conditions
Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.
Shipping conditions
Ambient (domestic); Wet ice (international)
RRID
AB_2544950
Product Specific Information
Recommended positive controls: A549, HeLa, HepG2.
Predicted reactivity: Mouse (100%), Rat (100%), Zebrafish (89%), Xenopus laevis (91%), Pig (100%), Rabbit (98%), Sheep (100%), Rhesus Monkey (100%), Bovine (100%).
Store product as a concentrated solution. Centrifuge briefly prior to opening the vial.
Target Information
The protein encoded by this gene associates with stem loop II of the U1 small nuclear ribonucleoprotein, which binds the 5' splice site of precursor mRNAs and is required for splicing. The encoded protein autoregulates itself by polyadenylation inhibition of its own pre-mRNA via dimerization and has been implicated in the coupling of splicing and polyadenylation.
⚠WARNING: This product can expose you to chemicals including mercury, which is known to the State of California to cause birth defects or other reproductive harm. For more information go to www.P65Warnings.ca.gov.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
Bioinformatics
Protein Aliases: protein A of the U1 small nuclear RNA; U1 small nuclear ribonucleoprotein A; U1 small nuclear RNP-specific A; U1 snRNP A; U1 snRNP-specific protein A
Gene Aliases: C430021M15Rik; Mud1; Rnu1a-1; Rnu1a1; SNRPA; U1-A; U1A
UniProt ID: (Human) P09012, (Mouse) Q62189
Entrez Gene ID: (Human) 6626, (Mouse) 53607, (Rat) 292729
Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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